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Anti-RPA2 Antibody (R3E18)

Catalog #:   RHD14102 Specific References (22) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: FCM, IHC, WB
Accession: P15927
Overview

Catalog No.

RHD14102

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

FCM: 1:200-1:400, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

RPA34, Replication protein A 34 kDa subunit, Replication protein A 32 kDa subunit, RF-A protein 2, RP-A p34, Replication factor A protein 2, REPA2, RPA2, RPA32, RP-A p32

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P15927

Applications

FCM, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3E18

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using RFA2 mouse mAb (green) and negative control (red).
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human liver tissues using RFA2 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using RFA2 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using RFA2 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using RFA2 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using RFA2 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using RFA2 mouse mAb against Hela (1), MCF-7 (2), T47D (3), Ramos (4), HEK293 (5), HepG2 (6) and A431 (7) cell lysate.
References

Replication Protein A (RPA1, RPA2 and RPA3) expression in gastric cancer: correlation with clinicopathologic parameters and patients' survival., PMID:32862594

Pea (Pisum sativum) allergy in children: Pis s 1 is an immunodominant major pea allergen and presents IgE binding sites with potential diagnostic value., PMID:32078204

PBMCs transcriptome profiles identified breed-specific transcriptome signatures for PRRSV vaccination in German Landrace and Pietrain pigs., PMID:31536525

Author Correction: Per2 induction limits lymphoid-biased haematopoietic stem cells and lymphopoiesis in the context of DNA damage and ageing., PMID:30808996

Identification of stroke-associated-antigens via screening of recombinant proteins from the human expression cDNA library (SEREX)., PMID:25890248

Leucine methylation of protein phosphatase PP4C at C-terminal is critical for its cellular functions., PMID:25130464

Interplay of DNA damage and cell cycle signaling at the level of human replication protein A., PMID:25091156

Activation of WIP1 phosphatase by HTLV-1 Tax mitigates the cellular response to DNA damage., PMID:23405243

Protein interaction and cellular localization of human CDC45., PMID:23364835

Parvovirus B19 infection of human primary erythroid progenitor cells triggers ATR-Chk1 signaling, which promotes B19 virus replication., PMID:21680529

Analysis of protein phosphorylation in cisplatin-treated human cells following annexin V-based separation and multi-antibody screening., PMID:20952762

A PP4 phosphatase complex dephosphorylates RPA2 to facilitate DNA repair via homologous recombination., PMID:20154705

Ionizing radiation-dependent and independent phosphorylation of the 32-kDa subunit of replication protein A during mitosis., PMID:19671522

Immunolocalization of Kim-1, RPA-1, and RPA-2 in kidney of gentamicin-, mercury-, or chromium-treated rats: relationship to renal distributions of iNOS and nitrotyrosine., PMID:18441258

NBS1 mediates ATR-dependent RPA hyperphosphorylation following replication-fork stall and collapse., PMID:18003706

Sequential and synergistic modification of human RPA stimulates chromosomal DNA repair., PMID:17928296

Functional characterization of replication protein A2 (RPA2) from Cryptosporidium parvum., PMID:15133081

Replication protein A (RPA) phosphorylation prevents RPA association with replication centers., PMID:14966274

The second largest subunit of Trypanosoma brucei's multifunctional RNA polymerase I has a unique N-terminal extension domain., PMID:12615318

The 32-kilodalton subunit of replication protein A interacts with menin, the product of the MEN1 tumor suppressor gene., PMID:12509449

Heterogeneous expression and functional analysis of two distinct replication protein A large subunits from Cryptosporidium parvum., PMID:12392913

RPA-2.10: an anti-CD2 monoclonal antibody that inhibits alloimmune responses and monitors T cell activation., PMID:2908548

Datasheet

Document Download

Anti-RPA2 Antibody (R3E18).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-RPA2 Antibody (R3E18) [RHD14102]
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