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Anti-MCM3 Antibody (R1S79)

Catalog #:   RHD66501 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: IF, IHC, WB
Accession: P25205
Overview

Catalog No.

RHD66501

Species reactivity

Human, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

IF: 1:50-1:200, IHC: 1:50-1:100, WB: 1:500-1:1000

Target

p102, MCM3, DNA replication licensing factor MCM3, RLF subunit beta, DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P25205

Applications

IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.

Clone ID

R1S79

Data Image
  • Immunofluorescence
    Immunocytochemistry analysis of MCM3(green) in Hela using MCM3 antibody,and DAPI(blue)
  • Immunohistochemical
    Immunohistochemistry analysis of paraffin-embedded Human tonsil using MCM3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Western blot
    Western blot analysis of MCM3 in Jurkat, C6, Hela lysates using MCM3 antibody.
References

Role of proliferative markers in assessing recurrences in surgical margins of oral squamous cell carcinoma: A systematic review., PMID:38304496

Inter-component immunohistochemical assessment of proliferative markers in uterine carcinosarcoma., PMID:36238851

Glucagon-Like Peptide-2 Stimulates S-Phase Entry of Intestinal Lgr5+ Stem Cells., PMID:35218981

The Immunohistochemical Expression of MCM-3, -5, and -7 Proteins in the Uterine Fibroids., PMID:34449552

Human ORC/MCM density is low in active genes and correlates with replication time but does not delimit initiation zones., PMID:33683199

Comparison of TMA Technique and Routine Whole Slide Analysis in Evaluation of Proliferative Markers Expression in Laryngeal Squamous Cell Cancer., PMID:33144432

Minichromosome Maintenance Proteins MCM-3, MCM-5, MCM-7, and Ki-67 as Proliferative Markers in Adrenocortical Tumors., PMID:30842144

Effect of daidzein and equol on DNA replication in MCF-7 cells., PMID:29346578

Morphological analysis of testicles in cats with disorders of sex development., PMID:28525341

Expression of p53 and selected proliferative markers (Ki-67, MCM3, PCNA, and topoisomerase IIα) in borderline ovarian tumors: Correlation with clinicopathological features., PMID:28493257

GAK is phosphorylated by c-Src and translocated from the centrosome to chromatin at the end of telophase., PMID:28135906

The evaluation of Ki67, p53, MCM3 and PCNA immunoexpressions at the level of the dental follicle of impacted teeth, dentigerous cysts and keratocystic odontogenic tumors., PMID:27516012

Immunoexpression of Ki-67, MCM2, and MCM3 in Ameloblastoma and Ameloblastic Carcinoma and Their Correlations with Clinical and Histopathological Patterns., PMID:26823641

Comparative analysis of markers of cell proliferation in canine mast cell tumours according to current classifications., PMID:26172171

Immunohistochemical evaluation of pulmonary lymphangioleiomyomatosis., PMID:26026095

Construction and use of a Trichinella spiralis phage display library to identify the interactions between parasite and host enterocytes., PMID:23420409

Characterization of O-GlcNAc cycling and proteomic identification of differentially O-GlcNAcylated proteins during G1/S transition., PMID:22967762

Increase of Mcm3 and Mcm4 expression in cervical squamous cell carcinomas., PMID:21077471

Replication origins are already licensed in G1 arrested unfertilized sea urchin eggs., PMID:20153311

Minichromosome maintenance proteins are useful adjuncts to differentiate between benign and malignant melanocytic skin lesions., PMID:19389522

GANP suppresses DNA recombination, measured by direct-repeat beta-galactosidase gene construct, but does not suppress the type of recombination applying to immunoglobulin genes in mammalian cells., PMID:17903179

Identification of carboxyl-terminal MCM3 phosphorylation sites using polyreactive phosphospecific antibodies., PMID:17244605

Mouse pre-replicative complex proteins colocalise and interact with the centrosome., PMID:17157410

Recombinant Cdt1 induces rereplication of G2 nuclei in Xenopus egg extracts., PMID:15668171

Cancer-associated expression of minichromosome maintenance 3 gene in several human cancers and its involvement in tumorigenesis., PMID:15623617

Autoantibodies and autoantigens in the urine of SLE patients., PMID:15621578

A hypophosphorylated form of RPA34 is a specific component of pre-replication centers., PMID:15456845

Involvement of GANP in B cell activation in T cell-dependent antigen response., PMID:12885157

The expression of Ki-67, MCM3, and p27 defines distinct subsets of proliferating, resting, and differentiated cells., PMID:11745678

PU.1 is involved in the regulation of B lineage-associated and developmental stage-dependent expression of the germinal center-associated DNA primase GANP., PMID:11641399

P1, a high mobility group-like protein is depressed in human breast adenocarcinoma., PMID:11544097

Immunohistochemical detection of cell growth fraction in formalin-fixed and paraffin-embedded murine tissue., PMID:11395376

Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis., PMID:11046155

Stepwise regulated chromatin assembly of MCM2-7 proteins., PMID:10722676

MCM proteins are associated with RNA polymerase II holoenzyme., PMID:10454562

Identification of a novel MCM3-associated protein that facilitates MCM3 nuclear localization., PMID:9712829

Human protein MCM6 on HeLa cell chromatin., PMID:9516426

B24 protein stored in lampbrush spheres is involved in early cleavage in urodele amphibians., PMID:9433800

Mcm2 and Mcm3 are constitutive nuclear proteins that exhibit distinct isoforms and bind chromatin during specific cell cycle stages of Saccharomyces cerevisiae., PMID:9285827

Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs., PMID:9214647

Identification of two Xenopus laevis genes, xMCM2 and xCDC46, with sequence homology to MCM genes involved in DNA replication., PMID:8917078

Binding of human minichromosome maintenance proteins with histone H3., PMID:8798650

XMCM7, a novel member of the Xenopus MCM family, interacts with XMCM3 and colocalizes with it throughout replication., PMID:8816774

The possible involvement of replication-related proteins with a DEAD-box-like motif in cell-free DNA replication of Xenopus eggs., PMID:7626098

A human homologue of the yeast replication protein Cdc21. Interactions with other Mcm proteins., PMID:7601140

MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells., PMID:7760938

DNA polymerase alpha associated protein P1, a murine homolog of yeast MCM3, changes its intranuclear distribution during the DNA synthetic period., PMID:7925275

Identification of an amphibian oocyte nuclear protein as a candidate for a role in embryonic DNA replication., PMID:8179995

The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins., PMID:8265339

Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein., PMID:1549468

Datasheet

Document Download

Anti-MCM3 Antibody (R1S79).pdf

 

$ 290
Product specifications
50 μg 290 100 μg 484

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Anti-MCM3 Antibody (R1S79) [RHD66501]
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