Catalog No.
RHD02301
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IF: 1:50-1:200, IHC: 1:100-1:200, IP: 1:20-1:50, WB: 1:1000-1:2000
Target
Transcription initiation factor RAP30, GTF2F2, ATP-dependent helicase GTF2F2, General transcription factor IIF subunit 2, RAP30, Transcription initiation factor IIF subunit beta, TFIIF-beta, General transcription factor IIF 30 kDa subunit
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P13984
Applications
IF, IHC, IP, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1P19
Regulation of mammalian transcription by Gdown1 through a novel steric crosstalk revealed by cryo-EM., PMID:22850672
Purification and identification of proteins that bind to the hereditary persistence of fetal hemoglobin -198 mutation in the gamma-globin gene promoter., PMID:17114178
Interactions of the HIV-1 Tat and RAP74 proteins with the RNA polymerase II CTD phosphatase FCP1., PMID:15723517
Sequential Peptide Affinity (SPA) system for the identification of mammalian and bacterial protein complexes., PMID:15253427
TFIIF-associating carboxyl-terminal domain phosphatase dephosphorylates phosphoserines 2 and 5 of RNA polymerase II., PMID:12351650
Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II., PMID:11839823
PITSLRE p110 protein kinases associate with transcription complexes and affect their activity., PMID:11709559
Quantitation of the RNA polymerase II transcription machinery in yeast., PMID:11591727
Immunoaffinity purification of the RAP30 subunit of human transcription factor IIF., PMID:10545274
The binding of the alpha subunit of protein kinase CK2 and RAP74 subunit of TFIIF to protein-coding genes in living cells is DRB sensitive., PMID:10094404
Heat shock of HeLa cells inactivates a nuclear protein phosphatase specific for dephosphorylation of the C-terminal domain of RNA polymerase II., PMID:9973623
Autoantibody reactive with the human general transcription factor TFIIF in sera from patients with autoimmune disorders., PMID:9328127
Affinity purification of a human RNA polymerase II complex using monoclonal antibodies against transcription factor IIF., PMID:9111063
Regulation of CDK7 substrate specificity by MAT1 and TFIIH., PMID:9130709
Distinct domains of hTAFII100 are required for functional interaction with transcription factor TFIIF beta (RAP30) and incorporation into the TFIID complex., PMID:8758937
Initiation of transcription by RNA polymerase II is limited by melting of the promoter DNA in the region immediately upstream of the initiation site., PMID:7982911
Transcription of the human T-cell lymphotropic virus type I promoter by an alpha-amanitin-resistant polymerase., PMID:7521915
Functional analysis of Drosophila factor 5 (TFIIF), a general transcription factor., PMID:8175788
The carboxyl terminus of RAP30 is similar in sequence to region 4 of bacterial sigma factors and is required for function., PMID:1429731
Factors involved in specific transcription by mammalian RNA polymerase II. RNA polymerase II-associating protein 30 is an essential component of transcription factor IIF., PMID:3392044
For research use only. Not for human or drug use.
