Catalog No.
KAD10801
Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative sandwich enzyme immunoassay technique. FMC63 scFv has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-FMC63 scFv will be captured by immobilized FMC63 scFv. After washing away any unbound substances, a biotin-labeled FMC63 scFv is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-FMC63 scFv bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of Anti-FMC63 scFv concentration Human/Mouse serum, plasma and cell culture supernates.
Detection method
Colorimetric
Sample type
Plasma, Serum, cell culture supernates
Assay type
Quantitative
Range
0.94 - 60 ng/mL
Sensitivity
0.63 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
26.6 |
6.7 |
1.4 |
26.2 |
6.6 |
1.4 |
|
Standard deviation |
2.2 |
0.3 |
0.1 |
2.5 |
0.3 |
0.1 |
|
CV (%) |
8.1 |
3.9 |
8.6 |
9.7 |
4.3 |
9.2 |
Recovery
80-120%
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Alternative Names
Anti-FMC63 scFv antibody, CD19, CAR-T, Mouse FMC63 scFv
Background
FMC63 is a mouse IgG2a monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. Anti-FMC63 scFv antibody can specifically bind to the antigen recognition epitope of FMC63 scFv on anti-CD19 CAR, and it shows high specificity and sensitivity, it's used to detect the expression of FMC63 scFv derived CAR.
Note
For Research Use Only.
For research use only. Not for human or drug use.
