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Anti-RAB25 Antibody (R3N70)

Catalog #:   RHF23401 Specific References (14) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: FCM, IF, IHC, WB
Accession: P57735
Overview

Catalog No.

RHF23401

Species reactivity

Human, Mouse

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Applications

FCM, IF, IHC, WB

Recommended Dilution

Application Dilution
FCM 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

RAB25, CATX8, CATX-8, Ras-related protein Rab-25

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P57735

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3N70

Note

For research use only.

Data Image
  • Flow Cytometry
    Flow cytometric analysis of NIH/3T3 cells using Rab25 mouse mAb (green) and negative control (purple).
  • Immunofluorescence
    Immunofluorescence analysis of A549 cells using RAB25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human esophagus tissues (left) and human lung cancer (right) using Rab25 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using Rab25 mouse mAb against MCF-7 (1), T47D (2) and GC7901 (3) cell lysate.
References

Two Rab proteins, vesicle-associated membrane protein 2 (VAMP-2) and secretory carrier membrane proteins (SCAMPs), are present on immunoisolated parietal cell tubulovesicles., PMID:9230141

Increased immunoreactivity for Rab11, a small GTP-binding protein, in low-grade dysplastic Barrett's epithelia., PMID:9389793

Association of Rab25 and Rab11a with the apical recycling system of polarized Madin-Darby canine kidney cells., PMID:9880326

Regulation of vesicle trafficking in madin-darby canine kidney cells by Rab11a and Rab25., PMID:10869360

Expression and properties of Rab25 in polarized Madin-Darby canine kidney cells., PMID:11210538

Brefeldin A rapidly disrupts plasma membrane polarity by blocking polar sorting in common endosomes of MDCK cells., PMID:11591819

Rab11b resides in a vesicular compartment distinct from Rab11a in parietal cells and other epithelial cells., PMID:14567990

Cholesterol-sensitive modulation of transcytosis., PMID:17392516

The recycling and transcytotic pathways for IgG transport by FcRn are distinct and display an inherent polarity., PMID:19451275

Rab25 and CLIC3 collaborate to promote integrin recycling from late endosomes/lysosomes and drive cancer progression., PMID:22197222

Epigenetic regulation of ZEB1-RAB25/ESRP1 axis plays a critical role in phenylbutyrate treatment-resistant breast cancer., PMID:26646320

Rab11-FIP1C Is a Critical Negative Regulator in ErbB2-Mediated Mammary Tumor Progression., PMID:26933086

Systematic investigation on the intracellular trafficking network of polymeric nanoparticles., PMID:28225130

An investigation into the effects of maternal supplementation with excess iodine on the mechanisms and impacts of reduced IgG absorption in the lamb postpartum., PMID:28443527

Datasheet

Document Download

Anti-RAB25 Antibody (R3N70).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-RAB25 Antibody (R3N70) [RHF23401]
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