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Anti-Phospho-EIF4E/eIF4E (Ser209) Antibody (R1K72)

Catalog #:   RHC20902 Specific References (4) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: IF, IHC, IP, WB
Accession: P06730
Overview

Catalog No.

RHC20902

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

IF: 1:50-1:200, IHC: 1:50-1:100, IP: 1:20, WB: 1:500-1:1000

Target

Eukaryotic translation initiation factor 4E, EIF4F, mRNA cap-binding protein, eIF4E, eIF-4E, eIF-4F 25 kDa subunit, EIF4EL1, EIF4E

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P06730

Applications

IF, IHC, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.

Clone ID

R1K72

Data Image
  • Immunofluorescence
    Immunocytochemistry analysis of eIF4E (Phospho-Ser209)(green) in Hela using eIF4E (Phospho-Ser209) antibody,and DAPI(blue)
  • Immunohistochemical
    Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Phospho-eIF4E (Ser209) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Western blot
    Western blot analysis of eIF4E (Phospho-Ser209) in K562, rat Brain, C6, 3T3 lysates using Phospho-eIF4E (Ser209) antibody.
References

Tirbanibulin decreases cell proliferation and downregulates protein expression of oncogenic pathways in human papillomavirus containing HeLa cells., PMID:38967656

Phospho-eIF4E stimulation regulates coronavirus entry by selective expression of cell membrane-residential factors., PMID:38299843

Overexpression of phospho-eIF4E is associated with survival through AKT pathway in non-small cell lung cancer., PMID:20008839

Sorafenib and rapamycin induce growth suppression in mouse models of hepatocellular carcinoma., PMID:19220580

Datasheet
$ 290
Product specifications
50 μg 290 100 μg 484

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-Phospho-EIF4E/eIF4E (Ser209) Antibody (R1K72) [RHC20902]
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