Overview
Catalog No.
RHE31201
Species reactivity
Human, Mouse, Monkey
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Applications
ELISA, FCM, IF, WB
Recommended Dilution
| Application |
Dilution |
| ELISA |
1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, WB: 1:500-1:2000 |
Target
TE2, hARD1, N-alpha-acetyltransferase 10, ARD1, ARD1A, NAA10, N-terminal acetyltransferase complex ARD1 subunit homolog A, NatA catalytic subunit Naa10
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P41227
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3K57
Note
For research use only.
Data Image
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Flow cytometric analysis of SMMC-7721 cells using NAA10 mouse mAb (green) and negative control (red).
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Immunofluorescence analysis of Hela cells using NAA10 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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Western blot analysis using NAA10 mouse mAb against COS7 (1), HEK293 (2), HL-60 (3), MCF-7 (4), Hela (5), NIH/3T3 (6), and C2C12 (7) cell lysate.
References
Expression of N-acetyl transferase human and human Arrest defective 1 proteins in thyroid neoplasms., PMID:16279846
Generation of novel monoclonal antibodies and their application for detecting ARD1 expression in colorectal cancer., PMID:18325661
[hARD1 antiserum preparation and primary immunohistochemical analysis of hARD1 in tumor tissues]., PMID:18837388
[Production of anti-recombinant human arrest defective 1 protein (hARD1) monoclonal antibodies for assaying human breast cancer tissues]., PMID:20353093
Peptide mimic isolated by autoantibody reveals human arrest defective 1 overexpression is associated with poor prognosis for colon cancer patients., PMID:20639454
Bioinformatics-Guided Identification and Quantification of Biomarkers of Crotalus atrox Envenoming and Its Neutralization by Antivenom., PMID:40147718
Datasheet