Catalog No.
RGK08503
Species reactivity
General
Host species
Mouse
Isotype
IgG2b
Clonality
Monoclonal
Tested applications
IF: 1:100-1:200, IP: 1:20, WB: 1:500-1:1000
Target
Flag tag, Flag tag (DYKDDDDK-tag), Flag-tag, Flag peptide (DYKDDDDK)
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Applications
IF, IP, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3Y40
Unraveling Botulinum Neurotoxin A Light-Chain-Induced Signaling Pathways: A Phosphoproteomic Analysis in a Controlled Cellular Model., PMID:40507977
Generation of Hoxa11-3XFLAG and Hoxd11-3XFLAG alleles to investigate Hox11 genome-wide binding., PMID:40389054
Identification of autoantibodies targeting citrullinated CLEC12A in rheumatoid arthritis patients., PMID:40336986
Multimerized epitope tags for high-sensitivity protein detection., PMID:40191928
Hypoxia-Inducible Factor Prolyl Hydroxylase Inhibitor, FG4592, Induces Endogenous Metallothionein3 Expression in Human Neuronal Cell Line, ReNcell CX Cells., PMID:39993744
Rescuing Newcastle disease virus with tag for screening viral-host interacting proteins based on highly efficient reverse genetics., PMID:39100766
Tracing the invisible mutant ADNP protein in Helsmoortel-Van der Aa syndrome patients., PMID:38926592
Structural Basis for Recognition of the FLAG-tag by Anti-FLAG M2., PMID:38852931
Flow Cytometry-Based Measurement of Antibodies Specific for Cell Surface-Expressed Folded SARS-CoV-2 Receptor-Binding Domains., PMID:38675759
Synthetic protein protease sensor platform., PMID:38646011
Characterization of ATG8-family protein phosphorylation by Phos-tag gel for autophagy study., PMID:38319785
Multiplex Assays for Analysis of Antibody Responses to South Asian Plasmodium falciparum and Plasmodium vivax Malaria Infections., PMID:38276660
[High-level expression of anti FLAG tag antibody in plants]., PMID:38258646
Pharmacokinetics of monoclonal antibodies locally-applied into the middle ear of guinea pigs., PMID:38218017
Insertion of a FLAG-tag sequence at the end of exon 9 of the TBX5 gene in three induced pluripotent stem cell lines (DHMi004-A-4, DHMi004-A-5, DHMi004-A-6) by CRISPR/Cas9 technology., PMID:38100916
Comparing in vitro affinity measurements of antibodies to TfR1: Surface plasmon resonance versus on-cell affinity., PMID:38006952
Recombinant rotavirus expressing the glycosylated S1 protein of SARS-CoV-2., PMID:37830788
Detection of Math6-Expressing Cell Types in Murine Placenta., PMID:37759651
Protocol to capture transcription factor-mediated 3D chromatin interactions using affinity tag-based BL-HiChIP., PMID:37738118
A quantitative comparison of antibodies against epitope tags for immunofluorescence detection., PMID:37702273
Production of Plant-Derived Japanese Encephalitis Virus Multi-Epitope Peptide in Nicotiana benthamiana and Immunological Response in Mice., PMID:37511402
Improving Single-Molecule Antibody Detection Selectivity through Optimization of Peptide Epitope Presentation in OmpG Nanopore., PMID:37379512
Production of recombinant His-tagged triple-FLAG peptide in Brevibacillus choshinensis and its utilization as an easy-to-remove affinity peptide., PMID:37328425
RNA and Protein Interactomes of an RNA-Binding Protein Tagged with FLAG Epitopes Using Combinatory Approaches of Genome Engineering and Stable Transfection., PMID:37166670
A novel epitope tag from rabies virus has versatile in vitro applications., PMID:37166480
Development of a 5-HT7 receptor antibody for the rat: the good, the bad, and the ugly., PMID:37071157
Epitope-tag-mediated synaptogenic activity in an engineered neurexin-1β lacking the binding interface with neuroligin-1., PMID:37030069
Core-Shell Magnetic Imprinted Polymers for the Recognition of FLAG-Tagpeptide., PMID:36834864
Insertion of exogenous genes within the ORF1b coding region of porcine astrovirus., PMID:36812864
Immunosensor for realtime monitoring of the expression of recombinant proteins during bioprocess., PMID:36716945
Charged Amino Acids Contribute to ZorO Toxicity., PMID:36668852
Generation of two CRISPR/Cas edited human induced pluripotent stem cell lines (DHMi005-A-3 and DHMi005-A-4) carrying a FLAG-tag after exon 9 of the TBX5 gene., PMID:36610218
Influence of affinity tags and tobacco PR1a signal peptide on detection, purification and bioactivity analyses of the small oomycete apoplastic effectors., PMID:36450976
Generation of Flag/DYKDDDDK Epitope Tag Knock-In Mice Using i-GONAD Enables Detection of Endogenous CaMKIIα and β Proteins., PMID:36233218
Generation of FLAG-tagged Arx knock-in mouse model., PMID:35656878
Identification, functional characterization and expression pattern of myeloid differentiation factor 88 (MyD88) in Nibea albiflora., PMID:35477097
Amber Suppression Technology for Mapping Site-specific Viral-host Protein Interactions in Mammalian Cells., PMID:35284605
Getting more out of FLAG-Tag co-immunoprecipitation mass spectrometry experiments using FAIMS., PMID:34990820
Demonstration of Co-Infection and Trans-Encapsidation of Viral RNA In Vitro Using Epitope-Tagged Foot-and-Mouth Disease Viruses., PMID:34960702
Insertion of Exogenous Genes within the ORF1a Coding Region of Porcine Astrovirus., PMID:34834925
Activity of the mouse Notch ligand DLL1 is sensitive to C-terminal tagging in vivo., PMID:34583743
Development and Validation of an ELISA for the Detection of Bluetongue Virus Serotype 4-Specific Antibodies., PMID:34578322
Structural basis of an epitope tagging system derived from Haloarcula marismortui bacteriorhodopsin I D94N and its monoclonal antibody GD-26., PMID:34499806
Virus-like particles with FLAG-tagged envelope protein as a tetravalent dengue vaccine candidate., PMID:34475493
Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells., PMID:34465007
Tandem Affinity Purification (TAP) of Low-Abundance Protein Complexes in Filamentous Fungi Demonstrated Using Magnaporthe oryzae., PMID:34236680
Mass Spectrometry-Based De Novo Sequencing of Monoclonal Antibodies Using Multiple Proteases and a Dual Fragmentation Scheme., PMID:34121409
Living-Cell Diffracted X-ray Tracking Analysis Confirmed Internal Salt Bridge Is Critical for Ligand-Induced Twisting Motion of Serotonin Receptors., PMID:34067933
Sulfation of a FLAG tag mediated by SLC35B2 and TPST2 affects antibody recognition., PMID:33951064
Vfr targets promoter of genes encoding methyl-accepting chemotaxis protein in Pseudomonas syringae pv. tabaci 6605., PMID:33659714
For research use only. Not for human or drug use.
