Catalog No.
RHK37101
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Applications
FCM, IF, IHC, WB
Recommended Dilution
| Application |
Dilution |
| FCM |
1:20-1:100, IF: 1:50-1:200, IHC: 1:100-1:200, WB: 1:1000-1:2000 |
Target
BBC3, bcl2 binding component 3, jfy1, puma, p53 up-regulated modulator of apoptosis, PUMA alpha, BBC3
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q9BXH1
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Store at +4 ℃ short term (1-2 weeks).
Store at -20 ℃ 12 months.
Store at -80 ℃ long term.
Clone ID
R1B94
Note
For research use only.
Two different adenylyl cyclases in brain distinguished by monoclonal antibodies., PMID:3338465
Puma(*)Mcl-1 interaction is not sufficient to prevent rapid degradation of Mcl-1., PMID:16007132
The secreted protease factor CPAF is responsible for degrading pro-apoptotic BH3-only proteins in Chlamydia trachomatis-infected cells., PMID:16940052
A new p38 MAP kinase-regulated transcriptional coactivator that stimulates p53-dependent apoptosis., PMID:17380123
Frequent loss of expression of the pro-apoptotic protein Bim in renal cell carcinoma: evidence for contribution to apoptosis resistance., PMID:17486061
Interaction of Helicobacter pylori with gastric epithelial cells is mediated by the p53 protein family., PMID:18343378
Involvement of p53-transactivated Puma in cisplatin-induced renal tubular cell death., PMID:18761355
Immunohistochemical detection of BH3-only proteins in ameloblastic tumors., PMID:18826384
Histone deacetylase inhibitors prevent p53-dependent and p53-independent Bax-mediated neuronal apoptosis through two distinct mechanisms., PMID:19261878
PUMA mediates EGFR tyrosine kinase inhibitor-induced apoptosis in head and neck cancer cells., PMID:19421143
Valsartan protects against ER stress-induced myocardial apoptosis via CHOP/Puma signaling pathway in streptozotocin-induced diabetic rats., PMID:21345370
PUMA-mediated intestinal epithelial apoptosis contributes to ulcerative colitis in humans and mice., PMID:21490394
Distinct cellular and therapeutic effects of obatoclax in rituximab-sensitive and -resistant lymphomas., PMID:21492126
Regulation of memory B-cell survival by the BH3-only protein Puma., PMID:21868573
Mechanical stretch induces the apoptosis regulator PUMA in vascular smooth muscle cells., PMID:22021910
Hyperthermia-enhanced TRAIL- and mapatumumab-induced apoptotic death is mediated through mitochondria in human colon cancer cells., PMID:22174016
Regulation of PUMA induced by mechanical stress in rat cardiomyocytes., PMID:22862895
Supercritical carbon dioxide extraction of aromatic turmerone from Curcuma longa Linn. induces apoptosis through reactive oxygen species-triggered intrinsic and extrinsic pathways in human hepatocellular carcinoma HepG2 cells., PMID:22946656
Multiple roles of p53-related pathways in somatic cell reprogramming and stem cell differentiation., PMID:22964580
Age-related susceptibility to apoptosis in human retinal pigment epithelial cells is triggered by disruption of p53-Mdm2 association., PMID:23139272
The BH3-only protein Puma plays an essential role in p53-mediated apoptosis of chronic lymphocytic leukemia cells., PMID:23517560
Pro-apoptotic TP53 homolog TAp63 is repressed via epigenetic silencing and B-cell receptor signalling in chronic lymphocytic leukaemia., PMID:24117128
PUMA regulates germ cell loss and primordial follicle endowment in mice., PMID:24859845
Embryonic exposure to carbendazim induces the transcription of genes related to apoptosis, immunotoxicity and endocrine disruption in zebrafish (Danio rerio)., PMID:25304545
β-Arrestin2 encourages inflammation-induced epithelial apoptosis through ER stress/PUMA in colitis., PMID:25354317
MicroRNAs miR-23a-3p, miR-23b-3p, and miR-149-5p Regulate the Expression of Proapoptotic BH3-Only Proteins DP5 and PUMA in Human Pancreatic β-Cells., PMID:27737950
Stabilization of the p53-DNA Complex by the Nuclear Protein Dmp1α., PMID:28406729
Treatment of Donor Rat Hearts Prior to Transplantation with FLIP (FADD-Like Interleukin Beta-Converting Enzyme (FLICE)-Like Inhibitory Protein) in Cardioplegic Solution Decreased Apoptosis at Thirty Minutes Post-transplantation and Decreased Total Tyrosine Phosphorylation Levels., PMID:29483486
CTCF knockout reveals an essential role for this protein during the zebrafish development., PMID:29723654
Restoring PUMA induction overcomes KRAS-mediated resistance to anti-EGFR antibodies in colorectal cancer., PMID:29755130
MicroRNAs Enable mRNA Therapeutics to Selectively Program Cancer Cells to Self-Destruct., PMID:30088967
Bendamustine alone or with rituximab modifies expression of apoptosis-regulating genes and proteins of CLL cells, depending on IGVH mutational status., PMID:30187811
Cabozantinib induces PUMA-dependent apoptosis in colon cancer cells via AKT/GSK-3β/NF-κB signaling pathway., PMID:31182761
Association Between DNA Damage Response, Fibrosis and Type I Interferon Signature in Systemic Sclerosis., PMID:33123169
Massive digital gene expression analysis reveals different predictive profiles for immune checkpoint inhibitor therapy between adenocarcinoma and squamous cell carcinoma of advanced lung cancer., PMID:35135489
For research use only. Not for human or drug use.
