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Anti-Human Fibrin Antibody (59D8)

Catalog #:   RHB99201 Specific References (57) DATASHEET
Host species: Mouse
Isotype: IgG1, kappa
Applications: IF, IHC, RIA, WB
Accession: P02675
Overview

Catalog No.

RHB99201

Species reactivity

Human

Host species

Mouse

Isotype

IgG1, kappa

Clonality

Monoclonal

Applications

IF, IHC, RIA, WB

Target

FGB, Fibrinogen beta chain, Fibrin

Concentration

3.02 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P02675

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

59D8

Note

For research use only.

Data Image
  • SEC-HPLC
    The purity of this product is >95% as determined by SEC-HPLC.
  • Immunofluorescence
    Wicked distance is viscosity dependent based on platelet-fibrin interactions. a Maximum projection confocal images of the entire RAT test strips (scale bar = 2000 μm) blank (top left), antibody characterisation (ABC, top right), 0 mM (bottom left) and 100 mM (bottom right) CaCl2 recalcified whole blood sample. Platelets (cyan, first row), fibrin (magenta, second row) and merged (last row). Bar charts representing the mean fluorescent intensity for 8-bit images sampled at random ROI across the entire RAT test strip b Platelets and c Fibrin (n = 4, ns = not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001, assessed by one-way ANOVA.
    Data sourced from citation (PMID: DOI: 10.1007/s44258-024-00025-3)
  • Immunofluorescence
    Tie2-Cre/TMX4fl/fl mice exhibit a decrease in platelet accumulation and fibrin deposition. (A) Platelets and fibrin were detected using Alexa Fluor 488-conjugated anti-CD41 F(ab)2 antibody (green) and Alexa Fluor 647-conjugated anti-fibrin antibody (red), respectively. Representative fluorescence images from intravital microscopy show platelet accumulation (green) and fibrin deposition (red). Original magnification x64. Scale bars, 30 μm. (B, C) The mean ± SEM of fluorescence intensities (FIs) of anti-CD41 antibody (B) and anti-fibrin antibody(C) with the area under the curve were analyzed using a Mann-Whitney rank-sum test. ***P < .001. The data were obtained using 30 thrombi from 3 mice for each experimental condition.
    Data sourced from citation (PMID: 39307246)
  • Immunofluorescence
    Figure 2. Localization of tissue-type plasminogen activator in acute ischemic stroke thrombi. A. Representative images of immunofluorescent localization of tissue-type plasminogen activator (tPA, green) in thrombi from IS patients. Top panels: accumulation of tPA in association with compact fibrin (red) in the thrombus outer shell (arrows). Middle panels: tPA bound to fibrin fibers in a red blood cell-rich area of the thrombus core and with compact fibrin (arrows). Lower panels: the white arrows indicate tPA associated with clusters of dense fibrin entangled with extracellular DNA (cyan) close to the thrombus surface. The images shown were acquired in a thrombus from a patient having received intravenous thrombolysis prior to EVT and are representative of 10 different thrombi.
    Data sourced from citation (DOI: DOI: https://doi.org/10.1101/2025.03.28.646062)
  • Bioactivity
    ERp29 deficiency potentiates platelet accumulation and fibrin deposition and venous thrombosis. (A) Representative images of platelet accumulation (Green) and fibrin generation (Red), as visualized by Dylight-488-conjugated anti-CD42c and Alexa-647-conjugated fibrin (59D8) antibody, respectively, at indicated time points following laser injury in the cremaster arterioles in ERp29fl/fl and Pf4-Cre/ERp29fl/fl mice. Scale bar, 30 μm. The median fluorescence intensity of platelets
    Data sourced from citation (PMID: 40336049)
  • Immunofluorescence
    Figure 3 Localization of plasminogen in acute ischemic stroke thrombi. Representative images of immunofluorescent localization of plasminogen (green) in various thrombi from acute ischemic stroke patients showing the association of plasminogen with dense fibrin (red), nucleated cells and NETs (cyan, arrows).
    Data sourced from citation (PMID: DOI: https://doi.org/10.1101/2025.03.28.646062)
  • Immunofluorescence
    B. Representative images of α2-antiplasmin (red) localization relative to fibrin (green, arrows) and NETs (cyan, asterisks). Higher magnification views of the squared areas are shown in the insets.
    Data sourced from citation (PMID: DOI: https://doi.org/10.1101/2025.03.28.646062)
References

