Catalog No.
RHF95503
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IF: 1:50-1:200, IHC: 1:100-1:200, IP: 1:20-1:50, WB: 1:1000-1:5000
Target
ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK1, MP2K1, PRKMK1, kinase MEK1, MEK1/2
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q02750, P36507
Applications
IF, IHC, IP, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Store at +4 ℃ short term (1-2 weeks).
Store at -20 ℃ 12 months.
Store at -80 ℃ long term.
Clone ID
R1A76
MIF regulates T helper 17 cell differentiation by activating the p38 MAPK signaling pathway to drive the pathogenesis of EAP., PMID:40440956
The distinct effect between Amyloid β (1‒40) and Amyloid β (1‒42) on the TRAP-stimulated platelet activation in diabetes mellitus., PMID:40436763
Antiphospholipid antibodies inhibit the migration and invasion of trophoblast cells by suppressing the JNK/C-Jun/MMP1 signaling pathway., PMID:40410845
p38 MAPK-mediated upregulation of claudin-3 and claudin-4 by gemcitabine contributes to chemoresistance in ovarian cancer., PMID:40403836
Bimodal genomic approach predicting Semaphorin 7A (SEMA7A) as prognostic biomarker in adrenocortical carcinoma., PMID:40291702
MAPK Signaling and Angiopoietin-2 Contribute to Endothelial Permeability in Capillary Malformations., PMID:40235979
Selective USP7 Inhibition Synergizes with MEK1/2 Inhibitor to Enhance Immune Responses and Potentiate Anti-PD-1 Therapy in NRAS-Mutant Melanoma., PMID:40204067
PD-1 suppression enhances HIV reactivation and T-cell immunity via MAPK/NF-κB signaling., PMID:40186291
Therapeutic potential of phytocompounds in rheumatoid arthritis: Molecular insights and clinical applications., PMID:40174276
Humanizing a CD28 signaling domain affects CD8 activation, exhaustion and stem-like precursors., PMID:40161835
The impact of signaling pathways on the desmosome ultrastructure in pemphigus., PMID:39882246
E203K mutation in MAP2K1 (MEK1) causes acquired resistance to PD-1 blockade but responds to trametinib: a case report., PMID:39806853
Differential substrate specificity of ERK, JNK, and p38 MAP kinases toward connexin 43., PMID:39798878
Mechanisms underlying the effects of the conditional knockdown of hepatic PCSK9 in attenuating lipopolysaccharide-induced acute liver inflammation., PMID:39716700
Two-sided function of osteopontin during osteoblast differentiation., PMID:39658208
Durvalumab and T-DXd Synergistically Promote Apoptosis of Cholangiocarcinoma Cells by Downregulating EGR1 Expression Through Inhibiting P38 MAPK Pathway., PMID:39607471
Antimicrobial Peptide Pro10-1D Exhibits Anti-Allergic Activity: A Promising Therapeutic Candidate., PMID:39596204
IQGAP1 promotes early B cell development, is essential for the development of marginal zone (MZ) B cells, and is critical for both T-dependent and T-independent antibody responses., PMID:39585462
Activation of mixed lineage kinase 3 by fine particulate matter induces skin inflammation in human keratinocytes., PMID:39547318
Pim1 is Critical in T-cell-independent B-cell Response and MAPK Activation in B Cells., PMID:39505064
Concurrent SOS1 and MEK suppression inhibits signaling and growth of NF1-null melanoma., PMID:39488215
Involvement of mitogen- and stress-activated protein kinase 1 in BMP-6-induced chondrocyte differentiation., PMID:39307301
Confined copper depletion via a hydrogel platform for reversing dabrafenib/cetuximab resistance in BRAFV600E-mutant colorectal cancer., PMID:39306044
Syk inhibitors reduce tau protein phosphorylation and oligomerization., PMID:39233131
Turmeric extract alleviates airway inflammation via oxidative stress-driven MAPKs/MMPs pathway., PMID:39216235
