Catalog No.
RHD76102
Species reactivity
Human, Rat
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Applications
ELISA, IF, IHC, WB
Recommended Dilution
| Application |
Dilution |
| ELISA |
1:10000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000 |
Target
Low molecular mass protein 7, Macropain subunit C13, Really interesting new gene 10 protein, RING10, LMP7, Y2, Proteasome component C13, PSMB5i, Multicatalytic endopeptidase complex subunit C13, Proteasome subunit beta-5i, Proteasome subunit beta type-8, PSMB8
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P28062
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3H50
Note
For research use only.
Alternative exon usage and processing of the major histocompatibility complex-encoded proteasome subunits., PMID:1429565
Proteasome subunits encoded by the major histocompatibility complex are not essential for antigen presentation., PMID:1299222
Proteasome subunits encoded in the MHC are not generally required for the processing of peptides bound by MHC class I molecules., PMID:1436094
Delineation of the subunit composition of human proteasomes using antisera against the major histocompatibility complex-encoded LMP2 and LMP7 subunits., PMID:8278381
Proteasome subunits, low-molecular-mass polypeptides 2 and 7 are hyperexpressed by target cells in autoimmune thyroid disease but not in insulin-dependent diabetes mellitus: implications for autoimmunity., PMID:9271825
Intermediates in the formation of mouse 20S proteasomes: implications for the assembly of precursor beta subunits., PMID:9311996
Characterization of rabbit antisera elicited with human LMP2- and LMP7-specific peptides and recombinant proteins., PMID:9458109
Development and characterization of mouse anti-human LMP2, LMP7, TAP1 and TAP2 monoclonal antibodies., PMID:9510369
Invasion by Salmonella typhimurium induces increased expression of the LMP, MECL, and PA28 proteasome genes and changes in the peptide repertoire of HLA-B27., PMID:9746558
Up-regulation of the proteasome subunit LMP7 in tissues of endotoxemic rats., PMID:10779048
Gene transfer of human interferon gamma complementary DNA into a renal cell carcinoma line enhances MHC-restricted cytotoxic T lymphocyte recognition but suppresses non-MHC-restricted effector cell activity., PMID:10849555
Tissue distribution of constitutive proteasomes, immunoproteasomes, and PA28 in rats., PMID:11032729
Association of immunoproteasomes with the endoplasmic reticulum., PMID:11104664
IFN-beta mediates coordinate expression of antigen-processing genes in RSV-infected pulmonary epithelial cells., PMID:11159003
TAP1 down-regulation in primary melanoma lesions: an independent marker of poor prognosis., PMID:11241306
Accessory proteins that control the assembly of MHC molecules with peptides., PMID:11444385
Introduction of tapasin gene restores surface expression of HLA class I molecules, but not antigen presentation of an HIV envelope peptide in a hepatoma cell line., PMID:11444872
Molecular studies and NK cell function of a new case of TAP2 homozygous human deficiency., PMID:11529920
Bipartite regulation of different components of the MHC class I antigen-processing machinery during dendritic cell maturation., PMID:11717192
Total loss of MHC class I in colorectal tumors can be explained by two molecular pathways: beta2-microglobulin inactivation in MSI-positive tumors and LMP7/TAP2 downregulation in MSI-negative tumors., PMID:12694570
IFN-alpha mediates the up-regulation of HLA class I on melanoma cells without switching proteasome to immunoproteasome., PMID:14645150
Neuronal induction of the immunoproteasome in Huntington's disease., PMID:14684867
Antigen-processing machinery in human dendritic cells: up-regulation by maturation and down-regulation by tumor cells., PMID:15265880
Coordinated downregulation of the antigen presentation machinery and HLA class I/beta2-microglobulin complex is responsible for HLA-ABC loss in bladder cancer., PMID:15455355
Hepatocytes express abundant surface class I MHC and efficiently use transporter associated with antigen processing, tapasin, and low molecular weight polypeptide proteasome subunit components of antigen processing and presentation pathway., PMID:16002705
Development and characterization of human constitutive proteasome and immunoproteasome subunit-specific monoclonal antibodies., PMID:16101829
Human leukocyte antigen and antigen processing machinery component defects in astrocytic tumors., PMID:16322289
Proteasomes shape the repertoire of T cells participating in antigen-specific immune responses., PMID:16549793
The ubiquitin-proteasome system plays essential roles in presenting an 8-mer CTL epitope expressed in APC to corresponding CD8+ T cells., PMID:16569681
Autoimmune reactivity against the 20S-proteasome includes immunosubunits LMP2 (beta1i), MECL1 (beta2i) and LMP7 (beta5i)., PMID:18375405
[Immune proteasomes in the developing rat spleen]., PMID:18949853
Age-related increase in the immunoproteasome content in rat hippocampus: molecular and functional aspects., PMID:19012754
Down-regulation of proteasomal subunit MB1 is an independent predictor of improved survival in ovarian cancer., PMID:19243813
A novel role for the immunoproteasome in retinal function., PMID:20881299
The immunoproteasome in steatohepatitis: its role in Mallory-Denk body formation., PMID:21256843
A putative role for the immunoproteasome in the maintenance of pluripotency in human embryonic stem cells., PMID:22532526
Rescue of MHC-1 antigen processing machinery by down-regulation in expression of IGF-1 in human glioblastoma cells., PMID:23526983
Can tonsillectomy modify the innate and adaptive immunity pathways involved in IgA nephropathy?, PMID:24756968
ONX-0914, a selective inhibitor of immunoproteasome, ameliorates experimental autoimmune myasthenia gravis by modulating humoral response., PMID:28844501
Immunoproteasome inhibition prevents chronic antibody-mediated allograft rejection in renal transplantation., PMID:29229189
Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens., PMID:30343902
Whole exome sequencing approach to childhood onset familial erythrodermic psoriasis unravels a novel mutation of CARD14 requiring unusual high doses of ustekinumab., PMID:31286971
Overexpression of immunoproteasome low-molecular-mass polypeptide 7 and inhibiting role of next-generation proteasome inhibitor ONX 0912 on cell growth in glioma., PMID:31503210
Proteasomal adaptations underlying carfilzomib-resistance in human bone marrow plasma cells., PMID:31595669
Bioinformatics Analysis of Potential Key Genes in Trastuzumab-Resistant Gastric Cancer., PMID:31949544
Myositis with sarcoplasmic inclusions in Nakajo-Nishimura syndrome: a genetic inflammatory myopathy., PMID:32144790
Efficacy, safety, and molecular response predictors of oral ixazomib and short-course rituximab in untreated iNHL., PMID:36385536
Immunohistochemical Identification and Assessment of the Location of Immunoproteasome Subunits LMP2 and LMP7 in Acquired Cholesteatoma., PMID:37762439
For research use only. Not for human or drug use.
