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Recombinant Human CD142/F3/TF Protein, C-His

Catalog #:   EHD00601 Specific References (158) DATASHEET
Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Expression system: Mammalian Cells
Accession: P13726
Protein length: Met1-Glu251
Overview

Catalog No.

EHD00601

Description

Tissue factor, also called platelet tissue factor, factor III, or CD142, is a protein encoded by the F3 gene, present in subendothelial tissue and leukocytes. CD142, also known as ,Coagulation Factor III, and Thromboplastin, is a 45 kD type I transmembrane glycoprotein. CD142 is a high-affinity receptor for coagulation factor VII and initiates the extrinsic pathway of blood coagulation. CD142 also plays an important role in a variety of diseases such as sepsis, atherosclerosis, and cancer. It is expressed on the surface of a variety of cells that are physically separated from the circulating blood which include smooth muscle cells, fibroblasts, keratinocytes, glomerular epithelial cells (cytoplasmic inclusions), astrocytes, myocardium, liver stromal cells, pancreas cells, and is also expressed on activated monocytes and stimulated endothelial cells.

Expression system

Mammalian Cells

Species

Homo sapiens (Human)

Protein length

Met1-Glu251

Predicted molecular weight

29.24 kDa

Nature

Recombinant

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Accession

P13726

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

Tissue factor, CD142, F3, Thromboplastin, Coagulation factor III, TF

Note

For research use only.

Data Image
  • SDS-PAGE
    SDS PAGE for recombinant Human CD142 / F3 / TF
  • Bioactivity
    Detects Tisotumab in indirect ELISAs.
References

Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease., PMID:30270042

Elevated coagulation factor levels affect the tissue factor-threshold in thrombin generation., PMID:30408635

Signal Transduction Peptide of Tissue Factor Phosphorylated at Ser258 and the Unphosphorylated STP in Urine Are Potential Biomarkers for Bladder Cancer., PMID:30579699

Intravascular Mesenchymal Stromal/Stem Cell Therapy Product Diversification: Time for New Clinical Guidelines., PMID:30711482

HIV and cardiovascular diseases risk: exploring the interplay between T-cell activation, coagulation, monocyte subsets, and lipid subclass alterations., PMID:30768357

Adult human pancreatic acinar cells dedifferentiate into an embryonic progenitor-like state in 3D suspension culture., PMID:30858455

Identification of a mesenchymal progenitor cell hierarchy in adipose tissue., PMID:31023895

Annexin V+ Microvesicles in Children and Adolescents with Type 1 Diabetes: A Prospective Cohort Study., PMID:32309448

CD142 plays an important role in the mobility of colorectal cancer cells., PMID:32471327

Frail older adults show a distinct plasma microvesicle profile suggesting a prothrombotic and proinflammatory phenotype., PMID:32749745

Predictive Markers for Malignant Urothelial Transformation in Balkan Endemic Nephropathy: A Case-Control Study., PMID:33065960

Current Targets and Bioconjugation Strategies in Photodynamic Diagnosis and Therapy of Cancer., PMID:33121022

[Level of Microparticles in Patients with Essential Thrombocythemia and Its Relation with JAK2V617F Mutation]., PMID:33283740

PAR-4/Ca2+-calpain pathway activation stimulates platelet-derived microparticles in hyperglycemic type 2 diabetes., PMID:33812377

Plasma levels of extracellular vesicles and the risk of post-operative pulmonary embolism in patients with primary brain tumors: a prospective study., PMID:33837918

Circulating extracellular vesicles are endowed with enhanced procoagulant activity in SARS-CoV-2 infection., PMID:33971404

Expansion of GARP-Expressing CD4+CD25-FoxP3+ T Cells and SATB1 Association with Activation and Coagulation in Immune Compromised HIV-1-Infected Individuals in South Africa., PMID:33974229

Critical COVID-19 is associated with distinct leukocyte phenotypes and transcriptome patterns., PMID:34080738

CD142 plays a key role in the carcinogenesis of gastric adenocarcinoma by inhibiting BCL2-dependent autophagy., PMID:34289309

Increased Expression of Extracellular Vesicles Is Associated With the Procoagulant State in Patients With Established Rheumatoid Arthritis., PMID:34394126

Mesenchymal stem cell transfusion: Possible beneficial effects in COVID-19 patients., PMID:34419356

Platelet-derived extracellular vesicles may contribute to the hypercoagulable state in preeclampsia., PMID:34534879

