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Anti-OGT Antibody (R1D69)

Catalog #:   RHA48402 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: FCM, IF, IHC, WB
Accession: O15294
Overview

Catalog No.

RHA48402

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

FCM: 1:20-1:100, IF: 1:50-1:200, IHC: 1:100-1:200, WB: 1:1000-1:2000

Target

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, OGT, O-linked N-acetylglucosamine transferase 110 kDa subunit, O-GlcNAc transferase subunit p110

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O15294

Applications

FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.

Clone ID

R1D69

Data Image
  • Western blot
    All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
  • Western blot
    All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
  • Western blot
    Western blot analysis of OGT / O-Linked N-Acetylglucosamine Transferase expression in A549 cell lysate.
References

Expression of O-GlcNAcylation in pulp tissue and dental pulp stem cells of healthy dental organs., PMID:40152124

PI3Kβ functions as a protein kinase to promote cellular protein O-GlcNAcylation and acetyl-CoA production for tumor growth., PMID:40132583

Safety, Tolerability, and Immunogenicity of the Pneumococcal Vaccines PPSV23 or PCV15 Co-Administered with a Booster Dose of mRNA-1273 SARS-CoV-2 Vaccine in Healthy Adults ≥50 Years of Age., PMID:40006738

O-GlcNAcylation of ribosome-associated proteins is concomitant with translational reprogramming during proteotoxic stress., PMID:39395807

Association between O-GlcNAc levels and platelet function in obese insulin-resistant subjects., PMID:39300054

Sequence variants influencing the regulation of serum IgG subclass levels., PMID:39277589

A randomized phase 1/2a trial of ExPEC10V vaccine in adults with a history of UTI., PMID:38877036

Cullin5 drives experimental asthma exacerbations by modulating alveolar macrophage antiviral immunity., PMID:38177117

Fructose-1,6-bisphosphatase 1 suppresses PPARα-mediated gene transcription and non-small-cell lung cancer progression., PMID:37970353

Safety, Reactogenicity, Immunogenicity, and Dose Selection of 10-Valent Extraintestinal Pathogenic Escherichia coli Bioconjugate Vaccine (VAC52416) in Adults Aged 60-85 Years in a Randomized, Multicenter, Interventional, First-in-Human, Phase 1/2a Study., PMID:37608916

A novel shark single-domain antibody targeting OGT as a tool for detection and intracellular localization., PMID:36855630

Ac34FGlcNAz is an effective metabolic chemical reporter for O-GlcNAcylated proteins with decreased S-glyco-modification., PMID:36610251

Immuno-PET Monitoring of Lymphocytes Using the CD8-Specific Antibody REGN5054., PMID:35895745

How Nanotechniques Could Vitalize the O-GlcNAcylation-Targeting Approach for Cancer Therapy., PMID:35498390

The Metabolic Chemical Reporter Ac46AzGal Could Incorporate Intracellular Protein Modification in the Form of UDP-6AzGlc Mediated by OGT and Enzymes in the Leloir Pathway., PMID:34712646

Novel Antibodies for the Simple and Efficient Enrichment of Native O-GlcNAc Modified Peptides., PMID:34678516

Regulation of O-Linked N-Acetyl Glucosamine Transferase (OGT) through E6 Stimulation of the Ubiquitin Ligase Activity of E6AP., PMID:34638625

Orengedokuto exerts anti-allergic effects via inhibition of effector T cell activation in a murine model of contact hypersensitivity., PMID:34510369

Generation of T-cell-redirecting bispecific antibodies with differentiated profiles of cytokine release and biodistribution by CD3 affinity tuning., PMID:34257348

Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine., PMID:34004049

New Insights Into the Biology of Protein O-GlcNAcylation: Approaches and Observations., PMID:35822169

The Dysregulation of OGT/OGA Cycle Mediates Tau and APP Neuropathology in Down Syndrome., PMID:33258073

C-Terminal Tag Location Hampers in Vitro Profiling of OGT Peptide Substrates by mRNA Display., PMID:33022805

Engineering a Proximity-Directed O-GlcNAc Transferase for Selective Protein O-GlcNAcylation in Cells., PMID:32119511

Fatty acid-induced CD36 expression via O-GlcNAcylation drives gastric cancer metastasis., PMID:31410220

DR4-Ser424 O-GlcNAcylation Promotes Sensitization of TRAIL-Tolerant Persisters and TRAIL-Resistant Cancer Cells to Death., PMID:30987996

Ac4GlcNAcF3, an OGT-tolerated but OGA-resistant regulator for O-GlcNAcylation., PMID:30713024

Production of Glycopeptide Derivatives for Exploring Substrate Specificity of Human OGA Toward Sugar Moiety., PMID:30693278

Detecting and Imaging O-GlcNAc Sites Using Glycosyltransferases: A Systematic Approach to Study O-GlcNAc., PMID:30100348

MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression., PMID:29720943

O-GlcNAcylation in oral squamous cell carcinoma., PMID:29327476

O-GlcNAcylation is required for B cell homeostasis and antibody responses., PMID:29187734

Single Quantum Dot-Based Nanosensor for Sensitive Detection of O-GlcNAc Transferase Activity., PMID:29115822

Checkpoint kinase 1-induced phosphorylation of O-linked β-N-acetylglucosamine transferase regulates the intermediate filament network during cytokinesis., PMID:29021254

Characterization of tools to detect and enrich human and mouse O-GlcNAcase., PMID:28595377

New ELISA-based method for the detection of O-GlcNAc transferase activity in vitro., PMID:28296566

Fatty acid synthase inhibits the O-GlcNAcase during oxidative stress., PMID:28232487

O-GlcNAc transferase regulates excitatory synapse maturity., PMID:28143929

O-Linked N-Acetylglucosamine (O-GlcNAc) Expression Levels Epigenetically Regulate Colon Cancer Tumorigenesis by Affecting the Cancer Stem Cell Compartment via Modulating Expression of Transcriptional Factor MYBL1., PMID:28096468

Genome-wide chemical mapping of O-GlcNAcylated proteins in Drosophila melanogaster., PMID:27918560

Functional analysis of recombinant human and Yarrowia lipolytica O-GlcNAc transferases expressed in Saccharomyces cerevisiae., PMID:27687229

Comparison of three different methods for the quantification of equine insulin., PMID:27613127

O-GlcNAc modification of Sp3 and Sp4 transcription factors negatively regulates their transcriptional activities., PMID:26431879

Diet-Induced Podocyte Dysfunction in Drosophila and Mammals., PMID:26190114

O-GlcNAcylation of the human epidermal growth factor receptor., PMID:26108188

Autoantibody profile of patients infected with knowlesi malaria., PMID:26086445

Inhibition of O-Linked N-Acetylglucosamine Transferase Reduces Replication of Herpes Simplex Virus and Human Cytomegalovirus., PMID:26041297

Diabetes-associated dysregulation of O-GlcNAcylation in rat cardiac mitochondria., PMID:25918408

Glucosamine-induced OGT activation mediates glucose production through cleaved Notch1 and FoxO1, which coordinately contributed to the regulation of maintenance of self-renewal in mouse embryonic stem cells., PMID:24730386

Antibodies that detect O-linked β-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins., PMID:24573683

Datasheet

Document Download

Anti-OGT Antibody (R1D69).pdf

 

$ 290
Product specifications
50 μg 290 100 μg 484

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Anti-OGT Antibody (R1D69) [RHA48402]
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