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Recombinant CSFV NS5A Protein, N-His

Catalog #:   YVV10402 Specific References (130) DATASHEET
Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Expression system: E. coli
Accession: AAT85717.1
Protein length: Ser2684-Leu3180
Overview

Catalog No.

YVV10402

Expression system

E. coli

Species

Classical swine fever virus (CSFV)

Protein length

Ser2684-Leu3180

Predicted molecular weight

58.31 kDa

Nature

Recombinant

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Accession

AAT85717.1

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

Genome polyprotein, polyprotein, NS5A, Non-structural protein 5A

Data Image
  • SDS-PAGE
    SDS PAGE for recombinant African swine fever virus Genome polyprotein
References

Evolution and antiviral activity of a human protein of retroviral origin. PMID: 36302021

Overview of hepatitis C virus genome structure, polyprotein processing, and protein properties. PMID: 10592656

Origins and evolutionary consequences of ancient endogenous retroviruses. PMID: 30962577

Insights into Polyprotein Processing and RNA-Protein Interactions in Foot-and-Mouth Disease Virus Genome Replication. PMID: 37154761

Polyprotein-Driven Formation of Two Interdependent Sets of Complexes Supporting Hepatitis C Virus Genome Replication. PMID: 26719260

[Basic information of Coronavirus]. PMID: 33967109

Selective Polyprotein Processing Determines Norovirus Sensitivity to Trim7. PMID: 35972292

Complete genome sequence of aucuba ringspot virus. PMID: 33554288

Viral precursor polyproteins: keys of regulation from replication to maturation. PMID: 23602469

Intercellular Communication in the Nervous System Goes Viral. PMID: 33485691

Genome sequence of pineapple secovirus B, a second sadwavirus reported infecting Ananas comosus. PMID: 36269415

Complete nucleotide sequence of polyprotein gene 1 and genome organization of turkey coronavirus. PMID: 18514348

Sapovirus-like particles derived from polyprotein. PMID: 18674574

Structural determinants and mechanism of HIV-1 genome packaging. PMID: 21762803

Complete genome sequence of a novel potyvirus infecting Miscanthus sinensis (silver grass). PMID: 35579714

Complete genome sequence of iris potyvirus B infecting Lilium lancifolium in China. PMID: 35925395

Comparative analysis of the genome structure and organization of the Middle East respiratory syndrome coronavirus (MERS-CoV) 2012 to 2019 revealing evidence for virus strain barcoding, zoonotic transmission, and selection pressure. PMID: 32803835

Expanding Repertoire of Plant Positive-Strand RNA Virus Proteases. PMID: 30650571

A comparative analysis of parechovirus protein structures with other picornaviruses. PMID: 34315275

HIV Gag polyprotein: processing and early viral particle assembly. PMID: 23266279

Complete genome sequence of ornithogalum virus 3. PMID: 33502594

The nematode polyprotein allergens/antigens. PMID: 10951596

Forcing the ribosome to change its message. PMID: 32414911

Complete genome sequence and genome characterization of a novel potyvirus from Lamprocapnos spectabilis. PMID: 36593436

Complete genome sequence and genome organization of scorzonera virus A (SCoVA), a novel member of the genus Potyvirus. PMID: 34363533

Characterization of a Cytopathogenic Reporter CSFV. PMID: 34201706

On the concept and elucidation of endogenous retroviruses. PMID: 23938748

Complete genome sequence of Amazon lily mosaic virus isolated from amaryllis (Hippeastrum hybridum Hort.). PMID: 35482088

Rabbit hemorrhagic disease virus: genome organization and polyprotein processing of a calicivirus studied after transient expression of cDNA constructs. PMID: 11040126

On the Selective Packaging of Genomic RNA by HIV-1. PMID: 27626441

Ty3, a Position-specific Retrotransposon in Budding Yeast. PMID: 26104707

Molecular virology of the hepatitis C virus. PMID: 10622560

Complete genome sequence of a recombinant isolate of yambean mosaic virus from Canavalia ensiformis. PMID: 34415501