Identification of a regulatory pathway inhibiting adipogenesis via RSPO2., PMID:35027768

Pregnancy tailors endotoxin-induced monocyte and neutrophil responses in the maternal circulation., PMID:35445873

Risk stratification of patients with SARS-CoV-2 by tissue factor expression in circulating extracellular vesicles., PMID:35597450

Improved MSC Minimal Criteria to Maximize Patient Safety: A Call to Embrace Tissue Factor and Hemocompatibility Assessment of MSC Products., PMID:35641163

Mammalian adipogenesis regulator (Areg) cells use retinoic acid signalling to be non- and anti-adipogenic in age-dependent manner., PMID:35996853

Citicoline Modifies the Expression of Specific miRNAs Related to Cardioprotection in Patients with ST-Segment Elevation Myocardial Infarction Subjected to Coronary Angioplasty., PMID:36015073

Advanced cell therapy with low tissue factor loaded product NestaCell® does not confer thrombogenic risk for critically ill COVID-19 heparin-treated patients., PMID:36068779

Streptococcal arginine deiminase regulates endothelial inflammation, mTOR pathway and autophagy., PMID:36746072

CD142 promotes trophoblast cell migration by inhibiting BCL2-related autophagic degradation of IL-8., PMID:36847889

Characterization of CRISPR/Cas9-edited human placental allogenic stromal cells with low tissue factor expression and reduced thrombotic effects., PMID:37256239

Evaluation of four commercial ELISAs to measure tissue factor in human plasma., PMID:37275179

CD142 Identifies Neoplastic Desmoid Tumor Cells, Uncovering Interactions Between Neoplastic and Stromal Cells That Drive Proliferation., PMID:37377751

Tissue factor is a critical regulator of radiation therapy-induced glioblastoma remodeling., PMID:37451272

High-Dimensional Single-Cell Multimodal Landscape of Human Carotid Atherosclerosis., PMID:37502836

High CD142 Level Marks Tumor-Promoting Fibroblasts with Targeting Potential in Colorectal Cancer., PMID:37511344

Heterotopia of salivary gland tissue in the pancreas., PMID:37649044

Two-step regulation by matrix Gla protein in brown adipose cell differentiation., PMID:38184275

Extracellular vesicles are a late marker of inflammation, hypercoagulability and COVID-19 severity., PMID:38341321

Targeting cancer-derived extracellular vesicles by combining CD147 inhibition with tissue factor pathway inhibitor for the management of urothelial cancer cells., PMID:38360687

High-Dimensional Single-Cell Multimodal Landscape of Human Carotid Atherosclerosis., PMID:38385291

Intestinal stroma guides monocyte differentiation to macrophages through GM-CSF., PMID:38409190

Impact of Early Microparticle Release during Isolated Severe Traumatic Brain Injury: Correlation with Coagulopathy and Mortality., PMID:38691471

Conversion of anti-tissue factor antibody sequences to chimeric antigen receptor and bi-specific T-cell engager format., PMID:39105809

A loss of tuning of both pro-coagulant and inflammatory responses in monocytes in patients with preeclampsia., PMID:39332076

Analysis of small extracellular vesicles from dried blood spots., PMID:39931421

Multi-omics analysis of uterine fluid extracellular vesicles reveals a resemblance with endometrial tissue across the menstrual cycle: biological and translational insights., PMID:40084293

Aregs-IGFBP3-mediated SMC-like cells apoptosis impairs beige adipocytes formation in aged mice., PMID:40118146

Association between extracellular vesicles (EVs) and thrombosis in antiphospholipid syndrome., PMID:40123163

Detection of tissue factor-positive microparticles and its clinical significance in the haemostatic disorder. PMID: 22931659

Platelet-derived microparticles and coagulation activation in breast cancer patients. PMID: 18841290

Difference in the blood monocyte phenotype between uremic patients and healthy controls: its relation to monocyte differentiation into macrophages in the peritoneal cavity. PMID: 9484650

The detection of ADAM8 protein on cells of the human immune system and the demonstration of its expression on peripheral blood B cells, dendritic cells and monocyte subsets. PMID: 17270707

Immunologic phenotype of cultured endothelial cells: quantitative analysis of cell surface molecules. PMID: 9389318

Immunophenotypic characterisation of carotid plaque: increased amount of inflammatory cells as an independent predictor for ischaemic symptoms. PMID: 11397022

Atorvastatin reduces thrombin generation and expression of tissue factor, P-selectin and GPIIIa on platelet-derived microparticles in patients with peripheral arterial occlusive disease. PMID: 21614411

