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Anti-EGFR/ERBB1/HER1 Antibody (R1H32)

Catalog #:   RHB86913 Specific References (13) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: IF, IHC, IP, WB
Accession: P00533
Overview

Catalog No.

RHB86913

Species reactivity

Human, Mouse

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Applications

IF, IHC, IP, WB

Recommended Dilution

Application Dilution
IF 1:50-1:200, IHC: 1:50-1:100, IP: 1:20, WB: 1:500-1:1000

Target

HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P00533

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.

Clone ID

R1H32

Note

For research use only.

Data Image
  • Immunofluorescence
    Immunocytochemistry analysis of EGFR (green) in 3T3 using EGFR antibody,and DAPI(blue).
  • Immunohistochemical
    Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using EGFR antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Western blot
    Western blot analysis of EGFR in Hela lysates using EGFR antibody.
References

Estrogen receptor, epidermal growth factor receptor and cellular ploidy in elutriated subpopulations of hepatocytes during liver tumor promotion by 17 alpha-ethinylestradiol in rats., PMID:1672626

Attachment of A-431 cells on immobilized antibodies to the EGF receptor promotes cell spreading and reorganization of the microfilament system., PMID:11124708

Cetuximab and irinotecan/5-fluorouracil/folinic acid is a safe combination for the first-line treatment of patients with epidermal growth factor receptor expressing metastatic colorectal carcinoma., PMID:16303861

Tyrosine kinase of insulin-like growth factor receptor as target for novel treatment and prevention strategies of colorectal cancer., PMID:17007015

Complications after oesophagectomy with possible contribution of neoadjuvant therapy including an EGFR-antibody to a fatal outcome., PMID:17927839

Treatment of metastatic colorectal cancer with cetuximab: influence on the quality of life., PMID:23955138

KRAS and aneusomy of chromosomes 4, 10 and 12 in colorectal carcinomas., PMID:26122820

Epidermal growth factor receptor variant III in head and neck squamous cell carcinoma is not relevant for targeted therapy and irradiation., PMID:28427242

Amphiregulin (AREG) and Epiregulin (EREG) Gene Expression as Predictor for Overall Survival (OS) in Oxaliplatin/Fluoropyrimidine Plus Bevacizumab Treated mCRC Patients-Analysis of the Phase III AIO KRK-0207 Trial., PMID:30467535

Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck., PMID:31561451

Predictive and prognostic value of magnesium serum level in FOLFIRI plus cetuximab or bevacizumab treated patients with stage IV colorectal cancer: results from the FIRE-3 (AIO KRK-0306) study., PMID:32639280

EGFR High Copy Number Together With High EGFR Protein Expression Predicts Improved Outcome for Cetuximab-based Therapy in Squamous Cell Lung Cancer: Analysis From SWOG S0819, a Phase III Trial of Chemotherapy With or Without Cetuximab in Advanced NSCLC., PMID:34753703

Staudinger Reaction-Responsive Coacervates for Cytosolic Antibody Delivery and TRIM21-Mediated Protein Degradation., PMID:39805770

Datasheet
$ 290
Product specifications
50 μg 290 100 μg 484

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-EGFR/ERBB1/HER1 Antibody (R1H32) [RHB86913]
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