Catalog No.
RHG68301
Species reactivity
Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
WB: 1:500-1:1000
Target
Ras-specific guanine nucleotide-releasing factor 1, Ras-GRF1, GNRP, RASGRF1, CDC25, Guanine nucleotide-releasing protein, Ras-specific nucleotide exchange factor CDC25, GRF1
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q13972
Applications
WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R2F12
Rare molecular subtypes of lung cancer., PMID:36806787
Arp2/3-Branched Actin Maintains an Active Pool of GTP-RhoA and Controls RhoA Abundance., PMID:31623230
Modulation of B-cell receptor and microenvironment signaling by a guanine exchange factor in B-cell malignancies., PMID:27458535
Amplification of B cell receptor-Erk signaling by Rasgrf-1 overexpression in chronic lymphocytic leukemia., PMID:24597981
Rgnef (p190RhoGEF) knockout inhibits RhoA activity, focal adhesion establishment, and cell motility downstream of integrins., PMID:22649559
The Ras guanine nucleotide exchange factor RasGRF1 promotes matrix metalloproteinase-3 production in rheumatoid arthritis synovial tissue., PMID:19678938
The budding yeast RasGEF Cdc25 reveals an unexpected nuclear localization., PMID:18930081
Human Raf-1 proteins associate with Rad24 and Cdc25 in cell-cycle checkpoint pathway of fission yeast, Schizosaccharomyces pombe., PMID:17243098
Haloperidol induces neurotoxicity by the NMDA receptor downstream signaling pathway, alternative from glutamate excitotoxicity., PMID:17092607
Physical and functional interactions between polo kinase and the spindle pole component Cut12 regulate mitotic commitment in S. pombe., PMID:12815070
Phosphorylation of the Ras-GRF1 exchange factor at Ser916/898 reveals activation of Ras signaling in the cerebral cortex., PMID:12538592
Regulation of p70 S6 kinase by complex formation between the Rac guanine nucleotide exchange factor (Rac-GEF) Tiam1 and the scaffold spinophilin., PMID:12531897
Characterization of membrane-localized and cytosolic Rac-GTPase-activating proteins in human neutrophil granulocytes: contribution to the regulation of NADPH oxidase., PMID:11311150
A 54-kDa protein related to ras-guanine nucleotide release factor expressed in the rat exocrine pancreas., PMID:9232829
The production of soluble interleukin 4 receptors is preferentially regulated by the murine Th2 cell subset., PMID:9126705
Expression of alternative forms of Ras exchange factors GRF and SOS1 in different human tissues and cell lines., PMID:8649802
Membrane-anchoring domains of Cdc25p, a Saccharomyces cerevisiae ras exchange factor., PMID:8893498
The cellular content of Cdc25p, the Ras exchange factor in Saccharomyces cerevisiae, is regulated by destabilization through a cyclin destruction box., PMID:7657656
Changes in tyrosine-phosphorylated p190 and its association with p120 type I and p100 type II rasGAPs during myelomonocytic differentiation of human leukemic cells., PMID:7756172
Cloning and analysis of human cDNAs encoding a 140-kDa brain guanine nucleotide-exchange factor, Cdc25GEF, which regulates the function of Ras., PMID:7828890
Epidermal growth factor induces protein tyrosine phosphorylation and association of p190 with ras-GTP-ase activating protein in Caco-2 cells., PMID:7926013
Expression of two different products of CDC25Mm, a mammalian Ras activator, during development of mouse brain., PMID:8299730
Identification of residues of the H-ras protein critical for functional interaction with guanine nucleotide exchange factors., PMID:8289791
Identification of a human guanine nucleotide-releasing factor (H-GRF55) specific for Ras proteins., PMID:7684828
The C-terminal part of the CDC25 gene product has Ras-nucleotide exchange activity when present in a chimeric SDC25-CDC25 protein., PMID:8319295
A Candida albicans homolog of CDC25 is functional in Saccharomyces cerevisiae., PMID:8477693
A murine CDC25/ras-GRF-related protein implicated in Ras regulation., PMID:8293576
Phosphorylation of the S. cerevisiae Cdc25 in response to glucose results in its dissociation from Ras., PMID:1334534
Isolation of multiple mouse cDNAs with coding homology to Saccharomyces cerevisiae CDC25: identification of a region related to Bcr, Vav, Dbl and CDC24., PMID:1396590
Molecular cloning of cDNAs encoding a guanine-nucleotide-releasing factor for Ras p21., PMID:1379346
Anti-Cdc25 antibodies inhibit guanyl nucleotide-dependent adenylyl cyclase of Saccharomyces cerevisiae and cross-react with a 150-kilodalton mammalian protein., PMID:1588963
p55CDC25 is a nuclear protein required for the initiation of mitosis in human cells., PMID:1961714
Molecular cloning and characterization of a factor that binds the human glucocorticoid receptor gene and represses its expression., PMID:1894621
Purification of a human glucocorticoid receptor gene promoter-binding protein. Production of polyclonal antibodies against the purified factor., PMID:2026589
Overexpression of the CDC25 gene, an upstream element of the RAS/adenylyl cyclase pathway in Saccharomyces cerevisiae, allows immunological identification and characterization of its gene product., PMID:2121145
Intramitotic controls in the fission yeast Schizosaccharomyces pombe: the effect of cell size on spindle length and the timing of mitotic events., PMID:2186047
For research use only. Not for human or drug use.
