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Anti-GFP Nanobody (SAA0976)

Catalog #:   RWK25805 Specific References (50) DATASHEET
Host species: Alpaca
Isotype: VHH-8His-Cys-tag
Applications: ELISA
Expression system: Mammalian Cells
Overview

Catalog No.

RWK25805

Expression system

Mammalian Cells

Species reactivity

Aequorea victoria (Water jellyfish) (Mesonema victoria)

Host species

Alpaca

Isotype

VHH-8His-Cys-tag

Clonality

Monoclonal

Target

Green fluorescent protein, GFP

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P42212

Applications

ELISA

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

SAA0976

Data Image
  • SDS-PAGE
    SDS PAGE for GFP Nanobody (SAA0976)
References

A single-chain antibody-based AID2 system for conditional degradation of GFP-tagged and untagged proteins., PMID:40485297

Development and characterization of a novel NEIL1 nanobody., PMID:40440929

Growth inhibition of Saccharomyces cerevisiae by SUMO-specific nanobodies., PMID:40419540

Red Light-Activated Reversible Inhibition of Protein Functions by Assembled Trap., PMID:40304578

High-throughput optimization of peptide-linker for fusing function protein with GFP., PMID:40239936

PHluorin-conjugated secondary nanobodies as a tool for measuring synaptic vesicle exocytosis and endocytosis., PMID:40128278

Regulating ferredoxin electron transfer using nanobody and antigen interactions., PMID:40059882

Direct observation of fluorescent proteins in gels: A rapid, cost-efficient, and quantitative alternative to immunoblotting., PMID:39924827

Nanobody-thioesterase chimeras to specifically target protein palmitoylation., PMID:39920166

TAZ interactome analysis using nanotrap-based affinity purification-mass spectrometry., PMID:39898439

Peptidisc-Assisted Hydrophobic Clustering Toward the Production of Multimeric and Multispecific Nanobody Proteins., PMID:39810388

Structure-based design of covalent nanobody binders for a thermostable green fluorescence protein., PMID:39719878

Development of Etf-3-specific nanobodies to prevent Ehrlichia infection and LNP-mRNA delivery in cellular and murine models., PMID:39705831

Delivery of Peptide Coacervates to Form Stable Interaction Hubs in Cells., PMID:39651133

Uncovering the electrical synapse proteome in retinal neurons via in vivo proximity labeling., PMID:39651118

A nanobody-based microfluidic chip for fast and automated purification of protein complexes., PMID:39588652

Decoding polyubiquitin regulation of KV7. 1 functional expression with engineered linkage-selective deubiquitinases., PMID:39345403

Destabilized near-infrared fluorescent nanobodies enable background-free targeting of GFP-based biosensors for imaging and manipulation., PMID:39242569

A phosphorylation-controlled switch confers cell cycle-dependent protein relocalization., PMID:39209962

Aggregate-selective removal of pathological tau by clustering-activated degraders., PMID:39208111

Protein Carrier AAV., PMID:39185209

Fast In Vitro Synthesis and Direct Labeling of Nanobodies for Prototyping in Microscopy Applications., PMID:39184460

Artificial targeting of autophagy components to mitochondria reveals both conventional and unconventional mitophagy pathways., PMID:39177530

A new generation of nanobody research tools using improved mass spectrometry-based discovery methods., PMID:39098531

A Single-step Generation of AlissAID-based Conditional Knockdown Strains Using Nanobody that Targets GFP or mCherry in Budding Yeast., PMID:38948262

Functional analysis of protein interactions using coupled bi-fluorescence complementation/GFP nanobody techniques., PMID:38932691

A phosphorylation-controlled switch confers cell cycle-dependent protein relocalization., PMID:38895347

Development of a split-luciferase assay to establish optimal protein secretion conditions for protein production by Bacillus subtilis., PMID:38847798

Milk-derived extracellular vesicles functionalized with anti-tumour necrosis factor-α nanobody and anti-microbial peptide alleviate ulcerative colitis in mice., PMID:38840457

Engineering and characterization of GFP-targeting nanobody: Expression, purification, and post-translational modification analysis., PMID:38782081

Adhesion energy controls lipid binding-mediated endocytosis., PMID:38553473

FoxP1 Represses MEF2A in Striated Muscle., PMID:38483114

PBRM-1/PBAF-regulated genes in a multipotent progenitor in Caenorhabditis elegans., PMID:38150396

A nanobody-based strategy for rapid and scalable purification of human protein complexes., PMID:37974029

Mirror-Image Single-Domain Antibody for a Novel Nonimmunogenic Drug Scaffold., PMID:37883660

Cellular Assays for Dynamic Quantification of Deubiquitinase Activity and Inhibition., PMID:37858708

Photoswitchable binders enable temporal dissection of endogenous protein function., PMID:37745504

Chemical Acetylation of Ligands and Two-Step Digestion Protocol for Reducing Codigestion in Affinity Purification-Mass Spectrometry., PMID:37712406

Divergent regulation of KCNQ1/E1 by targeted recruitment of protein kinase A to distinct sites on the channel complex., PMID:37650513

Visualizing the subcellular localization of RHOB-GTP and GTPase-Effector complexes using a split-GFP/nanobody labelling assay., PMID:37639782

Identification of KLHDC2 as an efficient proximity-induced degrader of K-RAS, STK33, β-catenin, and FoxP3., PMID:37591251

Thermostability enhancement of polyethylene terephthalate degrading PETase using self- and nonself-ligating protein scaffolding approaches., PMID:37555384

Adhesion energy controls lipid binding-mediated endocytosis., PMID:37503169

Super-resolution proximity labeling reveals anti-viral protein network and its structural changes against SARS-CoV-2 viral proteins., PMID:37478010

Selection, identification and crystal structure of shark-derived single-domain antibodies against a green fluorescent protein., PMID:37460076

The transcriptional regulator Ume6 is a major driver of early gene expression during gametogenesis., PMID:37431893

Extreme thermal stability of the antiGFP nanobody - GFP complex., PMID:37340471

Development of AlissAID system targeting GFP or mCherry fusion protein., PMID:37315088

Subunit-Dependent Surface Mobility and Localization of NMDA Receptors in Hippocampal Neurons Measured Using Nanobody Probes., PMID:37286354

Development and characterization of nanobodies that specifically target the oncogenic Phosphatase of Regenerating Liver-3 (PRL-3) and impact its interaction with a known binding partner, CNNM3., PMID:37220097

Datasheet

Document Download

Anti-GFP Nanobody (SAA0976).pdf

 

$ 372
Product specifications
100 μg 372 1 mg 1860

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Anti-GFP Nanobody (SAA0976) [RWK25805]
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