Catalog No.
YHD31301
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Gly484-Ile663
Predicted molecular weight
22.90 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P18858
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
DNA ligase 1, Polydeoxyribonucleotide synthase [ATP] 1, DNA ligase I, LIG1
Joining of DNA breaks- interplay between DNA ligases and poly (ADP-ribose) polymerases., PMID:40347914
Prime Editing in Dividing and Quiescent Cells., PMID:40332080
Molecular basis for RNA discrimination by human DNA ligase 1., PMID:40239996
Comprehensive interrogation of synthetic lethality in the DNA damage response., PMID:40205037
Identification of potent multi-target antiviral natural compounds from the fungal metabolites against aspartyl viral polymerases., PMID:40082688
Repair pathway coordination from gap filling by polβ and subsequent nick sealing by LIG1 or LIG3α governs BER efficiency at the downstream steps., PMID:40081282
LIG1 Is a Synthetic Lethal Target in BRCA1 Mutant Cancers., PMID:39868490
Impact of DNA Ligase 1 Genotypes on Childhood Acute Lymphocytic Leukemia., PMID:39740875
CRISPR/Cas9 screens identify LIG1 as a sensitizer of PARP inhibitors in castration-resistant prostate cancer., PMID:39718835
DNA Ligase I Circularises Potato Spindle Tuber Viroid RNA in a Biomolecular Condensate., PMID:39715063
A Commentary on Mitochondrial Dysfunction and Compromised DNA Repair in Neurodegeneration: The Emerging Role of FUS in ALS., PMID:39679063
Decoding protein dynamicity in DNA ligase activity through deep learning-based structural ensembles., PMID:39574676
Rare variants of DNA ligase 1 show distinct mechanisms of deficiency., PMID:39510190
Probing the mechanism of nick searching by LIG1 at the single-molecule level., PMID:39404052
CDK-dependent phosphorylation regulates PNKP function in DNA replication., PMID:39395804
Inactive Parp2 causes Tp53-dependent lethal anemia by blocking replication-associated nick ligation in erythroblasts., PMID:39383878
High fidelity DNA ligation prevents single base insertions in the yeast genome., PMID:39379399
Mechanistic Basis for a Single Amino Acid Residue Mutation Causing Human DNA Ligase 1 Deficiency, A Rare Pediatric Disease., PMID:39374888
LIG1 is a novel marker for bladder cancer prognosis: evidence based on experimental studies, machine learning and single-cell sequencing., PMID:39234248
miR-325 Supresses Cell Proliferation and Migration in Non-Small Cell Lung Cancer via Targeting DNA Ligase 1 (LIG1)., PMID:39231317
Structures of LIG1 uncover the mechanism of sugar discrimination against 5'-RNA-DNA junctions during ribonucleotide excision repair., PMID:39159820
Redundant but essential functions of PARP1 and PARP2 in DNA ligase I-independent DNA replication., PMID:39106163
Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds., PMID:38963754
Alteration of N6-methyladenosine modification profiles in the neutrophilic RNAs following ischemic stroke., PMID:38945353
Severe Combined Immunodeficiency from a Homozygous DNA Ligase 1 Mutant with Reduced Catalytic Activity but Increased Ligation Fidelity., PMID:38896336
The TIMELESS and PARP1 interaction suppresses replication-associated DNA gap accumulation., PMID:38801073
Therapeutic validation of MMR-associated genetic modifiers in a human ex vivo model of Huntington disease., PMID:38749429
Impact of DNA ligase inhibition on the nick sealing of polβ nucleotide insertion products at the downstream steps of base excision repair pathway., PMID:38736258
Impact of DNA ligase 1 and IIIα interactions with APE1 and polβ on the efficiency of base excision repair pathway at the downstream steps., PMID:38718860
Investigating Potential Cancer Therapeutics: Insight into Histone Deacetylases (HDACs) Inhibitions., PMID:38675404
Uncovering nick DNA binding by LIG1 at the single-molecule level., PMID:38586032
Inactive Parp2 causes Tp53-dependent lethal anemia by blocking replication-associated nick ligation in erythroblasts., PMID:38559022
Structures of LIG1 provide a mechanistic basis for understanding a lack of sugar discrimination against a ribonucleotide at the 3'-end of nick DNA., PMID:38522520
Unfilled gaps by polβ lead to aberrant ligation by LIG1 at the downstream steps of base excision repair pathway., PMID:38366780
Synergy between human DNA ligase I and topoisomerase 1 unveils new therapeutic strategy for the management of colorectal cancer., PMID:38179981
hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma., PMID:38044421
Single-cell transcriptomics reveals subtype-specific molecular profiles in Nrf2-deficient macrophages from murine atherosclerotic aortas., PMID:37965327
Identification of DNA damage response-related genes as biomarkers for castration-resistant prostate cancer., PMID:37950047
Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy., PMID:37873068
DNA Damage Induced by T-2 Mycotoxin in Human Skin Fibroblast Cell Line-Hs68., PMID:37833905
Mammalian DNA ligases; roles in maintaining genome integrity., PMID:37714297
Single-Nucleotide Polymorphisms in Base-Excision Repair-Related Genes Involved in the Risk of an Occurrence of Non-Alcoholic Fatty Liver Disease., PMID:37511066
In Silico Characterization of RNASEH2A Pathogenic Variants and Identification of Novel Splice Site Donor Variant c.549+1G>T in Indian Population., PMID:37456470
The Association of DNA Ligase 1 Rs20579 Polymorphism With Lung Cancer Risk Among Taiwanese., PMID:37369482
Explore the dominant factor in prime editing via a view of DNA processing., PMID:37325180
The Curcumin Analog PAC Is a Potential Solution for the Treatment of Triple-Negative Breast Cancer by Modulating the Gene Expression of DNA Repair Pathways., PMID:37298600
PCNA Ser46-Leu47 residues are crucial in preserving genomic integrity., PMID:37205694
Diagnosing mitochondrial, neurogastrointestinal leukoencephalopathy requires mutations in TYMP1, POLG1, LIG1, or RRM2B., PMID:37055871
Functional hierarchy of PCNA-interacting motifs in DNA processing enzymes., PMID:37039277
[Regulatory Potential of SNP Markers in Genes of DNA Repair Systems]., PMID:36976737
For research use only. Not for human or drug use.
