Anti-AGO2 Antibody (R2P22)

Catalog No.

RHN61801

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

IF: 1:50-1:200, IP: 1:20, WB: 1:500-1:1000

Target

eIF2C 2, AGO2, EIF2C2, Protein argonaute-2, Argonaute RISC catalytic component 2, PPD, eIF-2C 2, Eukaryotic translation initiation factor 2C 2, Protein slicer, PAZ Piwi domain protein, Argonaute2, hAgo2

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9UKV8

Applications

IF, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.

Clone ID

R2P22

Argonaute-2 autoantibodies: a promising biomarker for predicting mortality in HBV-related acute-on-chronic liver failure patients with cirrhosis., PMID:39119295

Nuclear miR-451a activates KDM7A and leads to cetuximab resistance in head and neck squamous cell carcinoma., PMID:38943031

MicroRNA-focused CRISPR/Cas9 screen identifies miR-142 as a key regulator of Epstein-Barr virus reactivation., PMID:38885264

Argonaute, Vault, and Ribosomal Proteins Targeted by Autoantibodies in Systemic Lupus Erythematosus., PMID:37127324

Ago2 and a miRNA reduce Topoisomerase 1 for enhancing DNA cleavage in antibody diversification by activation-induced cytidine deaminase., PMID:37094168

LncRNA MEG3 inhibits renal fibrinoid necrosis of diabetic nephropathy via the MEG3/miR-21/ORAI1 axis., PMID:36715789

Conformation-stabilizing ELISA and cell-based assays reveal patient subgroups targeting three different epitopes of AGO1 antibodies., PMID:36341350

Molecular Pathogenesis of Colorectal Cancer: Impact of Oncogenic Targets Regulated by Tumor Suppressive miR-139-3p., PMID:36232922

lncRNA MAFG‑AS1 enhances radioresistance of glioblastoma cells via miR‑642a‑5p/Notch1 axis., PMID:36214714

IFN-I inducible miR-3614-5p targets ADAR1 isoforms and fine tunes innate immune activation., PMID:35935998

Construction of a gold nanoparticle-based single-molecule biosensor for simple and sensitive detection of Argonaute 2 activity., PMID:35796467

miR-15a and miR-15b modulate natural killer and CD8+T-cell activation and anti-tumor immune response by targeting PD-L1 in neuroblastoma., PMID:35663229

Regulation of microRNA miR-197-3p/CDC28 protein kinase regulatory subunit 1B (CKS1B) axis by Circular RNA hsa_circ_0000285 promotes glioma progression., PMID:35174774

Protein-RNA Interactome Analysis Reveals Wide Association of Kaposi's Sarcoma-Associated Herpesvirus ORF57 with Host Noncoding RNAs and Polysomes., PMID:34787459

MicroRNA-directed pathway discovery elucidates an miR-221/222-mediated regulatory circuit in class switch recombination., PMID:34586363

Argonaute Autoantibodies as Biomarkers in Autoimmune Neurologic Diseases., PMID:34321331

Hsa_circ_0000520 overexpression increases CDK2 expression via miR-1296 to facilitate cervical cancer cell proliferation., PMID:34284793

MicroRNA 195-5p Targets Foxo3 Promoter Region to Regulate Its Expression in Granulosa Cells., PMID:34201585

AGO2 localizes to cytokinetic protrusions in a p38-dependent manner and is needed for accurate cell division., PMID:34117353

Hypoxia-Induced MALAT1 Promotes the Proliferation and Migration of Breast Cancer Cells by Sponging MiR-3064-5p., PMID:34012919

Presence of anti-TIF-1γ, anti-Ro52, anti-SSA/Ro60 and anti-Su/Ago2 antibodies in breast cancer: a cross-sectional study., PMID:33876712

No evidence for Ago2 translocation from the host erythrocyte into the Plasmodium parasite., PMID:33501380

LncRNA AK148321 alleviates neuroinflammation in LPS-stimulated BV2 microglial cell through regulating microRNA-1199-5p/HSPA5 axis., PMID:33301806

LncRNA FAM181A-AS1 promotes gliomagenesis by sponging miR-129-5p and upregulating ZRANB2., PMID:33080570

Extracellular microRNAs in human circulation are associated with miRISC complexes that are accessible to anti-AGO2 antibody and can bind target mimic oligonucleotides., PMID:32929008

The Ago2-miRNA-co-IP Assay to Study TGF- β1 Mediated Recruitment of miRNA to the RISC in CFBE Cells., PMID:32894261

Argonaute binding within 3'-untranslated regions poorly predicts gene repression., PMID:32501500

[The Efficiency of Immunoprecipitation of microRNA/Ago2 Complexes from Human Blood Plasma Is Protocol Dependent]., PMID:32392193

Treatment of breast cancer with autophagy inhibitory microRNAs carried by AGO2-conjugated nanoparticles., PMID:32345308

SNHG9, delivered by adipocyte-derived exosomes, alleviates inflammation and apoptosis of endothelial cells through suppressing TRADD expression., PMID:32007500

Quantum dot nanoconjugates for immuno-detection of circulating cell-free miRNAs., PMID:31816728

Circ_KATNAL1 regulates prostate cancer cell growth and invasiveness through the miR-145-3p/WISP1 pathway., PMID:31800303

Circ_SPECC1 enhances the inhibition of miR-526b on downstream KDM4A/YAP1 pathway to regulate the growth and invasion of gastric cancer cells., PMID:31349968

Dual and Opposing Regulation of MMP1 and MMP13 by Both Arms of miR-675 in Human Articular Chondrocytes., PMID:31264811

LncRNA F11-AS1 suppresses liver hepatocellular carcinoma progression by competitively binding with miR-3146 to regulate PTEN expression., PMID:31168823

RIP-Chip analysis supports different roles for AGO2 and GW182 proteins in recruiting and processing microRNA targets., PMID:30999843

Regulation of cell-type-specific transcriptomes by microRNA networks during human brain development., PMID:30455455

RNA interference may suppress stress granule formation by preventing argonaute 2 recruitment., PMID:30404558

Therapeutic Potential of OMe-PS-miR-29b1 for Treating Liver Fibrosis., PMID:30287074

MicroRNA-223 Regulates Septin-2 and Septin-6 in Stored Platelets., PMID:29943706

Identification and functional analysis of inflammation-related miRNAs in skin wound repair., PMID:29873073

Identifying RISC Components Using Ago2 Immunoprecipitation and Mass Spectrometry., PMID:29236257

Reactivity of human AGO2 monoclonal antibody 11A9 with the SWI/SNF complex: A case study for rigorously defining antibody selectivity., PMID:28779093

Autoantibodies to Su/Argonaute 2 in Japanese patients with inflammatory myopathy., PMID:28673815

Argonaute 2 immunoprecipitation revealed large tumor suppressor kinase 1 as a novel proapoptotic target of miR-21 in T cells., PMID:28075055

Snail-Modulated MicroRNA 493 Forms a Negative Feedback Loop with the Insulin-Like Growth Factor 1 Receptor Pathway and Blocks Tumorigenesis., PMID:27956702

Neuropilin-1 is a receptor for extracellular miRNA and AGO2/miRNA complexes and mediates the internalization of miRNAs that modulate cell function., PMID:27486976

Bio-functional surfaces for the immunocapture of AGO2-bound microRNAs., PMID:27449965

CSN1 Somatic Mutations in Penile Squamous Cell Carcinoma., PMID:27325650

Comprehensive RNA dataset of AGO2 associated RNAs in Jurkat cells following miR-21 over-expression., PMID:27054165

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