The oncogenic kinase TOPK upregulates in psoriatic keratinocytes and contributes to psoriasis progression by regulating neutrophils infiltration., PMID:39090602
Immunomodulatory Effect of Cordyceps militaris Polysaccharide on RAW 264.7 Macrophages by Regulating MAPK Signaling Pathways., PMID:39064986
The Evolving Role of Bruton's Tyrosine Kinase Inhibitors in B Cell Lymphomas., PMID:39062757
Evaluation of antitumor potential of an anti-glypican-1 monoclonal antibody in preclinical lung cancer models reveals a distinct mechanism of action., PMID:38966167
Complement regulatory protein CD46 promotes bladder cancer metastasis through activation of MMP9., PMID:38847230
Off-target effect of high-dose sildenafil on adenosine 5'- diphosphate and collagen-induced platelet activation through mitogen-activated protein kinase pathway in treated BALB/C mice and in vitro experiments: A preliminary study., PMID:38808925
Polyphyllin I ameliorates gefitinib resistance and inhibits the VEGF/VEGFR2/p38 pathway by targeting HIF-1a in lung adenocarcinoma., PMID:38761523
TLR2-ERK signaling pathway regulates expression of galectin-3 in a murine model of OVA-induced allergic airway inflammation., PMID:38754639
Promotion of neurite outgrowth by 3,5,7,3',4'-pentamethoxyflavone is mediated through ERK signaling pathway in Neuro2a cells., PMID:38662302
Binimetinib in combination with nivolumab or nivolumab and ipilimumab in patients with previously treated microsatellite-stable metastatic colorectal cancer with RAS mutations in an open-label phase 1b/2 study., PMID:38600471
A novel natural Syk inhibitor suppresses IgE-mediated mast cell activation and passive cutaneous anaphylaxis., PMID:38569323
Expression profile of mRNAs and miRNAs related to mitogen-activated kinases in HaCaT cell culture treated with lipopolysaccharide a and adalimumab., PMID:38557266
Tanshinone IIA induces ER stress and JNK activation to inhibit tumor growth and enhance anti-PD-1 immunotherapy in non-small cell lung cancer., PMID:38537440
Nanodrug-bacteria conjugates-mediated oncogenic collagen depletion enhances immune checkpoint blockade therapy against pancreatic cancer., PMID:38521069
Combined KRAS-MAPK pathway inhibitors and HER2-directed drug conjugate is efficacious in pancreatic cancer., PMID:38509064
Undaria pinnatifida ameliorates nasal inflammation by inhibiting eosinophil and mast cell activation and modulating the NF-κB/MAPKs signaling pathway., PMID:38488697
Anti-PD-(L)1 plus BRAF/MEK inhibitors (triplet therapy) after failure of immune checkpoint inhibition and targeted therapy in patients with advanced melanoma., PMID:38484692
A phase 1 trial of the MEK inhibitor selumetinib in combination with pembrolizumab for advanced or metastatic solid tumors., PMID:38483782
Phosphorylated p38 MAP kinase expression by leucocytes is increased in allergic humans and associated with IgE responses., PMID:38441376
The p38 MAPK/snail signaling axis participates in cadmium-induced lung cancer cell migration and invasiveness., PMID:38436850
Reporter cell lines to screen for inhibitors or regulators of the KRAS-RAF-MEK1/2-ERK1/2 pathway., PMID:38381045
Immunotherapy-resistant acute lymphoblastic leukemia cells exhibit reduced CD19 and CD22 expression and BTK pathway dependency., PMID:38376944
Targeting PI3K/AKT/mTOR and MAPK Signaling Pathways in Gastric Cancer., PMID:38339127
LOGGIC/FIREFLY-2: a phase 3, randomized trial of tovorafenib vs. chemotherapy in pediatric and young adult patients with newly diagnosed low-grade glioma harboring an activating RAF alteration., PMID:38291372
Efficacy and safety of 'Second Adjuvant' therapy with BRAF/MEK inhibitors after local therapy for recurrent melanoma following adjuvant PD-1 based immunotherapy., PMID:38278009
For research use only. Not for human or drug use.
