Anti-XRCC4 Antibody (R3S47)

Catalog No.

RHG52501

Species reactivity

Human, Mouse, Rat

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

IF: 1:50-1:200, IHC: 1:50-1:100, IP: 1:20, WB: 1:500-1:1000

Target

DNA repair protein XRCC4, X-ray repair cross-complementing protein 4, XRCC4

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q13426

Applications

IF, IHC, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3S47

X-ray scattering reveals disordered linkers and dynamic interfaces in complexes and mechanisms for DNA double-strand break repair impacting cell and cancer biology., PMID:34056803

Structural basis of long-range to short-range synaptic transition in NHEJ., PMID:33854234

Temporally uncoupled signal and coding joint formation in human V(D)J recombination., PMID:33157352

Genetic interaction between the non-homologous end-joining factors during B and T lymphocyte development: In vivo mouse models., PMID:32654175

Generation of a Mouse Model Lacking the Non-Homologous End-Joining Factor Mri/Cyren., PMID:31795137

Release of paused RNA polymerase II at specific loci favors DNA double-strand-break formation and promotes cancer translocations., PMID:31110352

Interstitial lung disease in Systemic sclerosis: insights into pathogenesis and evolving therapies., PMID:32185315

In cellulo phosphorylation of DNA double-strand break repair protein XRCC4 on Ser260 by DNA-PK., PMID:30247612

Effects of DNA end configuration on XRCC4-DNA ligase IV and its stimulation of Artemis activity., PMID:28696258

Decreased IL7Rα and TdT expression underlie the skewed immunoglobulin repertoire of human B-cell precursors from fetal origin., PMID:27658954

PAXX and XLF DNA repair factors are functionally redundant in joining DNA breaks in a G1-arrested progenitor B-cell line., PMID:27601633

Specific Roles of XRCC4 Paralogs PAXX and XLF during V(D)J Recombination., PMID:27601299

XLF deficiency results in reduced N-nucleotide addition during V(D)J recombination., PMID:27281794

Combined deletion of Xrcc4 and Trp53 in mouse germinal center B cells leads to novel B cell lymphomas with clonal heterogeneity., PMID:26740101

In cellulo phosphorylation of XRCC4 Ser320 by DNA-PK induced by DNA damage., PMID:26666690

Dynamic changes in subcellular localization of cattle XLF during cell cycle, and focus formation of cattle XLF at DNA damage sites immediately after irradiation., PMID:25947322

Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair., PMID:25941401

A recessive variant of XRCC4 predisposes to non- BRCA1/2 breast cancer in chinese women and impairs the DNA damage response via dysregulated nuclear localization., PMID:25360583

Patients with systemic sclerosis present increased DNA damage differentially associated with DNA repair gene polymorphisms., PMID:24488411

Polymorphisms in STK17A gene are associated with systemic lupus erythematosus and its clinical manifestations., PMID:23860322

Electrophoretic mobility shift assays for protein-DNA complexes involved in DNA repair., PMID:22941596

Preliminary study of thyroid and colon cancers-associated antigens and their cognate autoantibodies as potential cancer biomarkers., PMID:22612312

Impaired induction of DNA lesions during immunoglobulin class-switch recombination in humans influences end-joining repair., PMID:21135220

Widespread dependence of backup NHEJ on growth state: ramifications for the use of DNA-PK inhibitors., PMID:20950945

Alternative end-joining catalyzes class switch recombination in the absence of both Ku70 and DNA ligase 4., PMID:20142431

Alternative end-joining catalyzes robust IgH locus deletions and translocations in the combined absence of ligase 4 and Ku70., PMID:20133803

Mechanisms promoting translocations in editing and switching peripheral B cells., PMID:19587764

Immunology: B cells break the rules., PMID:19587755

Oncogenic transformation in the absence of Xrcc4 targets peripheral B cells that have undergone editing and switching., PMID:19064702

Efficiency of the DNA repair and polymorphisms of the XRCC1, XRCC3 and XRCC4 DNA repair genes in systemic lupus erythematosus., PMID:18852222

Mechanism and regulation of class switch recombination., PMID:18370922

Variation in DNA repair genes XRCC3, XRCC4, XRCC5 and susceptibility to myeloma., PMID:17901044

IgH class switching and translocations use a robust non-classical end-joining pathway., PMID:17713479

A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses., PMID:17396150

Severe combined immunodeficiency and microcephaly in siblings with hypomorphic mutations in DNA ligase IV., PMID:16358361

Radiosensitivity is predicted by DNA end-binding complex density, but not by nuclear levels of band components., PMID:15450732

Implication of DNA polymerase lambda in alignment-based gap filling for nonhomologous DNA end joining in human nuclear extracts., PMID:14561766

Biochemical evidence for Ku-independent backup pathways of NHEJ., PMID:12954774

In vitro and in vivo interactions of DNA ligase IV with a subunit of the condensin complex., PMID:12589063

Identification of human autoantibodies to the DNA ligase IV/XRCC4 complex and mapping of an autoimmune epitope to a potential regulatory region., PMID:12218164

Deficient nonhomologous end-joining activity in cell-free extracts from Brca1-null fibroblasts., PMID:12124328

Reconstitution of the mammalian DNA double-strand break end-joining reaction reveals a requirement for an Mre11/Rad50/NBS1-containing fraction., PMID:11809878

Autoantibodies against DNA double-strand break repair proteins., PMID:11689355

Genetic evidence for the involvement of DNA ligase IV in the DNA-PK-dependent pathway of non-homologous end joining in mammalian cells., PMID:11292837

Normal V(D)J recombination in cells from patients with Nijmegen breakage syndrome., PMID:11282395

A new gene involved in DNA double-strand break repair and V(D)J recombination is located on human chromosome 10p., PMID:10699181

Isolation of Ku70-binding proteins (KUBs)., PMID:10219089

Late embryonic lethality and impaired V(D)J recombination in mice lacking DNA ligase IV., PMID:9823897

A human severe combined immunodeficiency (SCID) condition with increased sensitivity to ionizing radiations and impaired V(D)J rearrangements defines a new DNA recombination/repair deficiency., PMID:9705945

Higher-order chromatin structure-dependent repair of DNA double-strand breaks: involvement of the V(D)J recombination double-strand break repair pathway., PMID:9588356

Document Download

Anti-XRCC4 Antibody (R3S47).pdf