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Anti-Human CD81 Antibody (005-C01)

Catalog #:   RHF34001 Specific References (50) DATASHEET
Host species: Human
Isotype: IgG1
Applications: ELISA, FCM, IHC, WB
Expression system: Mammalian Cells
Overview

Catalog No.

RHF34001

Description

CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells. In muscles, CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.

Expression system

Mammalian Cells

Species reactivity

Human

Host species

Human

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:200, WB: 1:500-1:4000

Target

CD81 antigen,TSPAN28,Target of the antiproliferative antibody 1,Tspan-28,Tetraspanin-28,CD81,TAPA1,26 kDa cell surface protein TAPA-1

Concentration

1.03 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P60033

Applications

ELISA, FCM, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

005-C01

Data Image
  • Bioactivity
    Detects CD81 in indirect ELISAs.
  • Flow-cytometry
    Flow-cytometry using anti-human CD81 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD81 antibody monoclonal antibody (Catalog # RHF34001 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • SEC-HPLC
    The purity of this product is >95% as determined by SEC-HPLC.
References

Glycoengineering of the hepatitis C virus E2 glycoprotein improves biochemical properties and enhances immunogenicity., PMID:40500295

Ultrasound-triggered targeted delivery of engineered ADSCs-derived exosomes with high SDF-1α levels to promote cardiac repair following myocardial infarction., PMID:40460966

An Extracellular Vesicle (EV) Paper Strip for Rapid and Convenient Estimation of EV Concentration., PMID:40422033

Development of a high-sensitivity assay for direct quantitation of EGFr on extracellular vesicles in plasma., PMID:40345522

[Efficient capture and proteomics analysis of urinary extracellular vesicles by affinity purification]., PMID:40331614

Extracellular Vesicle Marker Changes Associated With Disease Activity in Relapsing-Remitting Multiple Sclerosis., PMID:40300121

Glycoengineering of the hepatitis C virus E2 glycoprotein leads to improved biochemical properties and enhanced immunogenicity., PMID:40291659

Microfluidic Nano-Plasmonic Imaging Platform for Purification- and Label-Free Single Small Extracellular Vesicle Counting., PMID:40166220

Nanoscopic Profiling of Small Extracellular Vesicles via High-Speed Atomic Force Microscopy (HS-AFM) Videography., PMID:40139685

Next Generation Aqueous Two-Phase System for Gentle, Effective, and Timely Extracellular Vesicle Isolation and Transcriptomic Analysis., PMID:40108918

Isolation of Urinary Extracellular Vesicles (EVs) via Hydrophobic Interaction Chromatography Using a Nylon-6 Capillary-Channeled Polymer (C-CP) Fiber Column., PMID:39933961

EV-Lev: extracellular vesicle isolation from human plasma using microfluidic magnetic levitation device., PMID:39918033

Tetraspanin CD81 serves as a functional entry factor for porcine circovirus type 2 infection., PMID:39745447

EV-Elute: A universal platform for the enrichment of functional surface marker-defined extracellular vesicle subpopulations., PMID:39692115

A novel multiple marker microarray analyzer and methodology to predict major obstetric syndromes using surface markers of circulating extracellular vesicles from maternal plasma., PMID:39607297

Streamlined miRNA loading of surface protein-specific extracellular vesicle subpopulations through electroporation., PMID:39574085

Multi-omic characteristics of longitudinal immune profiling after breakthrough infections caused by Omicron BA.5 sublineages., PMID:39536392

Investigating the effect of immunomagnetic separation on the immunophenotype and viability of plasma cells in plasma cell disorders., PMID:39493694

Citrullination Accompanies the Development of Carotid Atherosclerotic Plaques., PMID:39421992

Comparison of nanoimaging and nanoflow based detection of extracellular vesicles at a single particle resolution., PMID:39416671

Enhancing Extracellular Vesicle Detection via Cotargeting Tetraspanin Biomarkers., PMID:39360503

