Catalog No.
RHF62734
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Applications
IF, IHC, ChIP, WB
Recommended Dilution
| Application |
Dilution |
| IF |
1:50-1:200, IHC: 1:100-1:200, ChIP: 1:20-1:50, WB: 1:1000-1:2000 |
Target
Histone H3/j, Histone H3/b, Histone H3/d, Histone H3/c, HIST1H3B, H3FH, Histone H3/f, H3FF, H3FK, HIST1H3G, H3FL, H3FB, HIST1H3D, Histone H3/a, H3FI, Histone H3/i, H3FD, HIST1H3A, Histone H3/h, HIST1H3I, HIST1H3E, HIST1H3H, HIST1H3J, H3C1, H3FC HIST1H3C, H3FA, Histone H3.1, H3FJ, Histone H3/l, Histone H3/k, HIST1H3F
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P68431, P84243, Q16695, Q6NXT2, Q71DI3
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R2B08
Note
For research use only.
Monoallelic expression can govern penetrance of inborn errors of immunity., PMID:39743591
Quantification of Citrullinated Histone H3 as a Marker for Neutrophil Extracellular Traps Correlated to Clinical Characteristics of Patients with Systemic Lupus Erythematosus., PMID:39752730
By activating endothelium histone H4 mediates oleic acid-induced acute respiratory distress syndrome., PMID:39757148
Quantitative Analysis of Nonhistone Lysine Methylation Sites and Lysine Demethylases in Breast Cancer Cell Lines., PMID:39778878
Short-chain fatty acid metabolites propionate and butyrate are unique epigenetic regulatory elements linking diet, metabolism and gene expression., PMID:39789354
Binding mode-guided development of high-performance antibodies targeting site-specific posttranslational modifications., PMID:39793060
Chemical proteomic profiling of lysine crotonylation using minimalist bioorthogonal probes in mammalian cells., PMID:39817196
Distinct structural and functional heterochromatin partitioning of lamin B1 and lamin B2 revealed using genome-wide nicking enzyme epitope targeted DNA sequencing., PMID:39817518
T-2 toxin triggers immunotoxic effects in goats by inducing ferroptosis and neutrophil extracellular traps., PMID:39832565
Mineral Stress Drives Loss of Heterochromatin: An Early Harbinger of Vascular Inflammaging and Calcification., PMID:39840455
Protocol for visualizing the chromatin assembly properties of epigenetic protein complexes via an HTM module-mediated artificial tethering system., PMID:39879359
Low-fecundity dhc-1; mel-28 C. elegans mutants do not have gonad mitosis defects., PMID:39911910
SDG8 and HUB2 depositing euchromatin histone marks play important roles in meiosis and crossing-over regulation., PMID:39916632
Influence of sodium ferulate on neutrophil extracellular traps-platelet activation-mediated endothelial dysfunction in immune small vasculitis., PMID:39917008
The added value of coupling anti-dsDNA and anti-chromatin antibodies in follow-up monitoring of systemic lupus erythematosus patients., PMID:39917317
Anaerobic metabolism promotes breast cancer survival via Histone-3 Lysine-18 lactylation mediating PPARD axis., PMID:39922804
Blocking peptidyl arginine deiminase 4 confers neuroprotective effect in the post-ischemic brain through both NETosis-dependent and -independent mechanisms., PMID:39966968
The effect of chemical fixation with paraformaldehyde, glutardialdehyde or methanol on immunofluorescence staining of neutrophils and neutrophil extracellular traps., PMID:39967319
N-terminal modification of Histone H3 inhibits H3K27M-mediated loss of H3K27 trimethylation., PMID:39974979
Histone H3 K4 trimethylation occurs mainly at the origins of polycistronic transcription in the genome of Leishmania infantum promastigotes and intracellular amastigotes., PMID:39979847
Effects of human immunoglobulin A on Cryptococcus neoformans morphology and gene expression., PMID:39982066
