Catalog No.
RHA56303
Species reactivity
Human, Mouse, Rat, Monkey
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:50-1:200, IHC: 1:200-1:1000, WB: 1:500-1:2000
Target
PDK1, hPDK1, PDPK1, 3-phosphoinositide-dependent protein kinase 1
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
O15530
Applications
ELISA, FCM, IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R2U74
Hypoxia inducible factor-1α drives cancer resistance to cuproptosis., PMID:40054467
NIR-II Imaging for Tracking the Spatiotemporal Immune Microenvironment in Atherosclerotic Plaques., PMID:39630481
Leucine-Rich Alpha-2-Glycoprotein 1 Promotes Metastatic Colorectal Cancer Growth Through Human Epidermal Growth Factor Receptor 3 Signaling., PMID:39393543
O-GlcNAcylation mediates Wnt-stimulated bone formation by rewiring aerobic glycolysis., PMID:39256595
Retraction Note: Long non-coding RNA FAL1 regulated cell proliferation through Akt pathway via targeting PDK1 in esophageal cancer cells., PMID:38305630
A PD-L1 Antibody-Conjugated PAMAM Dendrimer Nanosystem for Simultaneously Inhibiting Glycolysis and Promoting Immune Response in Fighting Breast Cancer., PMID:37522451
PD-L1 expression is regulated by ATP-binding of the ERBB3 pseudokinase domain., PMID:37397533
Phosphoinositol 3-kinase-driven NET formation involves different isoforms and signaling partners depending on the stimulus., PMID:36761736
Molecular classification of human papillomavirus-positive cervical cancers based on immune signature enrichment., PMID:36203656
Metabolic regulation of intrasynovial flexor tendon repair: The effects of dichloroacetate administration on early tendon healing in a canine model., PMID:35488732
GDC-0941 activates integrin linked kinase (ILK) expression to cause resistance to GDC-0941 in breast cancer by the tumor necrosis factor (TNF)-α signaling pathway., PMID:35477364
Patient pIgR-enriched extracellular vesicles drive cancer stemness, tumorigenesis and metastasis in hepatocellular carcinoma., PMID:34922977
Hypoxia, Metabolic Reprogramming, and Drug Resistance in Liver Cancer., PMID:34359884
Small molecules targeting the innate immune cGAS‒STING‒TBK1 signaling pathway., PMID:33354501
Up-regulation of FoxO1 contributes to adverse vascular remodelling in type 1 diabetic rats., PMID:33108705
CRISPR-Cas13d for Gene Knockdown and Engineering of CHO Cells., PMID:32911927
Cancer-derived IgG involved in cisplatin resistance through PTP-BAS/Src/PDK1/AKT signaling pathway., PMID:32730654
Systems analysis of protein signatures predicting cetuximab responses in KRAS, NRAS, BRAF and PIK3CA wild-type patient-derived xenograft models of metastatic colorectal cancer., PMID:32700762
Glucocorticoids and serum- and glucocorticoid-inducible kinase 1 are potent regulators of CFTR in the native intestine: implications for stress-induced diarrhea., PMID:32567324
Yokukansan, a Kampo medicine, enhances the level of neuronal lineage markers in differentiated P19 embryonic carcinoma cells., PMID:31692643
Aberrant upregulation of PDK1 in ovarian cancer cells impairs CD8+ T cell function and survival through elevation of PD-L1., PMID:31646108
Rational combination with PDK1 inhibition overcomes cetuximab resistance in head and neck squamous cell carcinoma., PMID:31578313
Associations of PGK1 promoter hypomethylation and PGK1-mediated PDHK1 phosphorylation with cancer stage and prognosis: a TCGA pan-cancer analysis., PMID:31578148
The AKT kinase signaling network is rewired by PTEN to control proximal BCR signaling in germinal center B cells., PMID:31011187
Stage-specific requirement of kinase PDK1 for NK cells development and activation., PMID:30622306
Spatial alterations of De Novo purine biosynthetic enzymes by Akt-independent PDK1 signaling pathways., PMID:29668719
JAM3 maintains leukemia-initiating cell self-renewal through LRP5/AKT/β-catenin/CCND1 signaling., PMID:29584620
Assessment of PI3K/AKT/PTEN signaling pathway activity in colorectal cancer using quantum dot-conjugated antibodies., PMID:29422975
SHORT syndrome due to a novel de novo mutation in PRKCE (Protein Kinase Cɛ) impairing TORC2-dependent AKT activation., PMID:28934384
Inhibition of SHIP2 in CD2AP-deficient podocytes ameliorates reactive oxygen species generation but aggravates apoptosis., PMID:28878342
Particular phosphorylation of PI3K/Akt on Thr308 via PDK-1 and PTEN mediates melatonin's neuroprotective activity after focal cerebral ischemia in mice., PMID:28395173
Increased expression of platelet-derived growth factor associated protein-1 is associated with PDGF-B mediated glioma progression., PMID:27448842
Chromatin remodeling gene AT-rich interactive domain-containing protein 1A suppresses gastric cancer cell proliferation by targeting PIK3CA and PDK1., PMID:27323812
Dynamic Contrast Enhanced MRI Assessing the Antiangiogenic Effect of Silencing HIF-1α with Targeted Multifunctional ECO/siRNA Nanoparticles., PMID:27264671
Prevention Effects and Possible Molecular Mechanism of Mulberry Leaf Extract and its Formulation on Rats with Insulin-Insensitivity., PMID:27054886
S6 kinase phosphorylated at T229 is involved in tau and actin pathologies in Alzheimer's disease., PMID:26582459
Double-Edge Sword of Sustained ROCK Activation in Prion Diseases through Neuritogenesis Defects and Prion Accumulation., PMID:26241960
[Metabolism enzyme controls cancer stemness]., PMID:25985625
Comprehensive analysis of glycolytic enzymes as therapeutic targets in the treatment of glioblastoma., PMID:25932951
Similar PDK1-AKT-mTOR pathway activation in balloon cells and dysmorphic neurons of type II focal cortical dysplasia with refractory epilepsy., PMID:25847349
Targeting pyruvate kinase M2 contributes to radiosensitivity of non-small cell lung cancer cells in vitro and in vivo., PMID:25444918
Protein kinase C in the wood frog, Rana sylvatica: reassessing the tissue-specific regulation of PKC isozymes during freezing., PMID:25210662
PDK1 regulates B cell differentiation and homeostasis., PMID:24979759
Effects of resveratrol on the insulin signaling pathway of obese mice., PMID:24675832
Increased 8-hydroxy-2'-deoxyguanosine in plasma and decreased mRNA expression of human 8-oxoguanine DNA glycosylase 1, anti-oxidant enzymes, mitochondrial biogenesis-related proteins and glycolytic enzymes in leucocytes in patients with systemic lupus erythematosus., PMID:24345202
Isoaspartate accumulation in mouse brain is associated with altered patterns of protein phosphorylation and acetylation, some of which are highly sex-dependent., PMID:24224061
Thyrotropin regulates IL-6 expression in CD34+ fibrocytes: clear delineation of its cAMP-independent actions., PMID:24086448
Characterization of fragmented 3-phosphoinsitide-dependent protein kinase-1 (PDK1) by phosphosite-specific antibodies., PMID:24044887
Mammalian target of rapamycin pathway activity in alveolar soft part sarcoma., PMID:23871289
Crosstalk between PI3 kinase/PDK1/Akt/Rac1 and Ras/Raf/MEK/ERK pathways downstream PDGF receptor., PMID:23817184
For research use only. Not for human or drug use.
