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Anti-UBD Polyclonal Antibody

Catalog #:   PHA44901 Specific References (25) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: O15205
Overview

Catalog No.

PHA44901

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human UBD (Met1-Gly165).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Diubiquitin,UBD,Ubiquitin-like protein FAT10,Ubiquitin D,FAT10

Purification

Purified by antigen affinity column.

Accession

O15205

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with UBD antibody (PHA44901) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 21 kDa
    Observed MW: 21 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with UBD antibody (PHA44901) at 1 μg/ml.

    Lane 1: Hela
    Lane 2: SKOV
    Lane 3: MCF7
    Lane 4: A549
    Lane 5: SMMC

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 18 kDa
    Observed MW: 18 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse pancreas stained for UBD with PHA44901.
References

A novel and ultrasensitive electrochemical immunosensor based on nanocellulose-Ti3C2Tx@ZrO2 nano framework for the detection of ovalbumin., PMID:38092119

Nanohybrid nanozyme based colourimetric immunosensor for porcine gelatin., PMID:37979269

Heterologous prime-boost with mRNA-1273 stimulates persistent neutralising antibodies in BBIBP-CorV-vaccinated individuals., PMID:37880070

The ubiquitin-like protein FAT10 in hepatocellular carcinoma cells limits the efficacy of anti-VEGF therapy., PMID:37328057

Immunogenicity of COVID-19 vaccines and levels of SARS-CoV-2 neutralising antibody in the Bruneian population: Protocol for a national longitudinal study., PMID:36456015

Fluorescein-labeled ThUBD probe for super-sensitive visualization of polyubiquitination signal in situ cells., PMID:35710467

Electrochemiluminescence immunosensor for tropomyosin using carbon nanohorns/Nafion/Fe3O4@Pd screen-printed electrodes., PMID:32683494

Treatment of COVID-19: old tricks for new challenges., PMID:32178711

Investigation of patients with unclassified bleeding disorder and abnormal thrombin generation for physiological coagulation inhibitors reveals multiple abnormalities and a subset of patients with increased tissue factor pathway inhibitor activity., PMID:32003946

An ultra-sensitive label-free electrochemiluminescence CKMB immunosensor using a novel nanocomposite-modified printed electrode., PMID:35529968

Ubiquitin D is Upregulated by Synergy of Notch Signalling and TNF-α in the Inflamed Intestinal Epithelia of IBD Patients., PMID:30395194

Celiac disease biomarkers identified by transcriptome analysis of small intestinal biopsies., PMID:30097691

AuNPs/CNOs/SWCNTs/chitosan-nanocomposite modified electrochemical sensor for the label-free detection of carcinoembryonic antigen., PMID:29471282

A label free electrochemical immunosensor for sensitive detection of porcine serum albumin as a marker for pork adulteration in raw meat., PMID:27041316

Enhanced Purification of Ubiquitinated Proteins by Engineered Tandem Hybrid Ubiquitin-binding Domains (ThUBDs)., PMID:27037361

A carbon nanofiber-based label free immunosensor for high sensitive detection of recombinant bovine somatotropin., PMID:25794957

DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage., PMID:23042607

Changes in salivary immunoglobulin A (IgA) following match-play and training among English premiership footballers., PMID:22822634

The proteomic analysis of endogenous FAT10 substrates identifies p62/SQSTM1 as a substrate of FAT10ylation., PMID:22797925

Terminal galactosylation of glycoconjugates in Plasmodium falciparum asexual blood stages and Trypanosoma brucei bloodstream trypomastigotes., PMID:22406352

Detection and analysis of FAT10 modification., PMID:22350880

The role of innate immunity in the pathogenesis of preneoplasia in drug-induced chronic hepatitis based on a mouse model., PMID:21820428

FAT10 modifies p53 and upregulates its transcriptional activity., PMID:21396347

Expression of the FAT10 gene is highly upregulated in hepatocellular carcinoma and other gastrointestinal and gynecological cancers., PMID:12730673

The ubiquitin-like protein FAT10 forms covalent conjugates and induces apoptosis., PMID:11445583

Datasheet

Document Download

Anti-UBD Polyclonal Antibody.pdf

 

$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-UBD Polyclonal Antibody [PHA44901]
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