Catalog No.
KXX19401
Description
E. coli host cell proteins (HCP) constitute a diverse and functionally intricate group of macromolecules within the Escherichia coli cell. The host proteins of E. coli play crucial roles in various cellular processes. These proteins are involved in fundamental metabolic pathways such as glycolysis, the tricarboxylic acid cycle, and amino acid biosynthesis, which are essential for the survival and growth of the bacterium. Recombinant expression in E. coli is a simple and cost-effective method for producing certain proteins. These proteins, often used as therapeutic agents, must be highly purified. The manufacturing process may introduce contamination by HCPs from E. coli, which can reduce efficacy and cause adverse reactions. Therefore, minimizing HCP impurities is crucial. This simple, sensitive, and fast OneStep ELISA is a powerful method for optimizing purification processes, process control, routine quality control, and product release testing.
Applications
Used for the quantitative determination of E. coli HCP concentration in biological products.
Specificity
E. coli Host Cell Proteins (HCP)
Detection method
Colorimetric
Sample type
Cell culture supernatant
Assay type
Quantitative (Sandwich)
Range
0.39 ng/mL – 25 ng/mL
Sensitivity
0.18 ng/ml
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <10%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Note
For research use only.
A comparative study of the human T-cell leukemia virus type 2 integrase expressed in and purified from Escherichia coli and Pichia pastoris., PMID:12135562
Fab-scFv fusion protein: an efficient approach to production of bispecific antibody fragments., PMID:12165442
Immobilized metal-ion affinity chromatography of recombinant Fab protein OPG C11 in the presence of EDTA-Mg(II)., PMID:12458952
Characterization and topology of the membrane domain Nqo10 subunit of the proton-translocating NADH-quinone oxidoreductase of Paracoccus denitrificans., PMID:12693950
One-step purification of 5-enolpyruvylshikimate-3-phosphate synthase enzyme from Mycobacterium tuberculosis., PMID:12699693
Di-diabody: a novel tetravalent bispecific antibody molecule by design., PMID:12969563
High-level expression and one-step purification of recombinant dengue virus type 2 envelope domain III protein in Escherichia coli., PMID:14680965
Rapid, high-throughput purification of HIV-1 integrase using microtiter plate technology., PMID:14711511
Isolation of recombinant antibodies against EspA and intimin of Escherichia coli O157:H7., PMID:15243046
Rapid purification of recombinant listeriolysin O (LLO) from Escherichia coli., PMID:16052337
Overproduction in Escherichia coli and purification of Epstein-Barr virus EBNA-1., PMID:16403648
Directional and direct cloning strategy for high-throughput generation of recombinant baculoviruses., PMID:17068961
Detection of Iss and Bor on the surface of Escherichia coli., PMID:17309614
Construction of Escherichia coli dnaK-deletion mutant infected by lambdaDE3 for overexpression and purification of recombinant GrpE proteins., PMID:18434193
Identification of potential substrate proteins for the periplasmic Escherichia coli chaperone Skp., PMID:19003857
Tamavidin, a versatile affinity tag for protein purification and immobilization., PMID:20026208
Systems biology of recombinant protein production using Bacillus megaterium., PMID:21943898
Characterization of bacteriophages with a lytic effect on various Salmonella serotypes and Escherichia coli O157:H7., PMID:22145760
Efficient and versatile one-step affinity purification of in vivo biotinylated proteins: expression, characterization and structure analysis of recombinant human glutamate carboxypeptidase II., PMID:22178733
Double knockout of β-lactamase and cephalosporin acetyl esterase genes from Escherichia coli reduces cephalosporin C decomposition., PMID:22382016
Simultaneous clarification of Escherichia coli culture and purification of extracellularly produced penicillin G acylase using tangential flow filtration and anion-exchange membrane chromatography (TFF-AEMC)., PMID:22709629
Bacterial two hybrid: a versatile one-step intracellular selection method., PMID:22886250
GFP facilitates native purification of recombinant perlucin derivatives and delays the precipitation of calcium carbonate., PMID:23056388
Evaluation of cell-free expression system for the production of soluble and functional human GPCR N-formyl peptide receptors., PMID:23746112
Genomic investigation of lysogen formation and host lysis systems of the Salmonella temperate bacteriophage SPN9CC., PMID:24185850
Direct cadaverine production from cellobiose using β-glucosidase displaying Escherichia coli., PMID:24206923
An optimized protocol for overproduction of recombinant protein expression in Escherichia coli., PMID:24219068
Generation of human ER chaperone BiP in yeast Saccharomyces cerevisiae., PMID:24512104
Characterization, Genome Sequence, and Analysis of Escherichia Phage CICC 80001, a Bacteriophage Infecting an Efficient L-Aspartic Acid Producing Escherichia coli., PMID:26501200
Production of Double-stranded DNA Ministrings., PMID:26967586
Heparin-binding peptide as a novel affinity tag for purification of recombinant proteins., PMID:27235575
[Efficient expression of anthrax edema factor in Escherichia coli and its single-step purification]., PMID:29733175
Characterization of novel bacteriophage phiC119 capable of lysing multidrug-resistant Shiga toxin-producing Escherichia coli O157:H7., PMID:27672499
A high-throughput expression screening platform to optimize the production of antimicrobial peptides., PMID:28193216
Use of mariner transposases for one-step delivery and integration of DNA in prokaryotes and eukaryotes by transfection., PMID:28204586
Expression of Mastoparan B, a Venom Peptide, Via Escherichia coli C43 (DE3) Coupled with an Artificial Oil Body-Cyanogen Bromide Technology Platform., PMID:28741464
A versatile one-step CRISPR-Cas9 based approach to plasmid-curing., PMID:28764701
One-step affinity capture and precipitation for improved purification of an industrial monoclonal antibody using Z-ELP functionalized nanocages., PMID:28986999
Preparation of Tyrosylprotein Sulfotransferases for In Vitro One-Pot Enzymatic Synthesis of Sulfated Proteins/Peptides., PMID:30320268
One-Step Bacterial Artificial Chromosome (BAC) Modification: Preparation of Plasmids., PMID:32611776
One-Step Bacterial Artificial Chromosome (BAC) Modification: Transfer of the Reporter Vector into BAC/RecA Cells and Selection of Co-Integrates., PMID:32611780
Genetic Analysis and Characterization of a Bacteriophage ØCJ19 Active against Enterotoxigenic Escherichia coli., PMID:32968727
An Inducible Expression System for Recombinant Sca Proteins with an Autotransporter Domain from Orientia Tsutsugamushi in Escherichia coli., PMID:32972336
Identification of Escherichia coli Host Genes That Influence the Bacteriophage Lambda (λ) T4rII Exclusion (Rex) Phenotype., PMID:33033112
Characterization and in vitro activity of a lytic phage RDN37 isolated from community sewage water active against MDR Uropathogenic E. coli., PMID:33994226
Characterization of a novel group I F-specific RNA bacteriophage isolated from human stool., PMID:34050782
Tailoring Genetic Elements of the Plasmid-Driven T7 System for Stable and Robust One-Step Cloning and Protein Expression in Broad Escherichia coli., PMID:34597025
Production and applications of fluorobody from redox-engineered Escherichia coli., PMID:36729226
Isolation, Characterization, and Comparative Genomic Analysis of Bacteriophage Ec_MI-02 from Pigeon Feces Infecting Escherichia coli O157:H7., PMID:37298457
Isolation, Characterization, and Complete Genome Sequence of Escherichia Phage KIT06 Which Infects Nalidixic Acid-Resistant Escherichia coli., PMID:39061264
For research use only. Not for human or drug use.
