Catalog No.
RVV14105
Species reactivity
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strainWR))
Host species
Human
Isotype
IgG1, kappa
Clonality
Monoclonal
Applications
ELISA, Neutralization
Target
Protein A27, VACWR150, A27L
Concentration
2.65 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P11258
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
203#
Note
For research use only.
Epitope detection in the envelope of intracellular naked orthopox viruses and identification of encoding genes., PMID:7513922
Inducible expression of the vaccinia virus A17L gene provides a synchronized system to monitor sorting of viral proteins during morphogenesis., PMID:8892884
Identification of functional domains in the 14-kilodalton envelope protein (A27L) of vaccinia virus., PMID:10516016
Chimeras between the human immunodeficiency virus (HIV-1) Env and vaccinia virus immunogenic proteins p14 and p39 generate in mice broadly reactive antibodies and specific activation of CD8+ T cell responses to Env., PMID:10856792
The A17L gene product of vaccinia virus is exposed on the surface of IMV., PMID:11882999
Four-gene-combination DNA vaccine protects mice against a lethal vaccinia virus challenge and elicits appropriate antibody responses in nonhuman primates., PMID:12620810
Smallpox DNA vaccine protects nonhuman primates against lethal monkeypox., PMID:15078924
Differential efficacy of vaccinia virus envelope proteins administered by DNA immunisation in protection of BALB/c mice from a lethal intranasal poxvirus challenge., PMID:15308360
Subunit recombinant vaccine protects against monkeypox., PMID:16888017
Human T-cell responses to vaccinia virus envelope proteins., PMID:17005679
MHC-peptide-specific antibodies reveal inefficient presentation of an HLA-A*0201-restricted, Melan-A-derived peptide after active intracellular processing., PMID:17559180
Specific tumor cell targeting by a recombinant MVA expressing a functional single chain antibody on the surface of intracellular mature virus (IMV) particles., PMID:18158739
Vaccination of BALB/c mice with Escherichia coli-expressed vaccinia virus proteins A27L, B5R, and D8L protects mice from lethal vaccinia virus challenge., PMID:18199639
Differential antigen requirements for protection against systemic and intranasal vaccinia virus challenges in mice., PMID:18448519
Targeting the vaccinia virus L1 protein to the cell surface enhances production of neutralizing antibodies., PMID:18485547
Molecular smallpox vaccine delivered by alphavirus replicons elicits protective immunity in mice and non-human primates., PMID:19833247
Single-shot immunization with recombinant adenovirus encoding vaccinia virus glycoprotein A27L is protective against a virulent respiratory poxvirus infection., PMID:20653083
L1R, A27L, A33R and B5R vaccinia virus genes expressed by fowlpox recombinants as putative novel orthopoxvirus vaccines., PMID:23578094
CD4+ T cells provide intermolecular help to generate robust antibody responses in vaccinia virus-vaccinated humans., PMID:23667112
Systemically administered DNA and fowlpox recombinants expressing four vaccinia virus genes although immunogenic do not protect mice against the highly pathogenic IHD-J vaccinia strain., PMID:24050999
Immunomodulator-based enhancement of anti smallpox immune responses., PMID:25875833
A Chimeric HIV-1 gp120 Fused with Vaccinia Virus 14K (A27) Protein as an HIV Immunogen., PMID:26208356
Structural analysis and immunogenicity of recombinant major envelope protein (rA27L) of buffalopox virus, a zoonotic Indian vaccinia-like virus., PMID:26319070
[A New Simple Technique for Producing Labeled Monoclonal Antibodies for Antibody Pair Screening in Sandwich-ELISA]., PMID:26615636
Protection of mice against the highly pathogenic VVIHD-J by DNA and fowlpox recombinant vaccines, administered by electroporation and intranasal routes, correlates with serum neutralizing activity., PMID:27637905
Co-administration of recombinant major envelope proteins (rA27L and rH3L) of buffalopox virus provides enhanced immunogenicity and protective efficacy in animal models., PMID:28259752
Vaccination with a codon-optimized A27L-containing plasmid decreases virus replication and dissemination after vaccinia virus challenge., PMID:28629922
Prokaryotic expression, purification and evaluation of goatpox virus ORF117 protein as a diagnostic antigen in indirect ELISA to detect goatpox., PMID:30778744
The Envelope-Based Fusion Antigen GP120C14K Forming Hexamer-Like Structures Triggers T Cell and Neutralizing Antibody Responses Against HIV-1., PMID:31867001
Proteomic assessment of humoral immune responses in smallpox vaccine recipients., PMID:34952760
Comparison of the immunogenicity and protective efficacy of ACAM2000, MVA, and vectored subunit vaccines for Mpox in rhesus macaques., PMID:38536937
Levels of antibodies against the monkeypox virus compared by HIV status and historical smallpox vaccinations: a serological study., PMID:38767199
The Generation and Characterization of Monoclonal Antibodies against the MPXV A29L Protein., PMID:39205158
Development of Smallpox Antibody Testing and Surveillance Following Smallpox Vaccination in the Republic of Korea., PMID:39460271
Monkeypox virus A29L protein as the target for specific diagnosis and serological analysis., PMID:39570405
Development and validation of recombinant A27L based indirect-ELISA for Sheeppox and Goatpox disease in India., PMID:39869249
For research use only. Not for human or drug use.