Circulating microparticle signature in coronary and peripheral blood of ST elevation myocardial infarction patients in relation to pain-to-PCI elapsed time. PMID: 26432487

Distribution of alpha thalassaemia gene variants in diverse ethnic populations in malaysia: data from the institute for medical research. PMID: 24025420

Separating the roles of nitrogen and oxygen in high pressure-induced blood-borne microparticle elevations, neutrophil activation, and vascular injury in mice. PMID: 26048974

A Simple, Rapid, and Highly Sensitive Electrochemical DNA Sensor for the Detection of α- and β-Thalassemia in China. PMID: 27228385

Adenosine inhibition of adenosine diphosphate and thrombin-induced monocyte-platelet aggregates in cardiac syndrome X. PMID: 19185336

Intracoronary platelet and monocyte activation status within platelet-monocyte complexes are determinants of inflammation in ST elevation myocardial infarction1. PMID: 26890234

Diabetes worsening of hepatitis C cirrhosis: are alterations in monocytic tissue factor (CD 142) is the cause? PMID: 24378263

The impact of various preanalytical treatments on the phenotype of small extracellular vesicles in blood analyzed by protein microarray. PMID: 27568281

Oral glucocorticoid treatment decreases interleukin-10 receptor expression on peripheral blood leucocyte subsets. PMID: 19292765

Vitamin D3 down-regulates monocyte TLR expression and triggers hyporesponsiveness to pathogen-associated molecular patterns. PMID: 16402404

Number of microparticles generated during acute myocardial infarction and stable angina correlates with platelet activation. PMID: 22306248

A monoclonal antibody against hinge-cleaved IgG restores effector function to proteolytically-inactivated IgGs in vitro and in vivo. PMID: 25517311

First report of the spectrum of δ-globin gene mutations in Omani subjects - identification of novel mutations. PMID: 25043855

Activated Platelet-Derived and Leukocyte-Derived Circulating Microparticles and the Risk of Thrombosis in Heparin-Induced Thrombocytopenia: A Role for PF4-Bearing Microparticles? PMID: 28052584

Correlative analysis of nanoparticle tracking, flow cytometric and functional measurements for circulating microvesicles in normal subjects. PMID: 27429418

Hb A2 Episkopi - a novel δ-globin chain variant [HBD:c.428C>T] in a family of mixed Cypriot-Lebanese descent. PMID: 28007020

Expansion of GARP-Expressing CD4+CD25-FoxP3+ T Cells and SATB1 Association with Activation and Coagulation in Immune Compromised HIV-1-Infected Individuals in South Africa. PMID: 33974229

A subpopulation of human B lymphocytes can express a functional Tissue Factor in response to phorbol myristate acetate. PMID: 16113798

Levels of activated platelet-derived microvesicles in patients with soft tissue sarcoma correlate with an increased risk of venous thromboembolism. PMID: 28784104

Circulating microparticles, protein C, free protein S and endothelial vascular markers in children with sickle cell anaemia. PMID: 26609806

Elevated procoagulant endothelial and tissue factor expressing microparticles in women with recurrent pregnancy loss. PMID: 24278436

Endothelial cell activation and neovascularization are prominent in dermatomyositis. PMID: 16504012

MEIS1 and MEIS2 Expression and Prostate Cancer Progression: A Role For HOXB13 Binding Partners in Metastatic Disease. PMID: 29716922

Evaluation of Alpha-Thalassemia Mutations in Cases with Hypochromic Microcytic Anemia: The İstanbul Perspective. PMID: 26377141

Extracellular Vesicle Surface Markers as a Diagnostic Tool in Transient Ischemic Attacks. PMID: 34344167

Flow cytometric analysis of microparticle phenotype and their role in thrombin generation. PMID: 20632415

Atraumatic Pulsatile Leukocyte Circulation for Long-Term In Vitro Dynamic Culture and Adhesion Assays. PMID: 25894522

Ascorbic acid supplementation diminishes microparticle elevations and neutrophil activation following SCUBA diving. PMID: 26084697

Cancer Cell-Derived Extracellular Vesicles Are Associated with Coagulopathy Causing Ischemic Stroke via Tissue Factor-Independent Way: The OASIS-CANCER Study. PMID: 27427978

Platelet leukocyte aggregates and markers of platelet aggregation, immune activation and disease progression in HIV infected treatment naive asymptomatic individuals. PMID: 25899563

Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells. PMID: 21804561

PARP-14 combines with tristetraprolin in the selective posttranscriptional control of macrophage tissue factor expression. PMID: 25293769

Elevated levels of circulating microvesicles in coronary artery disease patients with type 2 diabetes and albuminuria: Effects of exercise training. PMID: 31023084

Tumor-associated macrophages promote invasion while retaining Fc-dependent anti-tumor function. PMID: 23105143

Phenotypic mapping of human mesothelial cells. PMID: 10649685

Metformin reduces total microparticles and microparticles-expressing tissue factor in women with polycystic ovary syndrome. PMID: 28795250

Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells. PMID: 29138638

Ascorbic acid abrogates microparticle generation and vascular injuries associated with high-pressure exposure. PMID: 25977448

Platelet, monocyte and neutrophil activation and glucose tolerance in South African Mixed Ancestry individuals. PMID: 28091589

Enumeration and phenotyping of circulating microvesicles by flow cytometry and nanoparticle tracking analysis: Plasma versus serum. PMID: 33244869

Selection of Tissue Factor-Deficient Cell Transplants as a Novel Strategy for Improving Hemocompatibility of Human Bone Marrow Stromal Cells. PMID: 29507631

Profiling of the three circulating monocyte subpopulations in human obesity. PMID: 25786686

Circulating levels and characterization of microparticles in patients with different degrees of glucose tolerance. PMID: 28927403

Markers of endothelial damage in patients with chronic kidney disease on hemodialysis. PMID: 28077371

Signal Transduction Peptide of Tissue Factor Phosphorylated at Ser258 and the Unphosphorylated STP in Urine Are Potential Biomarkers for Bladder Cancer. PMID: 30579699

Soluble endothelial cell molecules and circulating endothelial cells in patients with venous thromboembolism. PMID: 28661913

HIV and cardiovascular diseases risk: exploring the interplay between T-cell activation, coagulation, monocyte subsets, and lipid subclass alterations. PMID: 30768357

Temporal dynamics of microparticle elevation following subarachnoid hemorrhage. PMID: 22794324

Elevated coagulation factor levels affect the tissue factor-threshold in thrombin generation. PMID: 30408635

Studies of microparticles in patients with the antiphospholipid syndrome (APS). PMID: 22635239

Adverse effects of fullerenes on endothelial cells: fullerenol C60(OH)24 induced tissue factor and ICAM-I membrane expression and apoptosis in vitro. PMID: 18488416

Endothelial Microparticles and Blood Coagulation Activation in Head and Neck Cancer Patients Undergoing Radiotherapy or Radiochemotherapy. PMID: 30804151

Mesenchymal stromal cell therapy for coronavirus disease 2019: which? when? and how much? PMID: 34053857

Association of microparticles and neutrophil activation with decompression sickness. PMID: 26139218

Mesenchymal stem cell transfusion: Possible beneficial effects in COVID-19 patients. PMID: 34419356

Reconstructing human pancreatic differentiation by mapping specific cell populations during development. PMID: 28731406

Activated platelets and leukocyte activations in young patients with β-thalassemia/HbE following bone marrow transplantation. PMID: 29990620

Anti-domain 1 β2 glycoprotein antibodies increase expression of tissue factor on monocytes and activate NK Cells and CD8+ cells in vitro. PMID: 32127041

PAR-4/Ca2+-calpain pathway activation stimulates platelet-derived microparticles in hyperglycemic type 2 diabetes. PMID: 33812377

Flow cytometric characterization of the saphenous veins endothelial cells in patients with chronic venous disease and in patients undergoing bypass surgery: an exploratory study. PMID: 31227876

Procoagulant activity of human mesenchymal stem cells. PMID: 28628602

Plasma levels of extracellular vesicles and the risk of post-operative pulmonary embolism in patients with primary brain tumors: a prospective study. PMID: 33837918

Elevated circulating levels of tissue factor-positive microvesicles are associated with distant metastasis in lung cancer. PMID: 24169761

Seminal clotting and fibrinolytic balance: a possible physiological role in the male reproductive system. PMID: 15467906

Increased Expression of Extracellular Vesicles Is Associated With the Procoagulant State in Patients With Established Rheumatoid Arthritis. PMID: 34394126

Circulating endothelial cells in patients with venous thromboembolism and myeloproliferative neoplasms. PMID: 24339944

A multicolor flow cytometric assay for measurement of platelet-derived microparticles. PMID: 19939440

