Catalog No.
DHF39902
Description
MEDI3185 has picomolar binding affinity to human CXCR4 and exhibits no significant binding to other chemokine receptors such as CCR4 or CXCR3. In vitro studies demonstrated that MEDI3185 inhibited tumor cell migration, blocked SDF-1 induced tumor cell signaling and induced apoptosis of tumor cells. The MEDI3185 epitope comprises the entire ECL2 sequence, lacks any so-called 'hot-spot' and is remarkably resistant to mutations. The structure of MEDI3185 variable domains was modeled, and suggested a β-strand/β-strand interaction between MEDI3185 CDR3H and CXCR4 ECL2, resulting in direct steric hindrance with CXCR4 ligand SDF-1.
Expression system
Mammalian Cells
Species reactivity
Human
Host species
Human
Isotype
IgG1
Clonality
Monoclonal
Target
Stromal cell-derived factor 1 receptor, LCR1, HM89, LPS-associated protein 3, LAP-3, CXC-R4, CXCR-4, SDF-1 receptor, CD184, NPYRL, LESTR, Fusin, FB22, C-X-C chemokine receptor type 4, CXCR4, Lipopolysaccharide-associated protein 3, Leukocyte-derived seven transmembrane domain receptor
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P61073
Applications
Research Grade Biosimilar
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
MEDI3185
Molecular basis for the antagonistic activity of an anti-CXCR4 antibody. PMID: 26514996
Evaluation of assay interference and interpretation of CXCR4 receptor occupancy results in a preclinical study with MEDI3185, a fully human antibody to CXCR4. PMID: 26384735
Functional mimetic of the G-protein coupled receptor CXCR4 on a soluble antibody scaffold. PMID: 30900513
Immune suppressive activities of low-density neutrophils in sepsis and potential use as a novel biomarker of sepsis-induced immune suppression., PMID:40108283
Investigating the effect of immunomagnetic separation on the immunophenotype and viability of plasma cells in plasma cell disorders., PMID:39493694
Cx4Mab-1: A Novel Anti-Mouse CXCR4 Monoclonal Antibody for Flow Cytometry., PMID:38126879
Impact of Hemin on Interleukin-21 Levels and Plasma Cells in Transfusion-Dependent Thalassemia with Positive and Negative Allo-Autoantibody., PMID:36636711
Production of human embryonic kidney 293T cells stably expressing C-X-C chemokine receptor type 4 (CXCR4) as a screening tool for anticancer lead compound targeting CXCR4., PMID:35643380
Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia., PMID:34136120
Delineation of target expression profiles in CD34+/CD38- and CD34+/CD38+ stem and progenitor cells in AML and CML., PMID:33085758
Differential Expression of IgM and IgD Discriminates Two Subpopulations of Human Circulating IgM+IgD+CD27+ B Cells That Differ Phenotypically, Functionally, and Genetically., PMID:32435242
Exhaustion of the bone marrow progenitor cell reserve is associated with major events in severe limb ischemia., PMID:30929097
Effect of Different Regimens of Co-Transplantation of Hemopoietic and Mesenchymal Hemopoietic Stem Cells on the Rates of Hemopoietic Recovery in Laboratory Mice Treated with Cyclophosphamide in Sublethal Doses., PMID:30783845
Increased extracellular ubiquitin in surgical wound fluid provides a chemotactic signal for myeloid dendritic cells., PMID:30159662
Phenotyping and Target Expression Profiling of CD34+/CD38- and CD34+/CD38+ Stem- and Progenitor cells in Acute Lymphoblastic Leukemia., PMID:29772458
Mimicking the tumour microenvironment of chronic lymphocytic leukaemia in vitro critically depends on the type of B-cell receptor stimulation., PMID:26924423
Multiple myeloma: New surface antigens for the characterization of plasma cells in the era of novel agents., PMID:26287276
Characterization of B and plasma cells in blood, bone marrow, and secondary lymphoid organs of rhesus macaques by multicolor flow cytometry., PMID:25341725
Regulatory T cells, interleukin (IL)-6, IL-8, vascular endothelial growth factor (VEGF), CXCL10, CXCL11, epidermal growth factor (EGF) and hepatocyte growth factor (HGF) as surrogate markers of host immunity in patients with renal cell carcinoma., PMID:23495770
Circulating hematopoietic stem cell count is a valuable predictor of prematurity complications in preterm newborns., PMID:22985188
Maintenance and characterization of multipotent mesenchymal stem cells isolated from canine umbilical cord matrix by collagenase digestion., PMID:22975229
Survival and differentiation of human embryonic stem cell-derived neural precursors grafted spinally in spinal ischemia-injured rats or in naive immunosuppressed minipigs: a qualitative and quantitative study., PMID:22889456
α1Proteinase inhibitor regulates CD4+ lymphocyte levels and is rate limiting in HIV-1 disease., PMID:22363634
Immunophenotyping of classic murine myeloma cell lines used for monoclonal antibody production., PMID:22316479
AMD3100 disrupts the cross-talk between chronic lymphocytic leukemia cells and a mesenchymal stromal or nurse-like cell-based microenvironment: pre-clinical evidence for its association with chronic lymphocytic leukemia treatments., PMID:22058221
Cell-surface marker signatures for the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells., PMID:21407814
New monoclonal antibodies against B-cell antigens: possible new strategies for diagnosis of primary cutaneous B-cell lymphomas., PMID:20951741
Analysis of HLDA9 mAbs on plasmacytoid dendritic cells., PMID:20933012
Phenotyping studies of clonotypic B lymphocytes from patients with multiple myeloma by flow cytometry., PMID:19792049
A critical appraisal of factors affecting the accuracy of results obtained when using flow cytometry in stem cell investigations: where do you put your gates?, PMID:19562683
Quantification and immunophenotypic characterization of bone marrow and umbilical cord blood mesenchymal stem cells by multicolor flow cytometry., PMID:19376394
Bortezomib overcomes cell-adhesion-mediated drug resistance through downregulation of VLA-4 expression in multiple myeloma., PMID:18850009
CXCR4 pos circulating progenitor cells coexpressing monocytic and endothelial markers correlating with fibrotic clinical features are present in the peripheral blood of patients affected by systemic sclerosis., PMID:18556411
Extensive flow cytometric characterization of plasmacytoid dendritic cell leukemia cells., PMID:16146542
Functional expression of CXCR4 (CD184) on small-cell lung cancer cells mediates migration, integrin activation, and adhesion to stromal cells., PMID:14603250
CXCR4 chemokine receptors (CD184) and alpha4beta1 integrins mediate spontaneous migration of human CD34+ progenitors and acute myeloid leukaemia cells beneath marrow stromal cells (pseudoemperipolesis)., PMID:12899713
Detection of novel CD antigens on the surface of human mast cells and basophils., PMID:12021549
CD30 triggering by agonistic antibodies regulates CXCR4 expression and CXCL12 chemotactic activity in the cell line L540., PMID:11756152
For research use only. Not for human or drug use.
