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Anti-Phospho-SMAD1 (Ser463/Ser465) Antibody (R2G70)

Catalog #:   RHH20101 Specific References (18) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: IP, WB
Accession: Q15797
Overview

Catalog No.

RHH20101

Species reactivity

Human, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

IP: 1:20, WB: 1:500-1:1000

Target

BSP1, Mothers against DPP homolog 1, Mothers against decapentaplegic homolog 1, SMAD1, Transforming growth factor-beta-signaling protein 1, MADH1, JV4-1, MADR1, SMAD family member 1, hSMAD1, SMAD 1, MAD homolog 1, Mad-related protein 1, BSP-1, Smad1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q15797

Applications

IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.

Clone ID

R2G70

Data Image
  • Western blot
    Western blot analysis of Phospho-Smad1 (Ser463/Ser465) in A549, U2OS, C6 lysates using Phospho-Smad1 (Ser463/Ser465) antibody.
References

Specific Blockade of Bone Morphogenetic Protein-2/4 Induces Oligodendrogenesis and Remyelination in Demyelinating Disorders., PMID:34159538

Immunofluorescent Visualization of BMP Signaling Activation on Paraffin-Embedded Tissue Sections., PMID:30414134

Organizer-derived Bmp2 is required for the formation of a correct Bmp activity gradient during embryonic development., PMID:24777107

Low-intensity pulsed ultrasound induces osteogenic differentiation of human periodontal ligament cells through activation of bone morphogenetic protein-smad signaling., PMID:24764342

The immunoglobulin superfamily protein differentiation of embryonic stem cells 1 (dies1) has a regulatory role in preadipocyte to adipocyte conversion., PMID:23799023

Hic-5 controls BMP4 responses in prostate cancer cells through interacting with Smads 1, 5 and 8., PMID:21996749

Alk3 controls nephron number and androgen production via lineage-specific effects in intermediate mesoderm., PMID:21613322

Activation of Src mediates PDGF-induced Smad1 phosphorylation and contributes to the progression of glomerulosclerosis in glomerulonephritis., PMID:21445358

Detection of signaling effector-complexes downstream of bmp4 using PLA, a proximity ligation assay., PMID:21403637

A sensitive detection of phospho-Smad1/5/8 and Smad2 in Western blot analyses., PMID:17976733

Functional role of bone morphogenetic protein-4 in isolated canine parietal cells., PMID:17600042

Cyclooxygenase-2 expression in polyps from a patient with juvenile polyposis syndrome with mutant BMPR1A., PMID:17325551

The synthetic triterpenoid CDDO-imidazolide induces monocytic differentiation by activating the Smad and ERK signaling pathways in HL60 leukemia cells., PMID:16818503

A novel vitamin D derivative activates bone morphogenetic protein signaling in MCF10 breast epithelial cells., PMID:16533909

Activation of STAT3/Smad1 is a key signaling pathway for progression to glomerulosclerosis in experimental glomerulonephritis., PMID:15591053

BMP and activin receptor expression in lens development., PMID:15346106

Initiation of Smad-dependent and Smad-independent signaling via distinct BMP-receptor complexes., PMID:12925609

Endogenous patterns of TGFbeta superfamily signaling during early Xenopus development., PMID:10851136

Datasheet
$ 290
Product specifications
50 μg 290 100 μg 484

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Phospho-SMAD1 (Ser463/Ser465) Antibody (R2G70) [RHH20101]
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