Non-neoplastic Portal Vein Thrombosis in HCV Cirrhosis Patients: Is it an Immuno-Inflammatory Disorder? PMID: 28611261

17β-Estradiol Promotes Proinflammatory and Procoagulatory Phenotype of Innate Immune Cells in the Presence of Antiphospholipid Antibodies. PMID: 32549383

The biology and tumour-related properties of monocyte tissue factor. PMID: 11169509

Possibility of Coagulation System Activation Determination with Tissue Factor in Pregnancy Complications. PMID: 28164533

Annexin V+ Microvesicles in Children and Adolescents with Type 1 Diabetes: A Prospective Cohort Study. PMID: 32309448

The Mediterranean diet decreases prothrombotic microvesicle release in asymptomatic individuals at high cardiovascular risk. PMID: 32147198

Critical COVID-19 is associated with distinct leukocyte phenotypes and transcriptome patterns. PMID: 34080738

Tissue factor: a key molecule in hemostatic and nonhemostatic systems. PMID: 15005335

Monocyte tissue factor (CD142) and Mac-1 (CD11b) are increased in septic patients. PMID: 9657455

Platelet-derived microparticles during and after acute coronary syndrome. PMID: 22371053

Adult human pancreatic acinar cells dedifferentiate into an embryonic progenitor-like state in 3D suspension culture. PMID: 30858455

Effects of acute insulin-induced hypoglycaemia on endothelial microparticles in adults with and without type 2 diabetes. PMID: 30264480

Platelet-, monocyte-derived and tissue factor-carrying circulating microparticles are related to acute myocardial infarction severity. PMID: 28207887

Predictive Markers for Malignant Urothelial Transformation in Balkan Endemic Nephropathy: A Case-Control Study. PMID: 33065960

[Level of Microparticles in Patients with Essential Thrombocythemia and Its Relation with JAK2V617F Mutation]. PMID: 33283740

Circulating extracellular vesicles are endowed with enhanced procoagulant activity in SARS-CoV-2 infection. PMID: 33971404

Current Targets and Bioconjugation Strategies in Photodynamic Diagnosis and Therapy of Cancer. PMID: 33121022

Tissue factor in the antiphospholipid syndrome. PMID: 18827061

Frail older adults show a distinct plasma microvesicle profile suggesting a prothrombotic and proinflammatory phenotype. PMID: 32749745

Interstitial Cell Remodeling Promotes Aberrant Adipogenesis in Dystrophic Muscles. PMID: 32375047

Increase in expression of monocytic tissue factor (CD142) with monocytes and blood platelet activation in liver cirrhosis. PMID: 17982314

64Cu-1,4,7-Triazacyclononane-1,4,7-triacetic acid-p-isothiocyanatobenzyl-ALT-836. PMID: 23534079

Monocyte-derived circulating microparticles (CD14(+), CD14(+)/CD11b(+) and CD14(+)/CD142(+)) are related to long-term prognosis for cardiovascular mortality in STEMI patients. PMID: 27915085

Putative Natural History of CoViD-19. PMID: 32831521

High levels of TSP1+/CD142+ platelet-derived microparticles characterise young patients with high cardiovascular risk and subclinical atherosclerosis. PMID: 26178021

Intravascular Mesenchymal Stromal/Stem Cell Therapy Product Diversification: Time for New Clinical Guidelines. PMID: 30711482

MSC Therapies for COVID-19: Importance of Patient Coagulopathy, Thromboprophylaxis, Cell Product Quality and Mode of Delivery for Treatment Safety and Efficacy. PMID: 32574263

Single-cell RNA-seq highlights heterogeneity in human primary Wharton's jelly mesenchymal stem/stromal cells cultured in vitro. PMID: 32252818

CD142+/CD61+, CD146+ and CD45+ microparticles predict cardiovascular events in high risk patients following a Mediterranean diet supplemented with nuts. PMID: 27052787

A stromal cell population that inhibits adipogenesis in mammalian fat depots. PMID: 29925944

CD142 plays a key role in the carcinogenesis of gastric adenocarcinoma by inhibiting BCL2-dependent autophagy. PMID: 34289309

Identification of a mesenchymal progenitor cell hierarchy in adipose tissue. PMID: 31023895

Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease. PMID: 30270042

CD142 plays an important role in the mobility of colorectal cancer cells. PMID: 32471327

Datasheet
$ 480
Product specifications
100 μg 480 1 mg 2880

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Recombinant Human CD142/F3/TF Protein, C-His [EHD00601]
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