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Anti-EGFP Monoclonal Antibody (1A190)

Catalog #:   MGK08402 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, IHC, IF, WB
Accession: P42212
Overview

Catalog No.

MGK08402

Species reactivity

General

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Immunogen

EGFP

Tested applications

ELISA: 1:2000-1:8000, IHC: 1:50-1:500, IF, 1:50-1:500, WB: 1:1000-1:5000

Target

Green fluorescent protein, eGFP, Enhanced GFP

Concentration

1.2 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P42212

Applications

ELISA, IHC, IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

1A190

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with EGFP antibody (MGK08402) at 1 µg/ml.

    Lane 1: Recombinant Enhanced GFP Protein (YWK08401)

    Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

    Predict MW: 28 kDa
    Observed MW: 28 kDa
References

Single-particle quantification of SARS-CoV-2 virus-like particles using flow virometry., PMID:40483334

Construction and In Vitro and In Vivo Analysis of Coxsackievirus B4 Reporter Viruses: Attenuated Virulence but Highly Efficient for Antiviral Drug Screening and Evaluation., PMID:40474695

The Junction Between nsp1β and nsp2 in the Porcine Reproductive and Respiratory Syndrome Virus Genome Is a New Site for the Insertion and Expression of Foreign Genes., PMID:40431667

Characterization of the VOC Promoter That Is Active Under Low-Salinity Conditions in the Diatom Phaeodactylum tricornutum., PMID:40422775

Validation and Optimization of PURE Ribosome Display for Screening Synthetic Nanobody Libraries., PMID:40407691

Binding characterization of small protein-conjugated ssDNA aptamer to recombinant human ICAM-1., PMID:40329889

The commercial PRRSV attenuated vaccine can be a potentially effective live trivalent vaccine vector., PMID:40316839

A Novel Prototype African Swine Fever Virus DIVA (Differentiation Between Infected and Vaccinated Animals) Serological Assay Based on the Detection of Antibodies Against the pEP153R, eGFP, and p72 Proteins., PMID:40266072

Construction and characterization of a gE/gI/TK-gene-deleted recombinant pseudorabies virus variant expressing the GP5 of the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) and NADC30-like PRRSV., PMID:40180235

AAV-mediated transduction of songbird retina., PMID:40177359

Efficient production of a novel recombinant fusion protein of EIEC effector IpaD and EGFP: Biophysical characterization and functional studies., PMID:40086498

Migration of Regulatory T Cells to the Peritumor Microenvironment of Experimental Glioblastoma., PMID:40071070

A high affinity Sybody blocks Cofilin-1 binding to F-actin in vitro and in cancer cells., PMID:40064451

An LNP-mRNA vaccine protects fish against rhabdovirus infection., PMID:40031086

Cell-Division-Independent Rapid Expression of DNA Delivered with α-Synuclein-Gold Nanoparticle Conjugates., PMID:40014054

Dual targeted lipid nanoparticles for enhanced DNA delivery and transfection of breast cancer cells., PMID:39988265

Xmas-NT: A Novel Two-Color Fluorescence Micro Neutralization Test for the Detection of Neutralizing Antibodies Against Monkeypox Virus., PMID:39977402

Development of an optimized cell-based selection system for phage display libraries., PMID:39968222

Cyclophilin-CD147 interaction enables SARS-CoV-2 infection of human monocytes and their activation via Toll-like receptors 7 and 8., PMID:39963132

DNA-loaded targeted nanoparticles as a safe platform to produce exogenous proteins in tumor B cells., PMID:39911576

The combination of rAAV pseudo-lipid nanoparticle and triamcinolone acetonide enables multi-administration to liver., PMID:39897641

Modifying non-coding regions of linear DNA vaccines to explore the interplay of expression and inflammation in immunogenicity., PMID:39829233

Recombinant avian metapneumovirus subtype C expressing HA protein of H9N2 avian influenza virus are stable and induce protection., PMID:39744386

Abnormal c-Fos expression in TetTag mice containing fos-EGFP., PMID:39741565

Selective Colocalization of GHSR and GLP-1R in a Subset of Hypothalamic Neurons and Their Functional Interaction., PMID:39737802

A protocol for high-throughput screening for small chemicals promoting macrophage-mediated tumor cell phagocytosis in mice., PMID:39700010

CKAP4 in hepatocellular carcinoma: competitive RETREG1/FAM134B binding, reticulophagy regulation, and cancer progression., PMID:39689859

Low-density Lipoprotein Receptor is an important host factor in flaviviral entry and replication in neurons., PMID:39689643

Development and application of an uncapped mRNA platform., PMID:39648715

TNF-α Suppression Attenuates Limbal Stem Cell Damage in Ocular Injury., PMID:39626088

Lipid Nanoparticle-Mediated Liver-Specific Gene Therapy for Hemophilia B., PMID:39598550

Multiple pathways to evaluate the immunoprotective effect of Turkeys Herpesvirus recombinant vaccine expressing HA of H9N2., PMID:39577170

Avian TRIM13 attenuates antiviral innate immunity by targeting MAVS for autophagic degradation., PMID:39508267

Potent broadly neutralizing antibodies mediate efficient antibody-dependent phagocytosis of HIV-infected cells., PMID:39466835

Rectal Epithelial Stem Cell Kinetics in Acute Radiation Proctitis., PMID:39457033

CuBe: a geminivirus-based copper-regulated expression system suitable for post-harvest activation., PMID:39435699

Design of the conserved epitope peptide of SARS-CoV-2 spike protein as the broad-spectrum COVID-19 vaccine., PMID:39412657

PARP1 acetylation at K119 is essential in regulating the progression and proliferation of cervical cancer cells., PMID:39400626

Development and characterization of a recombinant Senecavirus A expressing enhanced green fluorescent protein., PMID:39391602

Detection of Neutralizing Antibodies in Serum Samples Using a SARS-CoV-2 Pseudotyped Virus Assay., PMID:39373132

Facile quantitative diagnostic testing for neutralizing antibodies against Chikungunya virus., PMID:39350079

Serological Markers of Clinical Improvement in MuSK Myasthenia Gravis., PMID:39250722

Resistance-based directed evolution of nanobodies for higher affinity in prokaryotes., PMID:39245149

Intravital imaging of cardiac tissue utilizing tissue-stabilized heart window chamber in live animal model., PMID:39224098

CD2AP is Co-Expressed with Tropomyosin-Related Kinase A and Ras-Related Protein Rab-5A in Cholinergic Neurons of the Murine Basal Forebrain., PMID:39211110

Immune Responses Induced by a Recombinant Lactiplantibacillus plantarum Surface-Displaying the gD Protein of Pseudorabies Virus., PMID:39205163

Inhibition of enhanced green fluorescent protein cytosolic delivery mediated by modified cell-penetrating peptide due to high overexpression of Caveolin-1., PMID:39197200

Comparison of SNCG and NEFH Promoter-Driven Expression of Human SIRT1 Expression in a Mouse Model of Glaucoma., PMID:39177995

Genome-wide CRISPR/Cas9 screen identifies SLC39A9 and PIK3C3 as crucial entry factors for Ebola virus infection., PMID:39173055

Development of a synthetic library of humanized nanobodies for targeted IL-6 inhibition., PMID:39108599

Datasheet
$ 130
Product specifications
50 μg 130 100 μg 220

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For research use only. Not for human or drug use.

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Anti-EGFP Monoclonal Antibody (1A190) [MGK08402]
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