Catalog No.
RHC78001
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Applications
IF, WB
Recommended Dilution
| Application |
Dilution |
| IF |
1:50-1:200, WB: 1:1000-1:2000 |
Target
GLI3 form of 190 kDa, GLI3FL, GLI3-83, Transcriptional activator GLI3, GLI3, GLI3-190, GLI3 C-terminally truncated form, GLI3 full-length protein, GLI3 form of 83 kDa
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P10071
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1M54
Note
For research use only.
Sonic hedgehog regulates the growth and patterning of the cerebellum., PMID:10375501
The Shh signalling pathway in early tooth development., PMID:10512189
Mouse suppressor of fused is a negative regulator of sonic hedgehog signaling and alters the subcellular distribution of Gli1., PMID:10531011
Hedgehog-regulated processing of Gli3 produces an anterior/posterior repressor gradient in the developing vertebrate limb., PMID:10693759
Ski is involved in transcriptional regulation by the repressor and full-length forms of Gli3., PMID:12435627
Sonic hedgehog signaling regulates Gli3 processing, mesenchymal proliferation, and differentiation during mouse lung organogenesis., PMID:15136151
Sonic hedgehog expression and role in healing corneal epithelium., PMID:15277480
Inhibition of prostate cancer proliferation by interference with SONIC HEDGEHOG-GLI1 signaling., PMID:15314219
Hedgehog signaling pathway and gastric cancer., PMID:16258256
Increased apoptosis and accelerated epithelial migration following inhibition of hedgehog signaling in adaptive small bowel postresection., PMID:16439469
Functional compensation in Hedgehog signaling during mouse prostate development., PMID:16707121
Enhanced expression of hedgehog signaling molecules in squamous cell carcinoma of uterine cervix and its precursor lesions., PMID:16778829
Expression of homeotic genes Hoxa3, Hoxb3, Hoxd3 and Hoxc4 is decreased in the lungs but not in the hearts of adriamycin-exposed mice., PMID:17211587
A hypermorphic mouse Gli3 allele results in a polydactylous limb phenotype., PMID:17266131
Generation and characterization of mouse monoclonal antibody 5E1 against human transcription factor GLI3., PMID:17600494
Evc is a positive mediator of Ihh-regulated bone growth that localises at the base of chondrocyte cilia., PMID:17660199
Ucma--A novel secreted factor represents a highly specific marker for distal chondrocytes., PMID:17707622
Generation and characterization of mouse monoclonal antibody 5E1 against human transcription factor GLI3., PMID:17725385
Protein phosphatase 2A and rapamycin regulate the nuclear localization and activity of the transcription factor GLI3., PMID:18559511
Generation and characterization of a single-chain Fv antibody against G, a hedgehog signaling pathway transcription factor., PMID:18582209
Molecular specification and patterning of progenitor cells in the lateral and medial ganglionic eminences., PMID:18799682
Expression of sonic hedgehog signaling molecules in normal, hyperplastic and carcinomatous endometrium., PMID:19432668
Expression of hedgehog signaling molecules in Merkel cell carcinoma., PMID:19644931
Kinetics of hedgehog-dependent full-length Gli3 accumulation in primary cilia and subsequent degradation., PMID:20154143
Ofd1 is required in limb bud patterning and endochondral bone development., PMID:20920500
Genome-wide RNAi screen reveals disease-associated genes that are common to Hedgehog and Wnt signaling., PMID:21266715
MODY type 2 in Greig cephalopolysyndactyly syndrome (GCPS) as part of a contiguous gene deletion syndrome., PMID:22043488
Abrogation of Gli3 expression suppresses the growth of colon cancer cells via activation of p53., PMID:22227409
GLI3 constrains digit number by controlling both progenitor proliferation and BMP-dependent exit to chondrogenesis., PMID:22465667
Overexpression of the Hedgehog signalling pathway in head and neck squamous cell carcinoma., PMID:23689223
Anti-apoptotic role of the sonic hedgehog signaling pathway in the proliferation of ameloblastoma., PMID:23835807
Hedgehog signaling regulates telomerase reverse transcriptase in human cancer cells., PMID:24086482
Murine Joubert syndrome reveals Hedgehog signaling defects as a potential therapeutic target for nephronophthisis., PMID:24946806
Sonic hedgehog signalling proteins are frequently expressed in retinoblastoma and are associated with aggressive clinicopathological features., PMID:25296932
Hedgehog signaling stimulates the formation of proliferating Müller glia-derived progenitor cells in the chick retina., PMID:26116667
Measuring Expression Levels of Endogenous Gli Genes by Immunoblotting and Real-Time PCR., PMID:26179041
Sonic Hedgehog regulates thymic epithelial cell differentiation., PMID:26778835
May Sonic Hedgehog proteins be markers for malignancy in uterine smooth muscle tumors?, PMID:26997437
Association of expression of the hedgehog signal with Merkel cell polyomavirus infection and prognosis of Merkel cell carcinoma., PMID:28551328
PKA-mediated Gli2 and Gli3 phosphorylation is inhibited by Hedgehog signaling in cilia and reduced in Talpid3 mutant., PMID:28673820
Centrosomal protein Dzip1l binds Cby, promotes ciliary bud formation, and acts redundantly with Bromi to regulate ciliogenesis in the mouse., PMID:29487109
The SHH/Gli axis regulates CD90-mediated liver cancer stem cell function by activating the IL6/JAK2 pathway., PMID:29722127
New insights into the GDF9-Hedgehog-GLI signaling pathway in human ovaries: from fetus to postmenopause., PMID:33772413
Distinct Whole Transcriptomic Profiles of the Bursa of Fabricius in Muscovy Ducklings Infected by Novel Duck Reovirus with Different Virulence., PMID:36680150
For research use only. Not for human or drug use.
