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Anti-Human B2M/Beta-2-microglobulin Antibody (BBM.1)

Catalog #:   RHF46101 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG2b-kappa
Applications: ELISA, FCM, WB
Accession: P61769
Overview

Catalog No.

RHF46101

Species reactivity

Human

Host species

Mouse

Isotype

IgG2b-kappa

Clonality

Monoclonal

Target

Beta-2-microglobulin, Beta-2-microglobulin form pI 5.3, B2M, CDABP0092, HDCMA22P

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P61769

Applications

ELISA, FCM, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

BBM.1

Data Image
  • Bioactivity
    Detects B2M/Beta-2-microglobulin in indirect ELISAs.
  • Flow-cytometry
    Flow-cytometry using anti-human B2M antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # RHF46101 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human B2M antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # RHF46101 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human B2M antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # RHF46101 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human B2M antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # RHF46101 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human B2M antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # RHF46101 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • SEC-HPLC
    The purity of this product is >95% as determined by SEC-HPLC.
References

Intratumoral IL12 mRNA administration activates innate and adaptive pathways in checkpoint inhibitor resistant tumors resulting in complete responses., PMID:40321762

An Exosomal mRNA Urine Test for Detection and Risk Stratification of Human Kidney Transplant Rejection., PMID:40303229

Interim PET after 4 cycles predicts outcome in histomolecularly confirmed primary mediastinal B-cell lymphoma., PMID:40030008

Clinico-genomic features predict distinct metastatic phenotypes in cutaneous melanoma., PMID:39975034

A self-activated and protective module enhances the preclinical performance of allogeneic anti-CD70 CAR-T cells., PMID:39906740

Craters on the melanoma surface facilitate tumor-immune interactions and demonstrate pathologic response to checkpoint blockade in humans., PMID:39345527

Conformational Alterations of the Cell Surface of Monomeric and Dimeric β2m-Free HLA-I (Proto-HLA) May Enable Novel Immune Functions in Health and Disease., PMID:39057057

Innate and adaptive immune response of Rainbow trout (Oncorhynchus mykiss) naturally infected with Yersinia ruckeri., PMID:38960109

IFNγ mediates the resistance of tumor cells to distinct NK cell subsets., PMID:38955423

BCMA/CD47-directed universal CAR-T cells exhibit excellent antitumor activity in multiple myeloma., PMID:38783333

Prostate cancer research: tools, cell types, and molecular targets., PMID:38595814

Long-lasting mRNA-encoded interleukin-2 restores CD8+ T cell neoantigen immunity in MHC class I-deficient cancers., PMID:38490213

Differentially Expressed Genes Involved in Primary Resistance to Immunotherapy in Patients with Advanced-Stage Pulmonary Cancer., PMID:38396726

Diversity in the HLA-I Recognition of HLA-F Monoclonal Antibodies: HLA-F or HLA-Ib Monospecific, HLA-E or HLA-G Bispecific Antibodies with or without HLA-Ia Reactivity., PMID:38390869

Mechanism exploration and biomarker identification of glycemic deterioration in patients with diseases of the exocrine pancreas., PMID:38388766

Allogeneic CAR-T cells with of HLA-A/B and TRAC disruption exhibit promising antitumor capacity against B cell malignancies., PMID:38231412

Generation and optimization of off-the-shelf immunotherapeutics targeting TCR-Vβ2+ T cell malignancy., PMID:38225288

A targeted antibody-based array reveals a serum protein signature as biomarker for adolescent idiopathic scoliosis patients., PMID:37667193

Concurrent predictors of an immune responsive tumor microenvironment within tumor mutational burden-high breast cancer., PMID:37637072

Cell Surface B2m-Free Human Leukocyte Antigen (HLA) Monomers and Dimers: Are They Neo-HLA Class and Proto-HLA?, PMID:37627243

How I Manage Chronic Lymphocytic Leukemia., PMID:37606492

Immune Marker Spatial Distribution and Clinical Outcome after PD-1 Blockade in Mismatch Repair-deficient, Advanced Colorectal Carcinomas., PMID:37566222

Conversion of senescent cartilage into a pro-chondrogenic microenvironment with antibody-functionalized copper sulfate nanoparticles for efficient osteoarthritis therapy., PMID:37550685

