Catalog No.
KAV00310
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant RBD (KP.2) has been pre-coated onto a microplate. Standards or samples are premixed with HRP labeled ACE and then pipetted into the wells. Anti-RBD (KP.2) Neutralizing Antibody in the sample competitively binds to recombinant RBD (KP.2) with the HRP labeled ACE. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-RBD (KP.2) Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
Specificity
SARS-CoV-2 Surrogate Virus Neutralization Antibody (KP.2)
Applications
Used for the quantitative determination of Anti-RBD (KP.2) Neutralizing Antibody concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
131.69 - 1,500 ng/mL
Sensitivity
140.81 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <20%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <20%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
1437.5 |
849.1 |
413.2 |
1466.4 |
706.0 |
298.8 |
|
Standard deviation |
107.4 |
90.8 |
32.1 |
112.4 |
72.0 |
40.4 |
|
CV (%) |
7.5 |
10.7 |
7.8 |
7.7 |
10.2 |
13.5 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
For research use only. Not for human or drug use.