Hepatocytes and Hepatic Stellate Cells Carry Different Levels of DNA Damage due to Their Sensitivity to Oxidative Stress in Chronic Hepatitis B., PMID:39991880
Inhibition of EETosis with an anti-citrullinated histone antibody: a novel therapeutic approach for eosinophilic inflammatory disorders., PMID:40051617
Appraising histone H4 lysine 5 lactylation as a novel biomarker in breast cancer., PMID:40065036
Deficiency of H3K27 histone demethylase UTX in T cells blunts allergic sensitization and anaphylaxis to peanut., PMID:40065718
Targeted DamID detects cell-type-specific histone modifications in intact tissues or organisms., PMID:40067796
EZH2 coordinates memory B-cell programming and recall responses., PMID:40073167
Role of H3F3A (H3G34W) antibody in the diagnosis of giant cell tumour of bone in the paediatric age group., PMID:40107010
PI3Kβ functions as a protein kinase to promote cellular protein O-GlcNAcylation and acetyl-CoA production for tumor growth., PMID:40132583
Mind Your Spectra: Points to be Aware of When Validating the Identification of Isobaric Histone Peptidoforms., PMID:40163023
Immune Response and Production of Abzymes in Patients with Autoimmune and Neurodegenerative Diseases., PMID:40164167
Nuclear Galectin-1 promotes KRAS-dependent activation of pancreatic cancer stellate cells., PMID:40172967
Platelet activating histone/antihistone IgG complexes in anti-PF4 negative thrombosis and thrombocytopenia syndrome., PMID:40179418
Multiplexed cytometry for single cell chemical biology., PMID:40180452
The mechanism of EZH2/H3K27me3 downregulating CXCL10 to affect CD8+ T cell exhaustion to participate in the transformation from myelodysplastic syndrome to acute myeloid leukaemia., PMID:40201935
Valproic acid promotes transcriptional activation of Drd2 by mediating histone acetylation to inhibit the mTOR-Pttg1 signaling axis and exerts anti-PitNETs activity., PMID:40220407
SIRT3 Functions as an Eraser of Histone H3K9 Lactylation to Modulate Transcription for Inhibiting the Progression of Esophageal Cancer., PMID:40252727
Detection of γ-H2A.X for Rapid Assessment of Genotoxic Agent-induced Double-strand DNA Breaks by Immunofluorescence., PMID:40257558
Neutrophil extracellular traps aggravate placental injury in OAPS by facilitating activation of BNIP3 mediated mitophagy., PMID:40286883
Two CENH3 paralogs in the green alga Chlamydomonas reinhardtii have a redundantly essential function and associate with ZeppL-LINE1 elements., PMID:40289909
Surgical and radiological outcomes of giant cell tumor of the bone: prognostic value of Campanacci grading and selective use of denosumab., PMID:40317378
ATAD2 bromodomain in cancer therapy: current status and future perspectives., PMID:40334884
Evaluation of plasma nucleosome concentrations and the effect of pre-analytical variables in healthy cats., PMID:40382584
Synergistic inhibition of CitH3 and S100A8/A9: A novel therapeutic strategy for mitigating sepsis-induced inflammation and lung injury., PMID:40390290
Epigenomic Profiling of Histone Posttranslational Modifications in Frozen Cancer Tissue Using CUT&RUN., PMID:40402458
Anti-DAMP therapies for acute inflammation., PMID:40406124
Radiation-Induced Gamma-H2AX Foci Staining and Analysis., PMID:40418470
Mechanistic insights into the stimulation of the histone H3K9 methyltransferase Clr4 by proximal H3K14 ubiquitination., PMID:40446033
KMT2D coordinates antiviral CD4+ T cell responses through opposing effects on T follicular helper and cytotoxic gene expression., PMID:40460200
Immune-modulatory effects of Spindlin-1 inhibitors., PMID:40512144
For research use only. Not for human or drug use.
