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Anti-PPL Polyclonal Antibody

Catalog #:   PHA91001 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: O60437
Overview

Catalog No.

PHA91001

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PPL (Glu1439-Gly1716).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

190 kDa paraneoplastic pemphigus antigen,Periplakin,195 kDa cornified envelope precursor protein,KIAA0568,PPL

Purification

Purified by antigen affinity column.

Accession

O60437

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with PPL antibody (PHA91001) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 35 kDa
    Observed MW: 35 kDa
  • Immunocytochemistry/ Immunofluorescence
    PPL in HaCat Cell Line.
    The HaCat cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with PPL (PHA91001) at 7.6 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

Increasing the developability of lambda chain antibodies: engineering to confer protein L binding activity and its consequences., PMID:40368210

Effect of alirocumab on postprandial hyperlipidaemia in patients with type 2 diabetes: A randomized, double-blind, placebo-controlled, cross-over trial., PMID:40045751

[Primary Pancreatic Lymphoma Coexisting with Intraductal Papillary Mucinous Neoplasm-A Case Report]., PMID:39949016

A broad spectrum Shigella vaccine based on VirG53-353 multiepitope region produced in a cell-free system., PMID:39805874

Long-term efficacy and safety of danicopan as add-on therapy to ravulizumab or eculizumab in PNH with significant EVH., PMID:39700502

Efficacy and safety of faricimab for neovascular age-related macular degeneration: a systematic review and network meta-analysis., PMID:39043575

Cabozantinib and nivolumab with or without live bacterial supplementation in metastatic renal cell carcinoma: a randomized phase 1 trial., PMID:38942995

PD-L1 targeted peptide demonstrates potent antitumor and immunomodulatory activity in cancer immunotherapy., PMID:38745661

Primary pancreatic peripheral T-cell lymphoma: A case report., PMID:38660638

Identifying the in vivo-induced antigenic genes is a strategy to develop DNA vaccine against Nocardia seriolae in hybrid snakehead (Channa maculata ♀ × Channa argus ♂)., PMID:38309489

Development of Prodrug-Payloads for Targeted Therapeutic Applications of Platinum-Acridine Anticancer Agents., PMID:37813818

Recruitment into antibody prevalence studies: a randomized trial of postcards vs. letters as invitations., PMID:37481522

A sensitive tissue factor activity assay determined by an optimized thrombin generation method., PMID:37467256

Autoantibodies are associated with disease progression in idiopathic pulmonary fibrosis., PMID:37080573

A Novel Shigella O-Polysaccharide-IpaB Conjugate Vaccine Elicits Robust Antibody Responses and Confers Protection against Multiple Shigella Serotypes., PMID:37017547

Insulin-like growth factor-binding protein-7 (IGFBP7) links senescence to heart failure., PMID:39196168

Recombinant Protein L: Production, Purification and Characterization of a Universal Binding Ligand., PMID:36206851

Changes in glycans on platelet microparticles released during storage of apheresis platelets are associated with phosphatidylserine externalization and phagocytosis., PMID:35467785

Variable-heavy (VH) families influencing IgA1&2 engagement to the antigen, FcαRI and superantigen proteins G, A, and L., PMID:35444201

B cell receptor signaling strength modulates cancer immunity., PMID:35289309

More Than Meets the Kappa for Antibody Superantigen Protein L (PpL)., PMID:35225872

Distinguishing paraneoplastic pemphigus with keratotic skin lesions lacking anti-plakin antibodies from severe lichen planus., PMID:33871365

Week 2 Symptomatic Response with Vedolizumab as a Predictive Factor in Japanese Anti-TNFα-Naive Patients with Ulcerative Colitis: A post hoc Analysis of a Randomized, Placebo-Controlled Phase 3 Trial., PMID:33454706

Primary pulmonary diffuse large B-cell lymphoma with multiple ground-glass nodules as the primary manifestation: A case report., PMID:33327289

Circulating Rather Than Intestinal PCSK9 (Proprotein Convertase Subtilisin Kexin Type 9) Regulates Postprandial Lipemia in Mice., PMID:32673528

Minimal agreement between basophil activation test and immunoassay in diagnosis of penicillin allergy., PMID:32532468

Exploration and Modulation of Antibody Fragment Biophysical Properties by Replacing the Framework Region Sequences., PMID:32326443

The Science Underlying COVID-19: Implications for the Cardiovascular System., PMID:32293910

Prospective and comparative study of paroxysmal nocturnal hemoglobinuria patients treated or not by eculizumab: Focus on platelet extracellular vesicles., PMID:31277120

Optimization of Zika virus envelope protein production for ELISA and correlation of antibody titers with virus neutralization in Mexican patients from an arbovirus endemic region., PMID:30587198

Penicillin minor determinants: History and relevance for current diagnosis., PMID:30248407

Postprandial Increase in Blood Plasma Levels of Tissue Factor-Bearing (and Other) Microvesicles Measured by Flow Cytometry: Fact or Artifact?, PMID:31249938

Molecular Modeling of the Interaction of Protein L with Antibodies., PMID:31457247

Persistent microglial activation and synaptic loss with behavioral abnormalities in mouse offspring exposed to CASPR2-antibodies in utero., PMID:28755208

Eculizumab decreases the procoagulant activity of extracellular vesicles in paroxysmal nocturnal hemoglobinuria: A pilot prospective longitudinal clinical study., PMID:28646725

Utility of basophil activation testing to assess perioperative anaphylactic reactions in real-world practice., PMID:28580612

Clickable Antifouling Polymer Brushes for Polymer Pen Lithography., PMID:28296390

Effect of a cocoa diet on the small intestine and gut-associated lymphoid tissue composition in an oral sensitization model in rats., PMID:28189917

The fingerprint of antimitochondrial antibodies and the etiology of primary biliary cholangitis., PMID:28100006

Perip7lakin is a target for autoimmunity in asthma., PMID:27717390

A B-Cell Superantigen Induces the Apoptosis of Murine and Human Malignant B Cells., PMID:27603942

Click-Chemistry Based Allergen Arrays Generated by Polymer Pen Lithography for Mast Cell Activation Studies., PMID:27511293

Extremities of the N-terminus of envoplakin and C-terminus of its linker subdomain are major epitopes of paraneoplastic pemphigus., PMID:27427435

Expression of Human Immunodeficiency Virus Type 1 Neutralizing Antibody Fragments Using Human Vaginal Lactobacillus., PMID:26950606

A method to confer Protein L binding ability to any antibody fragment., PMID:26683650

Ranibizumab Plus Combined Surgery for Treatment of Neovascular Glaucoma with Vitreous Hemorrhage., PMID:26228222

Origin and levels of circulating microparticles in normal pregnancy: A longitudinal observation in healthy women., PMID:26067611

Robust vaginal colonization of macaques with a novel vaginally disintegrating tablet containing a live biotherapeutic product to prevent HIV infection in women., PMID:25875100

Treatment of murine mast cells with IgEκ and protein L enhances apoptotic cell death induced by IL-3 withdrawal., PMID:25522877

Serum periplakin as a potential biomarker for urothelial carcinoma of the urinary bladder., PMID:25520130

Datasheet

Document Download

Anti-PPL Polyclonal Antibody.pdf

 

$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-PPL Polyclonal Antibody [PHA91001]
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