Catalog No.
RHB40401
Species reactivity
Human, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
WB: 1:1000-1:2000
Target
hRNF8, E3 ubiquitin-protein ligase RNF8, RING-type E3 ubiquitin transferase RNF8, RNF8, RING finger protein 8, KIAA0646
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
O76064
Applications
WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1F90
RNF8-ubiquitinated KMT5A is required for RNF168-induced H2A ubiquitination in response to DNA damage., PMID:33710666
DNA Repair Network Analysis Reveals Shieldin as a Key Regulator of NHEJ and PARP Inhibitor Sensitivity., PMID:29656893
Characterization of Cardiac Glycoside Natural Products as Potent Inhibitors of DNA Double-Strand Break Repair by a Whole-Cell Double Immunofluorescence Assay., PMID:26927829
Adenovirus-mediated downregulation of the ubiquitin ligase RNF8 sensitizes bladder cancer to radiotherapy., PMID:26788910
HTLV-1 Tax Stimulates Ubiquitin E3 Ligase, Ring Finger Protein 8, to Assemble Lysine 63-Linked Polyubiquitin Chains for TAK1 and IKK Activation., PMID:26285145
Ubiquitin-H2AX fusions render 53BP1 recruitment to DNA damage sites independent of RNF8 or RNF168., PMID:25695757
BCL10 regulates RNF8/RNF168-mediated ubiquitination in the DNA damage response., PMID:24732096
USP3 counteracts RNF168 via deubiquitinating H2A and γH2AX at lysine 13 and 15., PMID:24196443
Three-dimensional imaging reveals the spatial separation of γH2AX-MDC1-53BP1 and RNF8-RNF168-BRCA1-A complexes at ionizing radiation-induced foci., PMID:22633816
BMI1 is recruited to DNA breaks and contributes to DNA damage-induced H2A ubiquitination and repair., PMID:21383063
53BP1-dependent robust localized KAP-1 phosphorylation is essential for heterochromatic DNA double-strand break repair., PMID:20081839
The RNF8/RNF168 ubiquitin ligase cascade facilitates class switch recombination., PMID:20080757
A viral E3 ligase targets RNF8 and RNF168 to control histone ubiquitination and DNA damage responses., PMID:20075863
PTIP regulates 53BP1 and SMC1 at the DNA damage sites., PMID:19414588
For research use only. Not for human or drug use.