Luminespib and AZ5104 are effective antithrombotic drugs via targeting the platelet Ero1α-PDI pathway. [RHB99201]

An engineered hirudin-albumin prodrug enables thrombus-targeted long-acting anticoagulation with reduced bleeding risk. [RHB99201]

A novel role for thioredoxin-related transmembrane protein TMX4 in platelet activation and thrombus formation. [RHB99201]

Splenic cyst deroofing complicated with B lymphoma., PMID:39232740

Fibrinogenolytic potential of venoms of medically important Brazilian snakes., PMID:39233288

A single-domain antibody targeting factor XII inhibits both thrombosis and inflammation., PMID:39266545

The potential role of dyslipidemia in COVID-19 severity among Iraqi patients., PMID:39269829

A smartphone-based multichannel magnetoelastic immunosensor for acute aortic dissection supplementary diagnosis., PMID:39305762

Development and Epitope Mapping of Seven Mouse Anti-Human Coagulation Factor XIII-B Subunit Monoclonal Antibodies., PMID:39320988

Hypochloremia: A Potential Indicator of Poor Outcomes in COVID-19., PMID:39336455

Interfering antibodies may contribute to elevated D-dimer: a case report., PMID:39342404

Autologous platelet concentrates as adjuvant in the surgical management of medication-related osteonecrosis of the jaw., PMID:39345044

Fibrinogen induces inflammatory responses via the immune activating receptor LILRA2., PMID:39376567

Encephalomyelitis and serositis caused by Chlamydia pecorum in buffalo calves from Brazil., PMID:39389928

Novel Chimeric Peptides Based on the Enolase Peptide Antigen (CEP-1) Bearing Three Post-Translational Modifications (Citrullination, Homocitrullination and Acetylation) for Determining the Diagnosis and Severity of Rheumatoid Arthritis., PMID:39408982

Risk Factors for Renal Involvement in Childhood Henoch-Schonlein Purpura (IgA Vasculitis)., PMID:39410700

The Effect of Tocilizumab on the outcome of patients with severe COVID-19 with cytokine storm syndrome in a sample of Iraqi patients., PMID:39434284

Eosinophilic cationic protein and D-Dimer are potential biomarkers to predict response to antihistamines but not to omalizumab in chronic spontaneous urticaria., PMID:39467078

Concizumab prophylaxis in people with haemophilia A or haemophilia B without inhibitors (explorer8): a prospective, multicentre, open-label, randomised, phase 3a trial., PMID:39521008

Successful treatment for a patient with antiphospholipid syndrome and decreased protein S activity exacerbated by heparin-induced thrombocytopenia: A case report., PMID:39533629

Non-bacterial thrombotic endocarditis: a clinical and pathophysiological reappraisal., PMID:39565324

Anti-Modified Peptide Antibodies (AMPAs) in Rheumatoid Arthritis: Study of the Diagnostic Value of Citrullinated, Homocitrullinated, and Acetylated Fibrin/Filaggrin Chimeric Peptides., PMID:39594151

Prognostic significance of serum interleukin-6 in severe/critical COVID-19 patients treated with tocilizumab: a detailed observational study analysis., PMID:39609511

Rapid prediction of acute thrombosis via nanoengineered immunosensors with unsupervised clustering for multiple circulating biomarkers., PMID:39661669

The sequence 581Ser-610Val in the fibrinogen Aα chain is responsible for the formation of complexes between plasminogen and αC-regions of fibrin(ogen)., PMID:39687172

Impact of antiphospholipid syndrome on placenta and uterine NK cell function: insights from a mouse model., PMID:39732740

Inhibiting Tyrosine Kinase 2 Ameliorates Antiphospholipid Syndrome Nephropathy., PMID:39742289

