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Anti-Human Gal-9/LGALS9 Antibody (G9.2-17)

Catalog #:   RHA04301 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG2a, kappa
Applications: Blocking, ELISA, FCM
Accession: O00182
Overview

Catalog No.

RHA04301

Species reactivity

Human

Host species

Mouse

Isotype

IgG2a, kappa

Clonality

Monoclonal

Target

Ecalectin, LGALS9, Galectin-9, Gal-9, Tumor antigen HOM-HD-21

Concentration

0.5 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O00182

Applications

Blocking, ELISA, FCM

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

G9.2-17

Data Image
  • Flow-cytometry
    Flow-cytometry using anti-human Galectin-9 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-9 antibody monoclonal antibody (Catalog # RHA04301 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human Galectin-9 antibody.MOLT-4 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-9 antibody monoclonal antibody (Catalog # RHA04301 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human Galectin-9 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-9 antibody monoclonal antibody (Catalog # RHA04301 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
References

Galectin 9 rescues the inducibility of IL-10 expression in regulatory B cells of patients with food allergy., PMID:39747510

Immunomodulation by galectin-9: Distinct role in T cell populations, current therapeutic avenues and future potential., PMID:39571310

Cell-autonomous targeting of arabinogalactan by host immune factors inhibits mycobacterial growth., PMID:39495223

Monocyte-derived Galectin-9 and PD-L1 differentially impair adaptive and innate immune response in chronic HBV infection and their expression remain unaltered after antiviral therapy., PMID:39445017

[Research progress on T-cell immunoglobulin mucin 3/galectin 9 (TIM-3/galectin-9) in tumour immunity]., PMID:39215674

Enhanced expression of galectin-9 in triple negative breast cancer cells following radiotherapy: Implications for targeted therapy., PMID:39077999

In chronic spontaneous urticaria, increased Galectin-9 expression on basophils and eosinophils is linked to high disease activity, endotype-specific markers, and response to omalizumab treatment., PMID:39021347

Expression of Intracellular Galectin-8 and -9 in Endometrial Cancer., PMID:39000016

Chemotherapy-induced intestinal epithelial damage directly promotes galectin-9-driven modulation of T cell behavior., PMID:38883813

Establishment of novel anti-TIM-3 antibodies interfering with its binding to ligands., PMID:38560237

The balance between CD4+ T helper 17 and T-cell immunoglobulin and mucin domain 3 is involved in the pathogenesis and development of atrial fibrillation., PMID:38357157

Inhibition of Galectin-9 sensitizes tumors to anthracycline treatment via inducing antitumor immunity., PMID:37781042

TIM-3 as a promising target for cancer immunotherapy in a wide range of tumors., PMID:37567938

A spatially resolved timeline of the human maternal-fetal interface., PMID:37468587

Gal-9/Tim-3 signaling pathway activation suppresses the generation of Th17 cells and promotes the induction of Foxp3+ regulatory T cells in renal ischemia-reperfusion injury., PMID:36921488

Mesenchymal stem cells protect against sepsis-associated acute kidney injury by inducing Gal-9/Tim-3 to remodel immune homeostasis., PMID:36883358

Galectin-9 blockade synergizes with ATM inhibition to induce potent anti-tumor immunity., PMID:36778120

Increased Galectin-9 Levels Correlate with Disease Activity in Patients with DMARD-Naïve Rheumatoid Arthritis and Modulate the Secretion of MCP-1 and IL-6 from Synovial Fibroblasts., PMID:36672263

Plasma galectins and metabolites in advanced head and neck carcinomas: evidence of distinct immune characteristics linked to hypopharyngeal tumors., PMID:36545254

Contribution of the TIM-3/Gal-9 immune checkpoint to tropical parasitic diseases., PMID:36509129

Galectin-9 contributes to the pathogenesis of atopic dermatitis via T cell immunoglobulin mucin-3., PMID:35967337

Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive pulmonary disease severity: An observational study., PMID:35687771

Development and characterization of anti-galectin-9 antibodies that protect T cells from galectin-9-induced cell death., PMID:35283189

Obesity-induced galectin-9 is a therapeutic target in B-cell acute lymphoblastic leukemia., PMID:35241678