Multiparametric profiling of HER2-enriched extracellular vesicles in breast cancer using Single Extracellular VEsicle Nanoscopy., PMID:39342336

Sensitive phenotyping of serum extracellular vesicles on a SERS-microfluidic platform for early-stage clinical diagnosis of ovarian carcinoma., PMID:39260102

Pharmacologic HIF stabilization activates costimulatory receptor expression to increase antitumor efficacy of adoptive T cell therapy., PMID:39196939

Extracellular vesicles derived from antigen-presenting cells pulsed with foot and mouth virus vaccine-antigens act as carriers of viral proteins and stimulate B cell response., PMID:39176090

Hepatitis C virus chronicity and oncogenic potential: Vaccine development progress., PMID:39167987

CD81 fusion alters SARS-CoV-2 Spike trafficking., PMID:39140770

Comparison of EV characterization by commercial high-sensitivity flow cytometers and a custom single-molecule flow cytometer., PMID:39140467

Urinary Extracellular Vesicles as a Readily Available Biomarker Source: A Simplified Stratification Method., PMID:39125575

Exosomal membrane proteins analysis using a silicon nanowire field effect transistor biosensor., PMID:39002259

Immunoassays for Extracellular Vesicle Detection via Transmembrane Proteins Using Surface Plasmon Resonance Biosensors., PMID:38912608

Circulating small extracellular vesicles in Alzheimer's disease: a case-control study of neuro-inflammation and synaptic dysfunction., PMID:38902659

Isolation and quantification of L1CAM-positive extracellular vesicles on a chip as a potential biomarker for Parkinson's Disease., PMID:38898558

Fine-tuning spatial-temporal dynamics and surface receptor expression support plasma cell-intrinsic longevity., PMID:38896451

Impact of brain organoid-derived sEVs on metastatic adaptation and invasion of breast carcinoma cells through a microphysiological system., PMID:38888211

Computational Study on the Wealth Conformations of Hepatitis C Virus Envelope Glycoprotein 2 and Role of Its Glycan Coat., PMID:38861660

Alterations in B and NK cells highly correlate with disease severity in children with COVID-19., PMID:38813014

Immune checkpoint inhibitors for POLE or POLD1 proofreading-deficient metastatic colorectal cancer., PMID:38777726

High-throughput proteomic analysis of extracellular vesicles from saliva by chemical probe-based array., PMID:38772652

The antitumor effect of extracellular vesicles derived from cytokine-activated CD8+ T cells., PMID:38753658

CD71: Role in permafrost immunity., PMID:38711995

Exhaled breath condensate contains extracellular vesicles (EVs) that carry miRNA cargos of lung tissue origin that can be selectively purified and analyzed., PMID:38659349

Spectroscopic insight into breast cancer: profiling small extracellular vesicles lipids via infrared spectroscopy for diagnostic precision., PMID:38654096

[Exosomes' role in intercellular interactions in different variants of lung injury in fatal cases of COVID-19]., PMID:38591903

A molecular docking exploration of the large extracellular loop of tetraspanin CD81 with small molecules., PMID:38584777

Utilizing Magnetic Levitation to Detect Lung Cancer-Associated Exosomes., PMID:38520356

Isoprinosine as a foot-and-mouth disease vaccine adjuvant elicits robust host defense against viral infection through immunomodulation., PMID:38510965

Faster clinical decisions in B-cell acute lymphoblastic leukaemia: A single flow cytometric 12-colour tube improves diagnosis and minimal residual disease follow-up., PMID:38432068

Decoding of the surfaceome and endocytome in primary glioblastoma cells identifies potential target antigens in the hypoxic tumor niche., PMID:38414005

Negative Charge-Carrying Glycans Attached to Exosomes as Novel Liquid Biopsy Marker., PMID:38400284

Datasheet
$ 372
Product specifications
100 μg 372 1 mg 1860

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human CD81 Antibody (005-C01) [RHF34001]
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