Pseudo-polyprotein translated from the full-length ORF1 of capillovirus is important for pathogenicity, but a truncated ORF1 protein without variable and CP regions is sufficient for replication. PMID: 20381551

The Biological Impact of the Hypervariable N-Terminal Region of Potyviral Genomes. PMID: 31299166

Properties and functions of the nucleocapsid protein in virus assembly. PMID: 21157181

A review on HCV inhibitors: Significance of non-structural polyproteins. PMID: 30639895

Translation of the F protein of hepatitis C virus is initiated at a non-AUG codon in a +1 reading frame relative to the polyprotein. PMID: 15755749

Complete genome sequence of a novel human parechovirus. PMID: 23043176

Analysis of the RdRp, intergenic and structural polyprotein regions, and the complete genome sequence of Kashmir bee virus from infected honeybees (Apis mellifera) in Korea. PMID: 24824301

Genomic characterization of a novel potyvirus infecting Barleria repens in South Africa. PMID: 36576587

Retroviral Gag protein-RNA interactions: Implications for specific genomic RNA packaging and virion assembly. PMID: 29580971

Crystal structure of a novel conformational state of the flavivirus NS3 protein: implications for polyprotein processing and viral replication. PMID: 19793813

Foamy viruses are unconventional retroviruses. PMID: 9971751

Temporal Proteomic and Phosphoproteomic Analysis of EV-A71-Infected Human Cells. PMID: 36106392

The piRNA Response to Retroviral Invasion of the Koala Genome. PMID: 31607510

Genetic characteristics of the complete feline kobuvirus genome. PMID: 25404141

Beyond plasma membrane targeting: role of the MA domain of Gag in retroviral genome encapsidation. PMID: 21762800

The human foamy virus pol gene is expressed as a Pro-Pol polyprotein and not as a Gag-Pol fusion protein. PMID: 8551561

Complete genome sequence of zoysia mosaic virus, a novel member of the genus Poacevirus. PMID: 37043050

[Molecular virology of hepatitis C]. PMID: 12564041

Leader protein of encephalomyocarditis virus binds zinc, is phosphorylated during viral infection, and affects the efficiency of genome translation. PMID: 11883190

Specific binding of human immunodeficiency virus type 1 gag polyprotein and nucleocapsid protein to viral RNAs detected by RNA mobility shift assays. PMID: 8230441

Expanding our understanding of the role polyprotein conformation plays in the coronavirus life cycle. PMID: 32348474

Complete genome sequences of two insect-specific flaviviruses. PMID: 28918473

The RNA-dependent RNA polymerase of the infectious pancreatic necrosis virus is linked to viral mRNA acting as a cap substitute. PMID: 35349401

Visualization of Retroviral Gag-Genomic RNA Cellular Interactions Leading to Genome Encapsidation and Viral Assembly: An Overview. PMID: 35215917

Molecular characterization of a novel endornavirus isolated from Ophiostoma bicolor associated with bark beetles. PMID: 36227426

Iranian johnsongrass mosaic virus: the complete genome sequence, molecular and biological characterization, and comparison of coat protein gene sequences. PMID: 27632283

Characterization of a New Nepovirus Infecting Grapevine. PMID: 33048594

Analysis of the nonstructural and structural polyprotein regions, and complete genome sequences of Israel acute paralysis viruses identified from honeybees (Apis mellifera) in Korea. PMID: 23886494

Sensitivity of Alphaviruses to G3BP Deletion Correlates with Efficiency of Replicase Polyprotein Processing. PMID: 31941782

Paleovirology of 'syncytins', retroviral env genes exapted for a role in placentation. PMID: 23938756

Complete genome sequence of passiflora virus Y infecting passion fruit in China. PMID: 33635433

Modulation of proteolytic polyprotein processing by coxsackievirus mutants resistant to inhibitors targeting phosphatidylinositol-4-kinase IIIβ or oxysterol binding protein. PMID: 29024767

The nonmyristylated Pr160gag-pol polyprotein of human immunodeficiency virus type 1 interacts with Pr55gag and is incorporated into viruslike particles. PMID: 1383561