Bullous pemphigoid and hypercoagulability: a review., PMID:39772971

Ultrasensitive and high selectivity detection of fibrin using Y-shaped DNA-homing peptide doped probe on localized surface plasmon resonance platform., PMID:39788687

Old and New Biomarkers in Idiopathic Recurrent Acute Pericarditis (IRAP): Prognosis and Outcomes., PMID:39798014

Protection of β2GPI-deficient mice from thrombosis reflects a defect in PAR3-facilitated platelet activation., PMID:39869832

Clinicopathological analysis of anti-VEGF drug-associated renal thrombotic microangiopathy: A case series and review of the literature., PMID:39879682

Granulomatosis with Polyangiitis Initially Presenting as Frequent Urination Mimicking a Prostatic Abscess: A Case Report., PMID:39891392

Exploring Biomarkers to Predict Thrombogenic Risk in Pregnancy., PMID:39941603

Intravenous immunoglobulin prevents thrombosis in an endothelialized disease model of heparin-induced thrombocytopenia., PMID:39984158

Transcription factor RUNX1 regulates coagulation factor XIII-A (F13A1): decreased platelet-megakaryocyte F13A1 expression and clot contraction in RUNX1 haplodeficiency., PMID:39995753

Endothelial cell activation enhances thromboinflammation in vaccine-induced immune thrombotic thrombocytopenia., PMID:40085945

SARS-CoV-2 Placentitis: A Review of Pathologic Findings and Discussion of Differential Diagnosis., PMID:40090361

Post-transfusion activation of coagulation pathways during severe COVID-19 correlates with COVID-19 convalescent plasma antibody profiles., PMID:40091845

Safety and Efficacy of Long-Term Treatment of Type 1 Plasminogen Deficient Patients With Intravenous Plasminogen Replacement Therapy., PMID:40099460

Atypical presentation of H1N1-induced thrombotic microangiopathy with CD46 gene mutation
., PMID:40115863

Surface-bound FXIII enhances deposition and straightness of fibrin fibers., PMID:40139391

Pseudothrombocytopenia and other conditions associated with spuriously low platelet counts., PMID:40145275

Five decades of successive establishment of hemophilia care in Thailand., PMID:40159660

MAPK Signaling and Angiopoietin-2 Contribute to Endothelial Permeability in Capillary Malformations., PMID:40235979

Thrombotic Diathesis Combined With Connective Tissue Disease in a Chinese Teenage Male: A Case Report., PMID:40269468

Bite First, Bleed Later: How Philippine Trimeresurus Pit Viper Venoms Hijack Blood Clotting., PMID:40278683

In vitro effects of Mim8 and combined Mim8-bypassing therapy on thrombin generation, thromboelastography and fibrin clot ultrastructure., PMID:40398047

Babesia bigemina enolase binds to plasminogen and contains conserved B-cell epitopes that induce neutralizing antibodies in cattle., PMID:40403476

Mechanism and biological significance of erythrocyte homoaggregation (rouleaux formation): temperature-dependent entropic liberation of water from cells and macromolecules., PMID:40441594

Enfortumab vedotin induced interstitial lung disease: A first case report with pathological evidence from transbronchial lung cryobiopsy., PMID:40496844

IgA autoimmunity and coagulation among post-acute sequelae of SARS-CoV-2 infection (PASC) patients with persistent respiratory symptoms: a case-control study., PMID:40519899

Pharmacovigilance insights into antibody-drug conjugates: a multi-database analysis of adverse events in leukemia treatment., PMID:40522479

Endoplasmic reticulum protein 29 negatively regulates platelet functions and thrombosis in mice. [RHB99201]

Neutrophil extracellular traps block endogenous and intravenous thrombolysis-induced fibrinolysis in large vessel occlusion acute ischemic stroke. [RHB99201]

A novel role for thioredoxin-related transmembrane protein TMX4 in platelet activation and thrombus formation

Strategic reuse of rapid antigen tests for coagulation status assessment: an integrated machine learning approach. [RHB99201]

Datasheet
$ 372
Product specifications
100 μg 372 1 mg 1860

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For research use only. Not for human or drug use.

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Anti-Human Fibrin Antibody (59D8) [RHB99201]
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