A single-chain antibody-based AID2 system for conditional degradation of GFP-tagged and untagged proteins., PMID:40485297
Development and characterization of a novel NEIL1 nanobody., PMID:40440929
Growth inhibition of Saccharomyces cerevisiae by SUMO-specific nanobodies., PMID:40419540
Red Light-Activated Reversible Inhibition of Protein Functions by Assembled Trap., PMID:40304578
High-throughput optimization of peptide-linker for fusing function protein with GFP., PMID:40239936
PHluorin-conjugated secondary nanobodies as a tool for measuring synaptic vesicle exocytosis and endocytosis., PMID:40128278
Regulating ferredoxin electron transfer using nanobody and antigen interactions., PMID:40059882
Direct observation of fluorescent proteins in gels: A rapid, cost-efficient, and quantitative alternative to immunoblotting., PMID:39924827
Nanobody-thioesterase chimeras to specifically target protein palmitoylation., PMID:39920166
TAZ interactome analysis using nanotrap-based affinity purification-mass spectrometry., PMID:39898439
Peptidisc-Assisted Hydrophobic Clustering Toward the Production of Multimeric and Multispecific Nanobody Proteins., PMID:39810388
Structure-based design of covalent nanobody binders for a thermostable green fluorescence protein., PMID:39719878
Development of Etf-3-specific nanobodies to prevent Ehrlichia infection and LNP-mRNA delivery in cellular and murine models., PMID:39705831
Delivery of Peptide Coacervates to Form Stable Interaction Hubs in Cells., PMID:39651133
Uncovering the electrical synapse proteome in retinal neurons via in vivo proximity labeling., PMID:39651118
A nanobody-based microfluidic chip for fast and automated purification of protein complexes., PMID:39588652
Decoding polyubiquitin regulation of KV7. 1 functional expression with engineered linkage-selective deubiquitinases., PMID:39345403
Destabilized near-infrared fluorescent nanobodies enable background-free targeting of GFP-based biosensors for imaging and manipulation., PMID:39242569
A phosphorylation-controlled switch confers cell cycle-dependent protein relocalization., PMID:39209962
Aggregate-selective removal of pathological tau by clustering-activated degraders., PMID:39208111
Protein Carrier AAV., PMID:39185209
Fast In Vitro Synthesis and Direct Labeling of Nanobodies for Prototyping in Microscopy Applications., PMID:39184460
Artificial targeting of autophagy components to mitochondria reveals both conventional and unconventional mitophagy pathways., PMID:39177530
A new generation of nanobody research tools using improved mass spectrometry-based discovery methods., PMID:39098531
A Single-step Generation of AlissAID-based Conditional Knockdown Strains Using Nanobody that Targets GFP or mCherry in Budding Yeast., PMID:38948262
Functional analysis of protein interactions using coupled bi-fluorescence complementation/GFP nanobody techniques., PMID:38932691
A phosphorylation-controlled switch confers cell cycle-dependent protein relocalization., PMID:38895347
Development of a split-luciferase assay to establish optimal protein secretion conditions for protein production by Bacillus subtilis., PMID:38847798
Milk-derived extracellular vesicles functionalized with anti-tumour necrosis factor-α nanobody and anti-microbial peptide alleviate ulcerative colitis in mice., PMID:38840457
Engineering and characterization of GFP-targeting nanobody: Expression, purification, and post-translational modification analysis., PMID:38782081
Adhesion energy controls lipid binding-mediated endocytosis., PMID:38553473
FoxP1 Represses MEF2A in Striated Muscle., PMID:38483114
PBRM-1/PBAF-regulated genes in a multipotent progenitor in Caenorhabditis elegans., PMID:38150396
A nanobody-based strategy for rapid and scalable purification of human protein complexes., PMID:37974029
Mirror-Image Single-Domain Antibody for a Novel Nonimmunogenic Drug Scaffold., PMID:37883660
Cellular Assays for Dynamic Quantification of Deubiquitinase Activity and Inhibition., PMID:37858708
Photoswitchable binders enable temporal dissection of endogenous protein function., PMID:37745504
Chemical Acetylation of Ligands and Two-Step Digestion Protocol for Reducing Codigestion in Affinity Purification-Mass Spectrometry., PMID:37712406
Divergent regulation of KCNQ1/E1 by targeted recruitment of protein kinase A to distinct sites on the channel complex., PMID:37650513
Visualizing the subcellular localization of RHOB-GTP and GTPase-Effector complexes using a split-GFP/nanobody labelling assay., PMID:37639782
Identification of KLHDC2 as an efficient proximity-induced degrader of K-RAS, STK33, β-catenin, and FoxP3., PMID:37591251
Thermostability enhancement of polyethylene terephthalate degrading PETase using self- and nonself-ligating protein scaffolding approaches., PMID:37555384
Adhesion energy controls lipid binding-mediated endocytosis., PMID:37503169
Super-resolution proximity labeling reveals anti-viral protein network and its structural changes against SARS-CoV-2 viral proteins., PMID:37478010
Selection, identification and crystal structure of shark-derived single-domain antibodies against a green fluorescent protein., PMID:37460076
The transcriptional regulator Ume6 is a major driver of early gene expression during gametogenesis., PMID:37431893
Extreme thermal stability of the antiGFP nanobody - GFP complex., PMID:37340471
Development of AlissAID system targeting GFP or mCherry fusion protein., PMID:37315088
Subunit-Dependent Surface Mobility and Localization of NMDA Receptors in Hippocampal Neurons Measured Using Nanobody Probes., PMID:37286354
Development and characterization of nanobodies that specifically target the oncogenic Phosphatase of Regenerating Liver-3 (PRL-3) and impact its interaction with a known binding partner, CNNM3., PMID:37220097