A new flavi-like virus identified in populations of wild carrots. PMID: 35962825

Updated SARS-CoV-2 single nucleotide variants and mortality association. PMID: 34245452

Robust clinical detection of SARS-CoV-2 variants by RT-PCR/MALDI-TOF multitarget approach. PMID: 34877674

Genome Analysis of a Novel Tembusu Virus in Taiwan. PMID: 32455871

Lack of evidence for ribosomal frameshifting in ATP7B mRNA decoding. PMID: 36115342

Adventure of the CRISPR Coronavirus Disease 2019 Diagnostic. PMID: 33560912

Proteolytic processing of sapovirus ORF1 polyprotein. PMID: 15919882

Presence of complete murine viral genome sequences in patient-derived xenografts. PMID: 33795676

HIV nucleocapsid protein. Expression in Escherichia coli, purification, and characterization. PMID: 8344933

Genome sequence and geographic distribution of a new nepovirus infecting Stenotaphrum secundatum in Australia. PMID: 34487768

Analysis of the complete genome sequence of euphorbia ringspot virus, an atypical member of the genus Potyvirus. PMID: 27686070

Processing map and essential cleavage sites of the nonstructural polyprotein encoded by ORF1 of the feline calicivirus genome. PMID: 12072506

Strawberry Mottle Virus (Family Secoviridae, Order Picornavirales) Encodes a Novel Glutamic Protease To Process the RNA2 Polyprotein at Two Cleavage Sites. PMID: 30541838

Role of HIV-1 Gag domains in viral assembly. PMID: 12873766

Modifications to the Foot-and-Mouth Disease Virus 2A Peptide: Influence on Polyprotein Processing and Virus Replication. PMID: 29386286

Genomic and epidemiological characteristics of SARS-CoV-2 in Africa. PMID: 33901167

Diverse mosquito-specific flaviviruses in the Bolivian Amazon basin. PMID: 33416463

Plant expression of NifD protein variants resistant to mitochondrial degradation. PMID: 32868448

Analysis of public domain plant transcriptomes expands the phylogenetic diversity of the family Secoviridae. PMID: 36040568

Variant-Specific Analysis Reveals a Novel Long-Range RNA-RNA Interaction in SARS-CoV-2 Orf1a. PMID: 36232353

Effect of the CSFV NS5A protein on key proteins in the MAPK and PI3K-mTOR signaling pathways in porcine macrophages., PMID:40078537

PKM2 induces mitophagy through the AMPK-mTOR pathway promoting CSFV proliferation., PMID:38319105

Evolutionary-Related High- and Low-Virulent Classical Swine Fever Virus Isolates Reveal Viral Determinants of Virulence., PMID:38275957

Classical swine fever virus NS5A protein activates autophagy via the PP2A-DAPK3-Beclin 1 axis., PMID:38038430

Classical swine fever virus NS5A protein antagonizes innate immune response by inhibiting the NF-κB signaling., PMID:37714433

The Small GTPase Rab14 Regulates the Trafficking of Ceramide from Endoplasmic Reticulum to Golgi Apparatus and Facilitates Classical Swine Fever Virus Assembly., PMID:37255314

Intracellular Vimentin Regulates the Formation of Classical Swine Fever Virus Replication Complex through Interaction with NS5A Protein., PMID:37129496

HSP90AA1 interacts with CSFV NS5A protein and regulates CSFV replication via the JAK/STAT and NF-κB signaling pathway., PMID:36405689

Mitophagy induced by classical swine fever virus nonstructural protein 5A promotes viral replication., PMID:35948130

Autophagy Induced by the N-Terminus of the Classic Swine Fever Virus Nonstructural Protein 5A Protein Promotes Viral Replication., PMID:34512612

ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells., PMID:33721634

Porcine RACK1 negatively regulates the infection of classical swine fever virus and the NF-κB activation in PK-15 cells., PMID:32605753

Rab18 binds to classical swine fever virus NS5A and mediates viral replication and assembly in swine umbilical vein endothelial cells., PMID:32419589

