Catalog No.
KVV08902
Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for HPV16 E7 has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any HPV16 E7 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for HPV16 E7 is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HPV16 E7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of HPV16 E7 concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative (Sandwich)
Range
93.75 - 6,000 pg/mL
Sensitivity
37.98 pg/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
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1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
16
|
16
|
16
|
24
|
24
|
24
|
|
Mean (pg/mL)
|
2412.7
|
682.0
|
184.5
|
2417.0
|
633.3
|
158.8
|
|
Standard deviation
|
187.6
|
42.2
|
14.0
|
211.4
|
27.4
|
19.8
|
|
CV (%)
|
7.8
|
6.2
|
7.6
|
8.7
|
4.3
|
12.5
|
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Alternative Names
E7, ProteinE7
Generation of a spontaneous murine HPV + oral cancer model with site-specific oncogene insertion using CRISPR-SONIC., PMID:40533862
Novel biomarker discovery-coupled plasma therapy with enhanced tumor penetration for non-surgical treatment of cervical cancer., PMID:40505890
HPV16 E7 Enhances Cell Stemness via RTKN2-Mediated Activation of the NF-κB Pathway in Cervical Cancer., PMID:40495058
A novel Zn2+ probe for fluorescent monitoring of drug and E6/E7 protein interaction in living cells., PMID:40480729
Crosstalk Between the Oncoproteins of High-Risk Human Papillomaviruses Types 16 and 18 in Colorectal Cancer Cell Models., PMID:40468102
Therapeutic mRNA vaccines targeting human papillomavirus type 16 E7 protein demonstrate significant antitumor efficacy in murine models of HPV-associated tumors., PMID:40446873
A Luminescence-Based Method for In Vitro Screening of Immunomodulatory Checkpoints and Therapeutics., PMID:40402459
Zinc-alpha-2-glycoprotein overexpression and maintaining anti-apoptotic function in oral squamous cell carcinoma., PMID:40398100
Higher Expression of HPV16 Derived E7_LI Transcript Observed in Men With HIV and Recurrent Anal Cancer., PMID:40317526
Thymoquinone enhances efficacy of cervical Cancer therapeutic vaccines via modulating CD8+ T cells., PMID:40316484
HPV16 E7 inhibits HBD2 expression by down-regulation of ASK1-p38 MAPK pathway in cervical cancer., PMID:40253372
E7HPV16 Oncogene and 17beta-Estradiol Stress, Promotes Oncogenic microRNA Expression Patterns, Cell Proliferation and Cervical Intraepithelial Neoplasia 1., PMID:40114417
Metformin-induced E6/E7 inhibition prevents HPV-positive cancer progression through p53 reactivation., PMID:40100964
[Redox Status and Protein Glutathionylation in Binase-Treated HPV16-Positive SiHa Carcinoma Cells]., PMID:39970121
Structural engineering of stabilized, expanded epitope nanoparticle vaccines for HPV., PMID:39958352
Immunization with recombinant HPV16-E7d in fusion with Flagellin as a cancer vaccine: Effect of antigen-adjuvant orientation on the immune response pattern., PMID:39939497
Development and evaluation of a novel E7 multi-epitopic vaccine for human papillomavirus type 16: design, expression, purification, and immunological characterization., PMID:39910471
The combined immunization of cervical cancer therapeutic vaccine based on Listeria balanced lethal system has a significant therapeutic effect on tumor model mice., PMID:39870008
An exploration of the natural and acquired immunological mechanisms to high-risk human papillomavirus infection and unmasking immune escape in cervical cancer: A concise synopsis., PMID:39850385
Oncoproteins E6/E7 of the human papillomavirus types 16 & 18 synergize in modulating oncogenes and tumor suppressor proteins in colorectal cancer., PMID:39815804
Roles of Mature Domain Targeting Signals (MTSs) for Protein Translocation and Secretion in Lactococcus lactis., PMID:39796073
