Catalog No.
RHE14201
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IF: 1:50-1:200, IHC: 1:100-1:200, WB: 1:1000-1:2000
Target
ADRBK2, Beta-adrenergic receptor kinase 2, BARK2, Beta-ARK-2, GRK3, G-protein-coupled receptor kinase 3
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P35626
Applications
IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1V20
Suitability of GRK Antibodies for Individual Detection and Quantification of GRK Isoforms in Western Blots., PMID:35163118
Agonist-selective NOP receptor phosphorylation correlates in vitro and in vivo and reveals differential post-activation signaling by chemically diverse agonists., PMID:30914485
Hierarchical organization of multi-site phosphorylation at the CXCR4 C terminus., PMID:23734232
Tissue dependent differences in G-protein coupled receptor kinases associated with 5-HT4 receptor desensitization in the rat gastro-intestinal tract., PMID:20836992
Pasireotide and octreotide stimulate distinct patterns of sst2A somatostatin receptor phosphorylation., PMID:20051480
Site-specific phosphorylation of CXCR4 is dynamically regulated by multiple kinases and results in differential modulation of CXCR4 signaling., PMID:20048153
Tyrosine phosphorylation of Kir3 following kappa-opioid receptor activation of p38 MAPK causes heterologous desensitization., PMID:19773548
Extracellular signal-regulated kinase 1/2-mediated transcriptional regulation of G-protein-coupled receptor kinase 3 expression in neuronal cells., PMID:17255468
Decreased expression of G protein-coupled receptor kinases in the detrusor smooth muscle of human urinary bladder with outlet obstruction., PMID:16984558
The fine-structural distribution of G-protein receptor kinase 3, beta-arrestin-2, Ca2+/calmodulin-dependent protein kinase II and phosphodiesterase PDE1C2, and a Cl(-)-cotransporter in rodent olfactory epithelia., PMID:16374707
Differential phosphorylation and dephosphorylation of beta2-adrenoceptor sites Ser262 and Ser355,356., PMID:16331289
Myocardial expression and redistribution of GRKs in hypertensive hypertrophy and failure., PMID:15584034
Raf-1 kinase inhibitor protein: structure, function, regulation of cell signaling, and pivotal role in apoptosis., PMID:15327891
Neuropathic pain activates the endogenous kappa opioid system in mouse spinal cord and induces opioid receptor tolerance., PMID:15140929
Prolonged kappa opioid receptor phosphorylation mediated by G-protein receptor kinase underlies sustained analgesic tolerance., PMID:14597630
Acute treatment with the cyclic antidepressant desipramine, but not fluoxetine, increases membrane-associated G protein-coupled receptor kinases 2/3 in rat brain., PMID:12527474
G-protein coupled receptor kinase 2 and 3 expression in human detrusor cultured smooth muscle cells., PMID:11762794
Upregulation of G protein-linked receptor kinases with advancing age in rat aorta., PMID:11171671
Ligand-induced phosphorylation of anaphylatoxin receptors C3aR and C5aR is mediated by "G protein-coupled receptor kinases., PMID:10508278
Differential effects of CC chemokines on CC chemokine receptor 5 (CCR5) phosphorylation and identification of phosphorylation sites on the CCR5 carboxyl terminus., PMID:10085131
Phosducin, potential role in modulation of olfactory signaling., PMID:9020189
Identification by polymerase chain reaction of the G protein-coupled receptor kinases expressed in the mouse gonadotropic alpha T3-1 cell line., PMID:8756570
Monoclonal antibodies reveal receptor specificity among G-protein-coupled receptor kinases., PMID:8755530
Odorant receptors and desensitization proteins colocalize in mammalian sperm., PMID:8529092