Induction of autophagy and suppression of type I IFN secretion by CSFV., PMID:32160078

CSFV protein NS5A activates the unfolded protein response to promote viral replication., PMID:32056717

Viperin inhibits classical swine fever virus replication by interacting with viral nonstructural 5A protein., PMID:31517388

Classical swine fever virus non-structural proteins modulate Toll-like receptor signaling pathways in porcine monocyte-derived macrophages., PMID:30827374

Cellular Hsp27 interacts with classical swine fever virus NS5A protein and negatively regulates viral replication by the NF-κB signaling pathway., PMID:29525670

The eukaryotic translation initiation factor 3 subunit E binds to classical swine fever virus NS5A and facilitates viral replication., PMID:29223786

Rab1A is required for assembly of classical swine fever virus particle., PMID:29128753

Rab5 Enhances Classical Swine Fever Virus Proliferation and Interacts with Viral NS4B Protein to Facilitate Formation of NS4B Related Complex., PMID:28848503

The effect of classical swine fever virus NS5A and NS5A mutants on oxidative stress and inflammatory response in swine testicular cells., PMID:28142057

Identification of cleavage of NS5A of C-strain classical swine fever virus., PMID:27766426

Classical swine fever virus NS5A protein changed inflammatory cytokine secretion in porcine alveolar macrophages by inhibiting the NF-κB signaling pathway., PMID:27296632

In vitro adaptation and genome analysis of a sub-subgenotype 2.1c isolate of classical swine fever virus., PMID:27155669

Guanylate-Binding Protein 1, an Interferon-Induced GTPase, Exerts an Antiviral Activity against Classical Swine Fever Virus Depending on Its GTPase Activity., PMID:26889038

FKBP8 interact with classical swine fever virus NS5A protein and promote virus RNA replication., PMID:26748656

eEF1A Interacts with the NS5A Protein and Inhibits the Growth of Classical Swine Fever Virus., PMID:26266418

Heat shock protein 70 is associated with CSFV NS5A protein and enhances viral RNA replication., PMID:25827528

Annexin A2 is involved in the production of classical swine fever virus infectious particles., PMID:25593157

Characterization of the C-terminal sequence of NS5A necessary for the assembly and production of classical swine fever virus infectious particles., PMID:25218811

Screening of cellular proteins that interact with the classical swine fever virus non-structural protein 5A by yeast two-hybrid analysis., PMID:24499791

Proteome-wide screening reveals immunodominance in the CD8 T cell response against classical swine fever virus with antigen-specificity dependent on MHC class I haplotype expression., PMID:24376799

Autophagy enhances the replication of classical swine fever virus in vitro., PMID:24262968

Classical swine fever virus NS5A regulates viral RNA replication through binding to NS5B and 3'UTR., PMID:22795973

Classical swine fever virus NS5A protein localizes to endoplasmic reticulum and induces oxidative stress in vascular endothelial cells., PMID:22718084

Classical swine fever virus NS5A protein interacts with 3'-untranslated region and regulates viral RNA synthesis., PMID:22261205

Classical swine fever virus NS5B protein suppresses the inhibitory effect of NS5A on viral translation by binding to NS5A., PMID:22258858

Biosynthesis of classical swine fever virus nonstructural proteins., PMID:21270154

Development of a highly sensitive real-time RT-PCR protocol for the detection of Classical swine fever virus independent of the 5' untranslated region., PMID:21111760

Classical swine fever virus NS3 is an IRES-binding protein and increases IRES-dependent translation., PMID:20637813

Characterization of NS3, NS5A and NS5B of classical swine fever virus through mutation and complementation analysis., PMID:19713054

Influence of NS5A protein of classical swine fever virus (CSFV) on CSFV internal ribosome entry site-dependent translation., PMID:19710255

Complete genomic sequence of border disease virus, a pestivirus from sheep., PMID:9573288

Datasheet
$ 313
Product specifications
100 μg 313 1 mg 1629

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Recombinant CSFV NS5A Protein, N-His [YVV10402]
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