Catalog No.
RHC11202
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IHC: 1:100-1:200, WB: 1:1000-1:2000
Target
GNAI2B, GNAI2, Guanine nucleotide-binding protein G(i) subunit alpha-2, Adenylate cyclase-inhibiting G alpha protein
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P04899
Applications
IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1J78
Targeting Gαi2 in neutrophils protects from myocardial ischemia reperfusion injury., PMID:38811421
GNAI1 and GNAI3 Reduce Colitis-Associated Tumorigenesis in Mice by Blocking IL6 Signaling and Down-regulating Expression of GNAI2., PMID:30836096
Insulin secretion stimulated by L-arginine and its metabolite L-ornithine depends on Gα(i2)., PMID:25205820
The loss of Gnai2 and Gnai3 in B cells eliminates B lymphocyte compartments and leads to a hyper-IgM like syndrome., PMID:23977324
Colitis immunoregulation by CD8+ T cell requires T cell cytotoxicity and B cell peptide antigen presentation., PMID:18617557
TL1A (TNFSF15) regulates the development of chronic colitis by modulating both T-helper 1 and T-helper 17 activation., PMID:18598698
An obligatory requirement for the heterotrimeric G protein Gi3 in the antiautophagic action of insulin in the liver., PMID:17296938
The -318 C>G single-nucleotide polymorphism in GNAI2 gene promoter region impairs transcriptional activity through specific binding of Sp1 transcription factor and is associated with high blood pressure in Caucasians from Italy., PMID:16565233
Long-term treatment with anti-alpha 4 integrin antibodies aggravates colitis in G alpha i2-deficient mice., PMID:16052630
Expression of SphK1 impairs degranulation and motility of RBL-2H3 mast cells by desensitizing S1P receptors., PMID:15741218
Macrophages induce the inflammatory response in the pulmonary Arthus reaction through G alpha i2 activation that controls C5aR and Fc receptor cooperation., PMID:15728518
Dietary antigen-specific T-cell responses: switch from an interleukin-10-dominated response in normal mice to a T-helper 1 cytokine profile in Galphai2-deficient mice prior to colitis., PMID:15644120
Heterologous desensitization of insulin secretion by GIP (glucose-dependent insulinotropic peptide) in INS-1 cells: the significance of Galphai2 and investigations on the mechanism involved., PMID:15376236
ACTH stimulates the release of alkaline phosphatase through Gi-mediated activation of a phospholipase C and the release of inositol-phosphoglycan., PMID:15030184
SDF-1/CXCL12 regulates cAMP production and ion transport in intestinal epithelial cells via CXCR4., PMID:14684377
Vomeronasal phenotype and behavioral alterations in G alpha i2 mutant mice., PMID:12867032
Calbindin D28K immunoreactive neurons in vomeronasal organ and their projections to the accessory olfactory bulb in the rat., PMID:12834886
B cell developmental requirement for the G alpha i2 gene., PMID:12574334
Matrix metalloproteinase-1 activates a pertussis toxin-sensitive signaling pathway that stimulates the release of matrix metalloproteinase-9., PMID:12358794
Opposing changes of trimeric G protein levels during ontogenetic development of rat brain., PMID:11850064
Antibody response to dietary and autoantigens in G alpha i2-deficient mice., PMID:11742190
Somatostatin inhibits exocytosis in rat pancreatic alpha-cells by G(i2)-dependent activation of calcineurin and depriming of secretory granules., PMID:11533141
Clonal composition of human adamantinomatous craniopharyngiomas and somatic mutation analyses of the patched (PTCH), Gsalpha and Gi2alpha genes., PMID:11524144
Immunocytochemical study of G(i)2alpha and G(o)alpha on the epithelium surface of the rat vomeronasal organ., PMID:11238246
Identification of galpha subtype(s) involved in gamma-aminobutyric acid(B) receptor-mediated high-affinity guanosine triphosphatase activity in rat cerebral cortical membranes., PMID:11121889
Positive regulation of human T cell activation by Gi2 proteins and interleukin-8., PMID:10811016
A rho exchange factor mediates thrombin and Galpha(12)-induced cytoskeletal responses., PMID:10480888
Identification of Galpha13 as one of the G-proteins that couple to human platelet thromboxane A2 receptors., PMID:10318854
Identification and localization of G protein subunits in human spermatozoa., PMID:10050660
Influence of Gz and Gi2 transducer proteins in the affinity of opioid agonists to mu receptors., PMID:9767386
Angiotensin II modulates ANP-R2/ANP-C receptor-mediated inhibition of adenylyl cyclase in vascular smooth muscle cells: role of protein kinase C., PMID:9737934
The heterotrimeric G protein G alpha i2 mediates lysophosphatidic acid-stimulated induction of the c-fos gene in mouse fibroblasts., PMID:8995363
Ulcerative colitis and adenocarcinoma of the colon in G alpha i2-deficient mice., PMID:7663509
Targeting of G alpha i2 to the Golgi by alternative spliced carboxyl-terminal region., PMID:8272874
For research use only. Not for human or drug use.
