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Anti-RAD52 Antibody (R3L11)

Catalog #:   RHE41301 Specific References (37) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, WB
Accession: P43351
Overview

Catalog No.

RHE41301

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, WB: 1:500-1:2000

Target

RAD52, DNA repair protein RAD52 homolog

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P43351

Applications

ELISA, FCM, IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3L11

Data Image
  • Flow Cytometry
    Flow cytometric analysis of MCF7 cells using RAD52 mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of HeLa cells using RAD52 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Western blot
    Western blot analysis using RAD52 mouse mAb against HepG2 (1), MCF-7 (2), MCF-7 (3), and C6 (4) cell lysate.
References

Transcriptomics and Proteomics Analysis of the Liver of RAD52 Knockout Mice., PMID:39796194

Rad52 mediates class-switch DNA recombination to IgD., PMID:35190531

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells., PMID:32597879

FKBP25 participates in DNA double-strand break repair., PMID:30620620

Rad52 competes with Ku70/Ku86 for binding to S-region DSB ends to modulate antibody class-switch DNA recombination., PMID:28176781

Effect of the expression of BRCA2 on spontaneous homologous recombination and DNA damage-induced nuclear foci in Saccharomyces cerevisiae., PMID:23328489

Sirtuin 6 (SIRT6) rescues the decline of homologous recombination repair during replicative senescence., PMID:22753495

Anti-angiogenic effects of the water extract of HangAmDan (WEHAD), a Korean traditional medicine., PMID:21416324

Unique DNA repair gene variations and potential associations with the primary antibody deficiency syndromes IgAD and CVID., PMID:20805886

Induction of apoptosis of Beta cells of the pancreas by advanced glycation end-products, important mediators of chronic complications of diabetes mellitus., PMID:19120318

BCR/ABL and other kinases from chronic myeloproliferative disorders stimulate single-strand annealing, an unfaithful DNA double-strand break repair., PMID:18757400

Distinct RAD51 associations with RAD52 and BCCIP in response to DNA damage and replication stress., PMID:18413737

Transcriptional profile of the homologous recombination machinery and characterization of the EhRAD51 recombinase in response to DNA damage in Entamoeba histolytica., PMID:18402694

Gross chromosomal rearrangements and elevated recombination at an inducible site-specific replication fork barrier., PMID:15935756

Proteomic changes during the B cell development., PMID:15652818

Immunoglobulin gene conversion: insights from bursal B cells and the DT40 cell line., PMID:14991701

In vivo assembly and disassembly of Rad51 and Rad52 complexes during double-strand break repair., PMID:14765116

Biochemical evidence for Ku-independent backup pathways of NHEJ., PMID:12954774

Characterization of the slow-growth phenotype of S. cerevisiae Whip/Mgs1 Sgs1 double deletion mutants., PMID:12509289

Differential effects of Rad52p overexpression on gene targeting and extrachromosomal homologous recombination in a human cell line., PMID:11809887

In vitro evidence for homologous recombinational repair in resistance to melphalan., PMID:11584063

Gain- and loss-of-function of Rhp51, a Rad51 homolog in fission yeast, reveals dissimilarities in chromosome integrity., PMID:11292845

Genetic evidence for the involvement of DNA ligase IV in the DNA-PK-dependent pathway of non-homologous end joining in mammalian cells., PMID:11292837

Mouse homolog of Saccharomyces cerevisiae spo11 is induced in normal mu(+)B-cells by stimuli that cause germline C(H) transcription and subsequent class switch recombination., PMID:10873300

Effects of mutations in DNA repair genes on formation of ribosomal DNA circles and life span in Saccharomyces cerevisiae., PMID:10207108

Visualisation of human rad52 protein and its complexes with hRad51 and DNA., PMID:9837724

Homologous recombination, but not DNA repair, is reduced in vertebrate cells deficient in RAD52., PMID:9774659

Rad51 immunocytology in rat and mouse spermatocytes and oocytes., PMID:9254722

The yeast HRS1 gene encodes a polyglutamine-rich nuclear protein required for spontaneous and hpr1-induced deletions between direct repeats., PMID:8849881

Gene conversion in the chicken immunoglobulin locus: a paradigm of homologous recombination in higher eukaryotes., PMID:8143801

Specific complex formation between proteins encoded by the yeast DNA repair and recombination genes RAD1 and RAD10., PMID:1518857

Evidence that an endo-exonuclease controlled by the NUC2 gene functions in the induction of 'petite' mutations in Saccharomyces cerevisiae., PMID:1657413

Genetic control of chromosome synapsis in yeast meiosis., PMID:2687110

An endo-exonuclease activity of yeast that requires a functional RAD52 gene., PMID:2830467

Purification and characterization of an endo-exonuclease from Saccharomyces cerevisiae that is influenced by the RAD52 gene., PMID:2826428

A yeast protein analogous to Escherichia coli RecA protein whose cellular level is enhanced after UV irradiation., PMID:3903433

DNA polymerases, deoxyribonucleases, and recombination during meiosis in Saccharomyces cerevisiae., PMID:6396507

Datasheet

Document Download

Anti-RAD52 Antibody (R3L11).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-RAD52 Antibody (R3L11) [RHE41301]
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