Galectin-9 Triggers Neutrophil-Mediated Anticancer Immunity., PMID:35052746

Modulation of the Gal-9/TIM-3 Immune Checkpoint with α-Lactose. Does Anomery of Lactose Matter?, PMID:34944985

Galectin-9 mediates neutrophil capture and adhesion in a CD44 and β2 integrin-dependent manner., PMID:34847625

Association between inflammatory cytokines and immune-checkpoint molecule in rheumatoid arthritis., PMID:34793561

Differences in Immune Checkpoints Expression (TIM-3 and PD-1) on T Cells in Women with Recurrent Miscarriages-Preliminary Studies., PMID:34575293

Unbiased Analysis of Temporal Changes in Immune Serum Markers in Acute COVID-19 Infection With Emphasis on Organ Failure, Anti-Viral Treatment, and Demographic Characteristics., PMID:34177897

Chemoradiation induces upregulation of immunogenic cell death-related molecules together with increased expression of PD-L1 and galectin-9 in gastric cancer., PMID:34112882

THE ROLE OF GALECTIN-9 IN PATIENTS WITH CHRONIC VIRAL HEPATITIS C AND ITS CONNECTION WITH THE TYPE OF THERAPY, THE DEGREE OF FIBROSIS, CLINICAL, LABORATORY, AUTOIMMUNE AND INTEGRATIVE INDICATORS., PMID:34090287

High Levels of the Cleaved Form of Galectin-9 and Osteopontin in the Plasma Are Associated with Inflammatory Markers That Reflect the Severity of COVID-19 Pneumonia., PMID:34067072

Serum checkpoint molecules in patients with IgG4-related disease (IgG4-RD)., PMID:34030721

Serum galectin-9 could be a potential biomarker in assessing the disease activity of antineutrophil cytoplasmic antibody-associated vasculitis., PMID:34001301

Expression and Prognostic Value of the Immune Checkpoints Galectin-9 and PD-L1 in Glioblastomas., PMID:33990845

The landscape of immune checkpoints expression in non-small cell lung cancer: a narrative review., PMID:33718041

Serial transplantation unmasks galectin-9 contribution to tumor immune escape in the MB49 murine model., PMID:33664349

Galectin-9 interacts with PD-1 and TIM-3 to regulate T cell death and is a target for cancer immunotherapy., PMID:33547304

Oridonin Could Inhibit Inflammation and T-cell Immunoglobulin and Mucin-3/Galectin-9 (TIM-3/Gal-9) Autocrine Loop in the Acute Myeloid Leukemia Cell Line (U937) as Compared to Doxorubicin., PMID:33463129

Effect of the use of Galectin-9 and blockade of TIM-3 receptor in the latent cellular reservoir of HIV-1., PMID:33361434

Galectin-9 regulates follicular helper T cells to inhibit humoral autoimmunity-induced pulmonary fibrosis., PMID:33316546

Galectin-9 expression defines exhausted T cells and impaired cytotoxic NK cells in patients with virus-associated solid tumors., PMID:33310773

Galectin-9 bridges human B cells to vascular endothelium while programming regulatory pathways., PMID:33285511

Galectin-9 is required for endometrial regenerative cells to induce long-term cardiac allograft survival in mice., PMID:33153471

Selective Upregulation of CTLA-4 on CD8+ T Cells Restricted by HLA-B*35Px Renders them to an Exhausted Phenotype in HIV-1 infection., PMID:32760139

Increased serum levels of galectin-9 in patients with chikungunya fever., PMID:32565125

Galectin-9 expression as a poor prognostic factor in patients with renal cell carcinoma., PMID:32424467

Identification and characterization of M6903, an antagonistic anti-TIM-3 monoclonal antibody., PMID:32313722

Differential regulation and correlation between galectin-9 and anti-CCP antibody (ACPA) in rheumatoid arthritis patients., PMID:32293530

Datasheet
$ 372
Product specifications
100 μg 372 1 mg 1860

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For research use only. Not for human or drug use.

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Anti-Human Gal-9/LGALS9 Antibody (G9.2-17) [RHA04301]
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