Catalog No.
YHG74201
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Gly173-Arg397
Predicted molecular weight
28.30 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q14184
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
DOC2B, Doc2-beta, Double C2-like domain-containing protein beta, DOC2BL
The ester derivative Palmitoylcarnitine abrogates cervical cancer cell survival by enhancing lipotoxicity and mitochondrial dysfunction., PMID:40319292
DOC2b enrichment mitigates proinflammatory cytokine-induced CXCL10 expression by attenuating IKKβ and STAT-1 signaling in human islets., PMID:39805534
DOC2b enrichment mitigates proinflammatory cytokine-induced CXCL10 expression by attenuating IKKβ and STAT-1 signaling in human islets., PMID:39763877
Biological functions of extracellular vesicle double C2-like domain beta in cervical cancer., PMID:39747389
Pancreatic β-cells package double C2-like domain beta protein into extracellular vesicles via tandem C2 domains., PMID:39497805
Comprehensive Analysis of Crucial m6A-Related Differentially Expressed Genes in Psoriasis., PMID:39344312
Integrated In-Silico and In Vitro analysis to Decipher the contribution of bisphenol-A in cervical cancer., PMID:38555994
Disease-linked mutations in Munc18-1 deplete synaptic Doc2., PMID:38242640
A Novel Role for DOC2B in Ameliorating Palmitate-Induced Glucose Uptake Dysfunction in Skeletal Muscle Cells via a Mechanism Involving β-AR Agonism and Cofilin., PMID:38203312
Double C-2 like domain beta (DOC2B) induces calcium dependent oxidative stress to promote lipotoxicity and mitochondrial dysfunction for its tumor suppressive function., PMID:36913987
Dopaminergic Neurons Differentiated from LRRK2 I1371V-Induced Pluripotent Stem Cells Display a Lower Yield, α-Synuclein Pathology, and Functional Impairment., PMID:36006382
DOC2B is a negative regulator of Wnt/β-catenin signaling pathway in cervical cancer., PMID:35500882
DOC2b Enhances β-Cell Function via a Novel Tyrosine Phosphorylation-Dependent Mechanism., PMID:35377441
Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence., PMID:33758996
Ca2+ sensor proteins in spontaneous release and synaptic plasticity: Limited contribution of Doc2c, rabphilin-3a and synaptotagmin 7 in hippocampal glutamatergic neurons., PMID:33753311
Conventional and Unconventional Mechanisms by which Exocytosis Proteins Oversee β-cell Function and Protection., PMID:33673206
Distinct subtypes of proprioceptive dorsal root ganglion neurons regulate adaptive proprioception in mice., PMID:33589589
Loss of Doc2b does not influence transmission at Purkinje cell to deep nuclei synapses under physiological conditions., PMID:32347796
Doc2 Proteins Are Not Required for the Increased Spontaneous Release Rate in Synaptotagmin-1-Deficient Neurons., PMID:32098902
Synaptotagmin-1 and Doc2b Exhibit Distinct Membrane-Remodeling Mechanisms., PMID:31952804
Doc2b Ca2+ binding site mutants enhance synaptic release at rest at the expense of sustained synaptic strength., PMID:31594980
Phosphatidylinositol 4,5-bisphosphate drives Ca2+-independent membrane penetration by the tandem C2 domain proteins synaptotagmin-1 and Doc2β., PMID:31147445
DOC2B promotes insulin sensitivity in mice via a novel KLC1-dependent mechanism in skeletal muscle., PMID:30707251
Protein kinase C iota facilitates insulin-induced glucose transport by phosphorylation of soluble nSF attachment protein receptor regulator (SNARE) double C2 domain protein b., PMID:30369065
Excitatory and Inhibitory Neurons Utilize Different Ca2+ Sensors and Sources to Regulate Spontaneous Release., PMID:29754754
Doc2b Protects β-Cells Against Inflammatory Damage and Enhances Function., PMID:29661782
Exocytosis Protein DOC2B as a Biomarker of Type 1 Diabetes., PMID:29506054
Doc2B acts as a calcium sensor for vesicle priming requiring synaptotagmin-1, Munc13-2 and SNAREs., PMID:29274147
Phosphatidylinositol (4, 5)-bisphosphate targets double C2 domain protein B to the plasma membrane., PMID:28941037
Update on GLUT4 Vesicle Traffic: A Cornerstone of Insulin Action., PMID:28602209
Exocytosis proteins as novel targets for diabetes prevention and/or remediation?, PMID:28356294
Regulation of Ca2+ channels by SNAP-25 via recruitment of syntaxin-1 from plasma membrane clusters., PMID:27605709
Ring-like oligomers of Synaptotagmins and related C2 domain proteins., PMID:27434670
Direct quantitative detection of Doc2b-induced hemifusion in optically trapped membranes., PMID:26395669
Tctex1d2 Is a Negative Regulator of GLUT4 Translocation and Glucose Uptake., PMID:26200093
Structural elements that underlie Doc2β function during asynchronous synaptic transmission., PMID:26195798
Doc2b serves as a scaffolding platform for concurrent binding of multiple Munc18 isoforms in pancreatic islet β-cells., PMID:25190515
DOC2 isoforms play dual roles in insulin secretion and insulin-stimulated glucose uptake., PMID:25005332
Doc2b enrichment enhances glucose homeostasis in mice via potentiation of insulin secretion and peripheral insulin sensitivity., PMID:24705606
DNA promoter methylation-dependent transcription of the double C2-like domain β (DOC2B) gene regulates tumor growth in human cervical cancer., PMID:24570007
Molecular characterization of near-complete trisomy 17p syndrome from inverted duplication in association with cryptic deletion of 17pter., PMID:24393711
Mutations that disrupt Ca²⁺-binding activity endow Doc2β with novel functional properties during synaptic transmission., PMID:24356452
Doc2b synchronizes secretion from chromaffin cells by stimulating fast and inhibiting sustained release., PMID:24133251
Munc13-1 Translocates to the Plasma Membrane in a Doc2B- and Calcium-Dependent Manner., PMID:24062723
The C2B domain is the primary Ca2+ sensor in DOC2B: a structural and functional analysis., PMID:23994332
DOC2B and Munc13-1 differentially regulate neuronal network activity., PMID:23537531
Doc2b promotes GLUT4 exocytosis by activating the SNARE-mediated fusion reaction in a calcium- and membrane bending-dependent manner., PMID:23427263
Doc2b is a key effector of insulin secretion and skeletal muscle insulin sensitivity., PMID:22698913
Doc2 is a Ca2+ sensor required for asynchronous neurotransmitter release., PMID:22036572
Doc2 supports spontaneous synaptic transmission by a Ca(2+)-independent mechanism., PMID:21521611
For research use only. Not for human or drug use.
