Catalog No.
RHA29001
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
WB: 1:500-1:1000
Target
Cell cycle checkpoint kinase, Serine/threonine-protein kinase Chk1, Checkpoint kinase-1, CHK1, CHK1 checkpoint homolog, CHEK1
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
O14757
Applications
WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1D26
Co-inhibition of Notch1 and ChK1 triggers genomic instability and melanoma cell death increasing the lifespan of mice bearing melanoma brain metastasis., PMID:40437523
Uncovering miRNA-mRNA Regulatory Networks Related to Olaparib Resistance and Resensitization of BRCA2MUT Ovarian Cancer PEO1-OR Cells with the ATR/CHK1 Pathway Inhibitors., PMID:38786089
The luciferase-based in vivo protein-protein interaction assay revealed that CHK1 promotes PP2A and PME-1 interaction., PMID:38588804
AMT-562, a Novel HER3-targeting Antibody-Drug Conjugate, Demonstrates a Potential to Broaden Therapeutic Opportunities for HER3-expressing Tumors., PMID:37302522
Targeting CLDN6 in germ cell tumors by an antibody-drug-conjugate and studying therapy resistance of yolk-sac tumors to identify and screen specific therapeutic options., PMID:36991316
Inferring tumor-specific cancer dependencies through integrating ex vivo drug response assays and drug-protein profiling., PMID:35994503
CXCL2-mediated ATR/CHK1 signaling pathway and platinum resistance in epithelial ovarian cancer., PMID:34474677
Determination of CHK1 Cellular Localization by Immunofluorescence Microscopy., PMID:33786781
In search for biomarkers and potential drug targets for uterine serous endometrial cancer., PMID:33754208
Targeting IGF Perturbs Global Replication through Ribonucleotide Reductase Dysfunction., PMID:33509941
Mutations in DNA Repair Genes and Clinical Outcomes of Patients With Metastatic Colorectal Cancer Receiving Oxaliplatin or Irinotecan-containing Regimens., PMID:33298767
Precision medicine phase II study evaluating the efficacy of a double immunotherapy by durvalumab and tremelimumab combined with olaparib in patients with solid cancers and carriers of homologous recombination repair genes mutation in response or stable after olaparib treatment., PMID:32778095
Targeting Topoisomerase I in the Era of Precision Medicine., PMID:31227499
RPA-coated single-stranded DNA promotes the ETAA1-dependent activation of ATR., PMID:30975033
CHK1 dosage in germinal center B cells controls humoral immunity., PMID:30894677
A common Chk1-dependent phenotype of DNA double-strand break suppression in two distinct radioresistant cancer types., PMID:30607635
Expression, activation and clinical relevance of CHK1 and CHK2 in metastatic high-grade serous carcinoma., PMID:29804637
Checkpoint kinase 1-induced phosphorylation of O-linked β-N-acetylglucosamine transferase regulates the intermediate filament network during cytokinesis., PMID:29021254
Donor-Derived Metastatic Melanoma and Checkpoint Inhibition., PMID:28838438
Combining Chk1/2 Inhibition with Cetuximab and Radiation Enhances In Vitro and In Vivo Cytotoxicity in Head and Neck Squamous Cell Carcinoma., PMID:28138028
Loss of Glis2/NPHP7 causes kidney epithelial cell senescence and suppresses cyst growth in the Kif3a mouse model of cystic kidney disease., PMID:27181777
DS-8201a, A Novel HER2-Targeting ADC with a Novel DNA Topoisomerase I Inhibitor, Demonstrates a Promising Antitumor Efficacy with Differentiation from T-DM1., PMID:27026201
Key mediators of somatic ATR signaling localize to unpaired chromosomes in spermatocytes., PMID:26209650
And-1 coordinates with Claspin for efficient Chk1 activation in response to replication stress., PMID:26082189
Checkpoint kinase 2 is required for efficient immunoglobulin diversification., PMID:25483076
ATR/Chk1/Smurf1 pathway determines cell fate after DNA damage by controlling RhoB abundance., PMID:25249323
Gemcitabine and CHK1 inhibition potentiate EGFR-directed radioimmunotherapy against pancreatic ductal adenocarcinoma., PMID:24838526
CH-01 is a hypoxia-activated prodrug that sensitizes cells to hypoxia/reoxygenation through inhibition of Chk1 and Aurora A., PMID:23597309
Global analysis of phosphorylation of tau by the checkpoint kinases Chk1 and Chk2 in vitro., PMID:23550703
Rad9, Rad17, TopBP1 and claspin play essential roles in heat-induced activation of ATR kinase and heat tolerance., PMID:23383325
Chk1 and Mps1 jointly regulate correction of merotelic kinetochore attachments., PMID:23321637
Sensitization of tumor to ²¹²Pb radioimmunotherapy by gemcitabine involves initial abrogation of G2 arrest and blocked DNA damage repair by interference with Rad51., PMID:23200172
Screening for novel LRRK2 inhibitors using a high-throughput TR-FRET cellular assay for LRRK2 Ser935 phosphorylation., PMID:22952710
CHK1 kinase activity assay., PMID:22941630
Epstein-Barr virus-encoded latent membrane protein 1 impairs G2 checkpoint in human nasopharyngeal epithelial cells through defective Chk1 activation., PMID:22761726
Human embryonic stem cells fail to activate CHK1 and commit to apoptosis in response to DNA replication stress., PMID:22553144
Disruption of murine mp29/Syf2/Ntc31 gene results in embryonic lethality with aberrant checkpoint response., PMID:22448250
Therapeutic targeting of Chk1 in NSCLC stem cells during chemotherapy., PMID:22117197
Methods for studying checkpoint kinases - Chk1., PMID:21870291
Parvovirus B19 infection of human primary erythroid progenitor cells triggers ATR-Chk1 signaling, which promotes B19 virus replication., PMID:21680529
Alterations of ATM and CADM1 in chromosomal 11q22.3-23.2 region are associated with the development of invasive cervical carcinoma., PMID:21643982
Nuclear Chk1 prevents premature mitotic entry., PMID:21628425
Sonic hedgehog signaling protects human hepatocellular carcinoma cells against ionizing radiation in an autocrine manner., PMID:21377281
Premature chromosome condensation induced by caffeine, 2-aminopurine, staurosporine and sodium metavanadate in S-phase arrested HeLa cells is associated with a decrease in Chk1 phosphorylation, formation of phospho-H2AX and minor cytoskeletal rearrangements., PMID:21347609
Nek1 kinase functions in DNA damage response and checkpoint control through a pathway independent of ATM and ATR., PMID:21301226
Ataxia telangiectasia and Rad3-related overexpressing cancer cells induce prolonged G₂ arrest and develop resistance to ionizing radiation., PMID:21294646
Synergistic effects of topoisomerase I inhibitor, SN38, on Fas-mediated apoptosis., PMID:21036702
Chk1-dependent constitutive phosphorylation of BLM helicase at serine 646 decreases after DNA damage., PMID:20719863
MCL-1 localizes to sites of DNA damage and regulates DNA damage response., PMID:20647761
Adenovirus 12 E4orf6 inhibits ATR activation by promoting TOPBP1 degradation., PMID:20566845
For research use only. Not for human or drug use.