Augmented immunogenicity of the HPV16 DNA vaccine via dual adjuvant approach: integration of CpG ODN into plasmid backbone and co-administration with IL-28B gene adjuvant., PMID:39780219
SLC7A11 Expression Is Up-Regulated in HPV- and Tobacco-Associated Lung Cancer., PMID:39769017
CRISPR/Cas14a integrated with DNA walker based on magnetic self-assembly for human papillomavirus type 16 oncoprotein E7 ultrasensitive detection., PMID:39764982
Designing of an mRNA vaccine against high-risk human papillomavirus targeting the E6 and E7 oncoproteins exploiting immunoinformatics and dynamic simulation., PMID:39761277
Characterization of the adaptive immune response in a mouse model for HPV-positive head and neck squamous cell carcinoma with implications to human disease., PMID:39751916
Comparative efficacy of HPV 16/18 DNA and E6/E7 mRNA testing in detecting high-grade cervical lesions (CIN2+) in women with cervical biopsies., PMID:39733635
Entropy-Driven Molecular Beacon Assisted Special RCA Assay with Enhanced Sensitivity for Room Temperature DNA Biosensing., PMID:39727883
[Amino Acid Substitution Patterns in the E6 and E7 Proteins of HPV Type 16: Phylogeography and Evolution]., PMID:39709561
LADS: a powerful vaccine platform for cancer immunotherapy and prevention., PMID:39696249
Upregulation of HPV16E1 and E7 expression and FOXO3a mRNA downregulation in high-grade cervical neoplasia., PMID:39655333
The HPV101 E7 protein shares host cellular targets and biological activities with high-risk HPV16 E7., PMID:39643241
STING Nanoagonist Boosts Antitumor Immunity of Therapeutic DNA Vaccines., PMID:39621441
Squamous cell carcinoma is associated with reduced IL34 expression, alterations in the Langerhans cell antigen-processing-presentation machinery and poor patient survival., PMID:39619016
Human Papillomavirus Infection and Autoimmune Diseases: A Two-Sample Bidirectional Mendelian Randomization Study., PMID:39618109
Development of a magnetic α-Fe2O3/Fe3O4 heterogeneous nanorod-based electrochemical biosensing platform for HPV16 E7 oncoprotein detection., PMID:39603292
ELF3 Overexpression Contributes to the Malignant Transformation of HPV16 E6/E7-Immortalized Keratinocytes by Promoting CCNE2 Expression., PMID:39572025
LAMP-based electrochemical platform for monitoring HPV genome integration at the mRNA level associated with higher risk of cervical cancer progression., PMID:39420658
Detection of transcriptionally active high-risk human papillomavirus in patients with oesophageal carcinoma by real-time PCR., PMID:39412909
Recombinant adenoviruses expressing HPV16/18 E7 upregulate the HDAC6 and DNMT3B genes in C33A cells., PMID:39411320
HPV16 mutant E6/E7 construct is protective in mouse model., PMID:39350162
Epstein-Barr virus replication within differentiated epithelia requires pRb sequestration of activator E2F transcription factors., PMID:39291960
hnRNP H controls alternative splicing of human papillomavirus type 16 E1, E6, E7, and E6^E7 mRNAs via GGG motifs., PMID:39287390
The lncRNA and miRNA regulatory axis in HPV16-positive oropharyngeal cancers., PMID:39244802
HPV 16/18 E7 oncoprotein detection as a promising triage strategy for HPV 16/18-positive patients: A prospective multicenter study with a 2-year follow up., PMID:39244722
No genetic causal association between human papillomavirus and lung cancer risk: a bidirectional two-sample Mendelian randomization analysis., PMID:39227966
Specific targeting of cancer vaccines to antigen-presenting cells via an endogenous TLR2/6 ligand derived from cysteinyl-tRNA synthetase 1., PMID:39066478
Differential tumor immune microenvironment coupled with tumor progression or tumor eradication in HPV-antigen expressing squamous cell carcinoma (SCC) models., PMID:39055715
Time to take HPV infection in colorectal cancer patients more seriously., PMID:39050539
Quantification of HPV16 E7 Oncoproteins in Urine Specimens from Women with Cervical Intraepithelial Neoplasia., PMID:38930587
For research use only. Not for human or drug use.