Efficacy and safety of PD-1 monoclonal antibody plus rituximab in relapsed/refractory diffuse large B cell lymphoma patients., PMID:37294015

β2-Microglobulin coaggregates with Aβ and contributes to amyloid pathology and cognitive deficits in Alzheimer's disease model mice., PMID:37264159

Primary Mediastinal B-Cell Lymphoma in Children and Young Adults., PMID:36898366

β2-microglobulin functions as an endogenous NMDAR antagonist to impair synaptic function., PMID:36868208

Allele mining in gene encoding fc fragment of IgG receptor, transporter, alpha (FCGRT) and association of nucleotide variants with passive transfer of immunity in neonatal buffalo calves., PMID:36757548

A Comprehensive Biomarker Analysis of Microsatellite Unstable/Mismatch Repair Deficient Colorectal Cancer Cohort Treated with Immunotherapy., PMID:36613564

Protection of cell therapeutics from antibody-mediated killing by CD64 overexpression., PMID:36593395

Risk Factors for and Outcomes of Follicular Lymphoma Histological Transformation at First Progression in the GALLIUM Study., PMID:36379880

Isotope-dilution liquid chromatography-tandem mass spectrometry method for serum beta 2-microglobulin quantification., PMID:36228451

Identification of immune cell infiltration and diagnostic biomarkers in unstable atherosclerotic plaques by integrated bioinformatics analysis and machine learning., PMID:36211422

[Enhanced Natural Killers with CISH and B2M Gene Knockouts Reveal Increased Cytotoxicity in Glioblastoma Primary Cultures]., PMID:36165021

Identifying stable reference genes in polyethene glycol precipitated urinary extracellular vesicles for RT-qPCR-based gene expression studies in renal graft dysfunction patients., PMID:36122652

A novel immune-related epigenetic signature based on the transcriptome for predicting the prognosis and therapeutic response of patients with diffuse large B-cell lymphoma., PMID:36055572

Donor natural killer cells trigger production of β-2-microglobulin to enhance post-bone marrow transplant immunity., PMID:35984965

Long follow-up of symptomatic Multiple Myeloma patients after Covid-19 vaccination (BNT162b2): A single-institution retrospective experience., PMID:35966626

β2-Microglobulin Maintains Glioblastoma Stem Cells and Induces M2-like Polarization of Tumor-Associated Macrophages., PMID:35841593

Chitosan oligosaccharide improves the mucosal immunity of small intestine through activating SIgA production in mice: Proteomic analysis., PMID:35569308

Transcriptomic analysis of the innate immune signatures of a SARS-CoV-2 protein subunit vaccine ZF2001 and an mRNA vaccine RRV., PMID:35343384

Urinary detection of early responses to checkpoint blockade and of resistance to it via protease-cleaved antibody-conjugated sensors., PMID:35241815

CRISPR/Cas9-Engineered HLA-Deleted Glomerular Endothelial Cells as a Tool to Predict Pathogenic Non-HLA Antibodies in Kidney Transplant Recipients., PMID:35167486

Detection of nucleotide variants in FCGRT (Fc fragment of IgG, receptor, transporter, alpha) gene and their influence on colostral IgG concentration in Indian water buffalo (Bubalus bubalis)., PMID:35152368

Partial immunoparesis contributes to risk of early infections in patients with multiple myeloma., PMID:35116375

Human IgE does not bind to human FcRn., PMID:34996950

Inhibition of FGFR Reactivates IFNγ Signaling in Tumor Cells to Enhance the Combined Antitumor Activity of Lenvatinib with Anti-PD-1 Antibodies., PMID:34753772

Pathological review of cardiac amyloidosis using autopsy cases in a single Japanese institution., PMID:34653913

Targeted clearance of senescent cells using an antibody-drug conjugate against a specific membrane marker., PMID:34645909

Pan-cancer analysis of longitudinal metastatic tumors reveals genomic alterations and immune landscape dynamics associated with pembrolizumab sensitivity., PMID:34446728

Datasheet
$ 328
Product specifications
100 μg 328 1 mg 1640

Contact Information

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Mail: support@antibodysystem.com

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For research use only. Not for human or drug use.

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Anti-Human B2M/Beta-2-microglobulin Antibody (BBM.1) [RHF46101]
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