Catalog No.
YHE31101
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Lys429-Val488
Predicted molecular weight
8.84 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P41226
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
D8, Ubiquitin-activating enzyme 7, UBE1L, UBA7, Ubiquitin-like modifier-activating enzyme 7, UBE2, Ubiquitin-activating enzyme E1 homolog
HERC5-mediated ISGylation of SARS-CoV-2 nsp8 facilitates its degradation and inhibits viral replication., PMID:40409630
Ubiquitin-proteasome Pathway-linked Gene Signatures as Prognostic Indicators in Prostate Cancer., PMID:40295064
Identification of UBA7 expression downregulation in myelodysplastic neoplasm with SF3B1 mutations., PMID:40158006
ISG15 Drives Immune Pathology and Respiratory Failure during Systemic Lymphocytic Choriomeningitis Virus Infection., PMID:39495004
CREBRF regulates apoptosis and estradiol via ISG15/ISGylation in pig granulosa cells., PMID:39419455
Characterizing the Monomer-Dimer Equilibrium of UbcH8/Ube2L6: A Combined SAXS and NMR Study., PMID:39346869
ISGylation enhances dsRNA-induced interferon response and NFκB signaling in fallopian tube epithelial cells., PMID:39159817
Insights into the ISG15 transfer cascade by the UBE1L activating enzyme., PMID:38042859
ISGylation-independent protection of cell growth by USP18 following interferon stimulation., PMID:37756534
Cryo-EM structures of Uba7 reveal the molecular basis for ISG15 activation and E1-E2 thioester transfer., PMID:37553340
A plasma proteomic approach in patients with heart failure after acute myocardial infarction: insights into the pathogenesis and progression of the disease., PMID:37265567
ISG15 suppresses ovulation and female fertility by ISGylating ADAMTS1., PMID:37170317
Characterizing the monomer-dimer equilibrium of UbcH8/Ube2L6: A combined SAXS and NMR study., PMID:37090523
Simultaneous capture of ISG15 conjugating and deconjugating enzymes using a semi-synthetic ISG15-Dha probe., PMID:36684644
Downregulation of SOCS1 increases interferon-induced ISGylation during differentiation of induced-pluripotent stem cells to hepatocytes., PMID:36439639
The comprehensive detection of miRNA and circRNA in the regulation of intramuscular and subcutaneous adipose tissue of Laiwu pig., PMID:36192451
Integrated Multi-Omics Signature Predicts Survival in Head and Neck Cancer., PMID:36010616
Insights into the evolution of the ISG15 and UBA7 system., PMID:35134494
Identification of Hub Genes Associated With Tuberculous Pleurisy by Integrated Bioinformatics Analysis., PMID:34925441
Pet ownership in pregnancy and methylation pattern in cord blood., PMID:34642452
More than Meets the ISG15: Emerging Roles in the DNA Damage Response and Beyond., PMID:33203188
E1 Enzymes as Therapeutic Targets in Cancer., PMID:33177128
Targeting a helix-in-groove interaction between E1 and E2 blocks ubiquitin transfer., PMID:32807965
Unstructured Biology of Proteins from Ubiquitin-Proteasome System: Roles in Cancer and Neurodegenerative Diseases., PMID:32455657
Direct Antiviral Activity of IFN-Stimulated Genes Is Responsible for Resistance to Paramyxoviruses in ISG15-Deficient Cells., PMID:32423918
Type I Interferon Regulates a Coordinated Gene Network to Enhance Cytotoxic T Cell-Mediated Tumor Killing., PMID:31974171
Up-regulation of interferon-stimulated gene 15 and its conjugation machinery, UbE1L and UbcH8 expression by tumor necrosis factor-α through p38 MAPK and JNK signaling pathways in human lung carcinoma., PMID:31428903
A Comprehensive Bioinformatics Analysis of UBE2C in Cancers., PMID:31067633
Functional characterization of 3D protein structures informed by human genetic diversity., PMID:30988206
Proteomic analysis of RAW macrophages treated with cGAMP or c-di-GMP reveals differentially activated cellular pathways., PMID:35558957
Furosine Induced Apoptosis by the Regulation of STAT1/STAT2 and UBA7/UBE2L6 Genes in HepG2 Cells., PMID:29857509
Transmembrane Protein pUL50 of Human Cytomegalovirus Inhibits ISGylation by Downregulating UBE1L., PMID:29743376
Inhibition of rabies virus replication by interferon-stimulated gene 15 and its activating enzyme UBA7., PMID:29056542
Interferon-Alpha Reduces Human Hippocampal Neurogenesis and Increases Apoptosis via Activation of Distinct STAT1-Dependent Mechanisms., PMID:29040650
ISG'ylation increases stability of numerous proteins including Stat1, which prevents premature termination of immune response in LPS-stimulated microglia., PMID:28774718
ISG15 inhibits cancer cell growth and promotes apoptosis., PMID:28035359
Interferon-Stimulated Gene 15 Conjugation Stimulates Hepatitis B Virus Production Independent of Type I Interferon Signaling Pathway In Vitro., PMID:27867263
Positive feedback regulation of p53 transactivity by DNA damage-induced ISG15 modification., PMID:27545325
AG490 and PF431396 Sensitive Tyrosine Kinase Control the Population Heterogeneity of Basal STAT1 Activity in Ube1l Deficient Cells., PMID:27427993
Gene expression profiling reveals key genes and pathways related to the development of non-alcoholic fatty liver disease., PMID:26845596
ISG15 Is Upregulated in Respiratory Syncytial Virus Infection and Reduces Virus Growth through Protein ISGylation., PMID:26763998
Modification of BECN1 by ISG15 plays a crucial role in autophagy regulation by type I IFN/interferon., PMID:25906440
Host genetic variation influences gene expression response to rhinovirus infection., PMID:25874939
Mechanism of Dose-Dependent Regulation of UBE1L by Polyphenols in Human Bronchial Epithelial Cells., PMID:25649257
The signal transducers Stat1 and Stat3 and their novel target Jmjd3 drive the expression of inflammatory genes in microglia., PMID:24097101
Repression of exogenous gene expression by the retinoic acid target gene G0S2., PMID:23546556
[Study on gene differential expressions of substance and energy metabolism in chronic superficial gastritis patients of Pi deficiency syndrome and of pi-wei hygropyrexia syndrome]., PMID:23185754
The ISG15 conjugation system., PMID:22350882
ISG15 is critical in the control of Chikungunya virus infection independent of UbE1L mediated conjugation., PMID:22028657
Increased cerebral protein ISGylation after focal ischemia is neuroprotective., PMID:21847135
For research use only. Not for human or drug use.
