Anti-ROCK2 Polyclonal Antibody

Catalog No.

PHB09101

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ROCK2 (Glu907-Gly1140).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

ROCK2,KIAA0619,Rho-associated,coiled-coil-containing protein kinase 2,p164 ROCK-2,ROCK-II,Rho kinase 2,Rho-associated,coiled-coil-containing protein kinase II,Rho-associated protein kinase 2

Purification

Purified by antigen affinity column.

Accession

O75116

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

In Vitro Experiment Present ROCK2 Inhibition Promotes the Therapeutic Effect of Bevacizumab in the Treatment of Glioblastoma Multiforme., PMID:39792535

The ROCK-1/2 inhibitor RKI-1447 blocks N-MYC, promotes cell death, and emerges as a synergistic partner for BET inhibitors in neuroblastoma., PMID:39307412

Augmentation of functional recovery via ROCK/PI3K/AKT pathway by Fasudil Hydrochloride in a rat sciatic nerve transection model., PMID:39007038

Modulating asthma-COPD overlap responses with IL-17 inhibition., PMID:37965351

Philadelphia chromosome-negative myeloproliferative chronic neoplasms: is clonal hematopoiesis the main determinant of autoimmune and cardio-vascular manifestations?, PMID:37915324

Involvement of Paired Immunoglobulin-Like Receptor B in Cognitive Dysfunction Through Hippocampal-Dependent Synaptic Plasticity Impairments in Mice Subjected to Chronic Sleep Restriction., PMID:36417104

Inhibition of Nogo-A rescues synaptic plasticity and associativity in APP/PS1 animal model of Alzheimer's disease., PMID:35431138

ROCK2 inhibition attenuates profibrogenic immune cell function to reverse thioacetamide-induced liver fibrosis., PMID:34917911

Critical regulation of follicular helper T cell differentiation and function by Gα13 signaling., PMID:34663730

Silencing ROCK1 ameliorates ventilator-induced lung injury in mice by inhibiting macrophages' NLRP3 signaling., PMID:34619496

Activin A and Cell-Surface GRP78 Are Novel Targetable RhoA Activators for Diabetic Kidney Disease., PMID:33799579

Effect of anti-IL17 and/or Rho-kinase inhibitor treatments on vascular remodeling induced by chronic allergic pulmonary inflammation., PMID:33357114

Profilin 1 knockdown prevents ischemic brain damage by promoting M2 microglial polarization associated with the RhoA/ROCK pathway., PMID:32291804

Claudin-5 Redistribution Induced by Inflammation Leads to Anti-VEGF-Resistant Diabetic Macular Edema., PMID:32139595

Fibronectin regulates the self-renewal of rabbit limbal epithelial stem cells by stimulating the Wnt11/Fzd7/ROCK non-canonical Wnt pathway., PMID:31150636

Immunoglobulin Binding Protein 1 as a Potential Urine Biomarker in Patients with Lupus Nephritis., PMID:31137925

Loss of ARHGEF1 causes a human primary antibody deficiency., PMID:30521495

Effect of Anti-IL17 Antibody Treatment Alone and in Combination With Rho-Kinase Inhibitor in a Murine Model of Asthma., PMID:30233389

The CCM1-CCM2 complex controls complementary functions of ROCK1 and ROCK2 that are required for endothelial integrity., PMID:30030370

Toll‑Like receptor 4 promotes the phosphorylation of CRMP2 via the activation of Rho‑kinase in MCAO rats., PMID:29749502

Discovery of Potent and Selective MRCK Inhibitors with Therapeutic Effect on Skin Cancer., PMID:29382705

ROCK-1 mediates diabetes-induced retinal pigment epithelial and endothelial cell blebbing: Contribution to diabetic retinopathy., PMID:28821742

Genetic Interference With Endothelial PPAR-γ (Peroxisome Proliferator-Activated Receptor-γ) Augments Effects of Angiotensin II While Impairing Responses to Angiotensin 1-7., PMID:28674038

Cytokine mediators of chronic graft-versus-host disease., PMID:28665299

Rho-associated protein kinase 2 (ROCK2): a new target of autoimmunity in paraneoplastic encephalitis., PMID:28554330

Inhibition of lysophosphatidic acid receptor ameliorates Sjögren's syndrome in NOD mice., PMID:28460477

The Role of Rho/Rho-Kinase Pathway in the Pathogenesis of Cholesteatoma., PMID:28288476

RhoA/ROCK pathway inhibition by fasudil suppresses the vasculogenic mimicry of U2OS osteosarcoma cells in vitro., PMID:28225457

Targeting ROCK2 rather than ROCK1 inhibits Ewing sarcoma malignancy., PMID:28112365

ROCK2 signaling is required to induce a subset of T follicular helper cells through opposing effects on STATs in autoimmune settings., PMID:27436361

Attenuation of obesity-induced insulin resistance in mice with heterozygous deletion of ROCK2., PMID:27163743

Targeted Rho-associated kinase 2 inhibition suppresses murine and human chronic GVHD through a Stat3-dependent mechanism., PMID:26983850

ROCK inhibition with fasudil promotes early functional recovery of spinal cord injury in rats by enhancing microglia phagocytosis., PMID:26838736

In vivo expression and regulation of genes associated with vascularization during early response of sutures to tensile force., PMID:26825658

Immunohistochemical evaluation of ROCK activation in invasive breast cancer., PMID:26626121

Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis., PMID:26536659

Examination of segmental average mass spectra from liquid chromatography-tandem mass spectrometric (LC-MS/MS) data enables screening of multiple types of protein modifications., PMID:26388481

Rotenone inhibits primary murine myotube formation via Raf-1 and ROCK2., PMID:25827955

Functional relationship among PLK2, PLK4 and ROCK2 to induce centrosome amplification., PMID:25590559

In vivo screening for substrates of protein kinase A using a combination of proteomic approaches and pharmacological modulation of kinase activity., PMID:25399539

Abrogation of airway hyperresponsiveness but not inflammation by rho kinase insufficiency., PMID:25323425

Upregulation of Gem relates to retinal ganglion cells apoptosis after optic nerve crush in adult rats., PMID:24948002

Prostaglandin E(2)-dependent blockade of actomyosin and stress fibre formation is mediated through S1379 phosphorylation of ROCK2., PMID:24610576

Ser1333 phosphorylation indicates ROCKI activation., PMID:24168723

Anti-β2GPI antibodies stimulate endothelial cell microparticle release via a nonmuscle myosin II motor protein-dependent pathway., PMID:23954892

CD99 suppresses osteosarcoma cell migration through inhibition of ROCK2 activity., PMID:23644663

Distinct distribution and localization of Rho-kinase in mouse epithelial, muscle and neural tissues., PMID:22986902

The effects of erythropoietin on RhoA/Rho-associated kinase expression in rat retinal explants cultured with glutamate., PMID:22711191

Elevated levels of RhoA in the optic nerve head of human eyes with glaucoma., PMID:22495072

In vivo evidence that TRAF4 is required for central nervous system myelin homeostasis., PMID:22363515

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