Catalog No.
KGP13002
Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for HEK293 HCP has been pre-coated onto a microplate. Standards or samples and a biotin-labeled antibody specific for HEK293 HCP are pipetted into the wells, standards or HEK 293 HCP in the samples form immunocomplex with immobilized antibody and biotinylated anti-HEK 293 HCP antibody. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HEK293 HCP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Specificity
HEK293 Host Cell Proteins (HCP)
Applications
Used for the quantitative determination of HEK293 HCP concentration in biological products derived from HEK 293 and HEK 293F cell lines.
Detection method
Colorimetric
Sample type
HEK293 HCP impurities in samples.
Assay type
Quantitative (Sandwich)
Range
10.24 - 1,000 ng/mL
Sensitivity
5 ng/ml
Precision
Intra-Assay Precision (Precision within an assay): <20%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <20%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
Recovery
70%-130%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Alternative Names
HEK293 Host Cell Proteins (HCP)
HIV Vpr activates a nucleolar-specific ATR pathway to degrade the nucleolar stress sensor CCDC137., PMID:40530693
Dual-Luciferase Reporter Assay to Investigate Virus-Host Interactions., PMID:40515924
Determination of Protein Ubiquitination After Protein Denaturation in Virus-Host Interactions., PMID:40515897
Investigation of Protein Fatty Acylation in Virus-Host Interaction., PMID:40515896
Stem loop binding protein promotes SARS-CoV-2 replication via -1 programmed ribosomal frameshifting., PMID:40514371
Study on real time response of live cells to lentivirus infection monitored by Raman spectroscopy., PMID:40483793
Wisteria floribunda agglutinin enhances Zaire ebolavirus entry through interactions at specific N-linked glycosylation sites on the virus glycoprotein complex., PMID:40476860
Inhibition of virally induced TFEB proteasomal degradation as a host-centric therapeutic approach for coronaviral infection., PMID:40465712
Scalable Production of Recombinant Vesicular Stomatitis Virus Pseudoparticles Using HEK293 Suspension Cultures., PMID:40462709
SFTSV NSs degrades SAFA via autophagy to suppress SAFA-dependent antiviral response., PMID:40460159
Midnolin inhibits coronavirus proliferation by degrading viral proteins., PMID:40439407
ACE2 from Pipistrellus abramus bats is a receptor for HKU5 coronaviruses., PMID:40436893
A Novel Antiviral Therapeutic Platform: Anchoring IFN-β to the Surface of Infectious Virions Equips Interferon-Evasive Virions with Potent Antiviral Activity., PMID:40431708
Identification of TRIM21 and TRIM14 as Antiviral Factors Against Langat and Zika Viruses., PMID:40431659
Host susceptibilities and entry processes of SARS-CoV-2 Omicron variants using pseudotyped viruses carrying spike protein., PMID:40426227
Immune evasion, infectivity, and membrane fusion of the SARS-CoV-2 JN.1 variant., PMID:40413500
HERC5-mediated ISGylation of SARS-CoV-2 nsp8 facilitates its degradation and inhibits viral replication., PMID:40409630
The viral early protein 4 of human papillomavirus type 16 suppresses innate antiviral immunity., PMID:40409624
Kaposi's sarcoma-associated herpesvirus induces mitochondrial fission to evade host immune responses and promote viral production., PMID:40404827
Cullin 3-mediated ubiquitination restricts enterovirus D68 replication and is counteracted by viral protease 3C., PMID:40396757
Retroviral adapters hijack the RNA helicase UPF1 in a CRM1/XPO1-dependent manner and reveal proviral roles of UPF1., PMID:40396490
A comparison of SWATH-MS methods for measurement of residual host cell proteins in adeno-associated virus preparations., PMID:40386465
An integrated proteomics approach identifies phosphorylation sites on viral and host proteins that regulate West Nile virus infection., PMID:40381193
PRMT3 reverses HIV-1 latency by increasing chromatin accessibility to form a TEAD4-P-TEFb-containing transcriptional hub., PMID:40374607
Encephalomyocarditis virus non-structural protein 2C induces the degradation of NDP52 autophagy protein to promote its own survival., PMID:40373618
ANP32 proteins from ticks and vertebrates are key host factors for replication of Bourbon virus across species., PMID:40366164
Zebrafish TRIM2a promotes virus replication via ubiquitination of IRF3 and autophagic cargo receptor p62., PMID:40359380
Assessment of the Phenolics Content in Epilobium angustifolium and Epilobium hirsutum Extracts and Their Pharmacological Activity., PMID:40359335
SARS-CoV-2 nsp1 mediates broad inhibition of translation in mammals., PMID:40359110
SARS-CoV-2 nsp16 is regulated by host E3 ubiquitin ligases, UBR5 and MARCHF7., PMID:40358464
Determinants of susceptibility to SARS-CoV-2 infection in murine ACE2., PMID:40353671
Cellular transcription factor TFII-I represses adenovirus gene expression., PMID:40353670
Design, synthesis and biological evaluation of dapsone derivatives with broad-spectrum antiviral activity., PMID:40344737
DEAD-box RNA helicase 10 inhibits porcine circovirus type 3 replication by interacting with the viral capsid protein and activating interferon responses., PMID:40340395
Mammalian Queuosine tRNA Modification Impacts Translation to Enhance Cell Proliferation and MHC-II Expression., PMID:40339980
Singapore grouper iridovirus VP018 abrogates the interferon response by targeting STING-TBK1-IRF3 axis., PMID:40339868
The C-terminal nsP1a protein of porcine astrovirus antagonizes interferon beta production by targets MAVS and IRF3 signaling., PMID:40339257
The Brucella Effector Protein BspF Crotonylates TRIM38 to Inhibit NF-κB and MAPK Signaling Pathway., PMID:40332097
Human papillomavirus E1 proteins inhibit RIG-I/MDA5-MAVS, TLR3-TRIF, cGAS-STING, and JAK-STAT signaling pathways to evade innate antiviral immunity., PMID:40330484
Construction and evaluation of a novel Stx2a-based immunotoxin against epidermal growth factor receptor (EGFR)., PMID:40315796
Genome-wide CRISPR screening identifies LRP1 as an entry factor for SFTSV., PMID:40301361
The rabies virus matrix protein (RABV M) interacts with host histone deacetylase 6 (HDAC6) to activate the MEK/ ERK signaling pathway and enhance viral replication., PMID:40300412
Targeting CTP synthetase 1 to restore interferon induction and impede nucleotide synthesis in SARS-CoV-2 infection., PMID:40298378
EV-D68 cleaves LARP1 and PABPC1 by 3Cpro to redirect host mRNA translation machinery toward its genomic RNA., PMID:40294010
Stable expression of SARS-CoV-2 envelope viroporin promotes intracellular calcium depletion in human cells: relevance for endoplasmic reticulum stress, cell proliferation, pluripotency and lineage differentiation., PMID:40286431
Design and Characterization of Inhibitors of Cell-Mediated Degradation of APOBEC3G That Decrease HIV-1 Infectivity., PMID:40284957
Syntaxin-6 restricts SARS-CoV-2 infection by facilitating virus trafficking to autophagosomes., PMID:40277356
HIV-1 Nef activates proviral DNA transcription by recruiting Src kinase to phosphorylate host protein Nef-associated factor 1 to compromise its viral restrictive function., PMID:40272155
The African swine fever virus B125R protein antagonizes JAK-STAT signalling by promoting the degradation of IFNAR2., PMID:40270033
ZBP1 senses splicing aberration through Z-RNA to promote cell death., PMID:40267920
For research use only. Not for human or drug use.
