Catalog No.
PHK09101
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human NEDD4L/Nedd4-2 (Glu629-Asp975).
Target
NEDD4L, E3 ubiquitin-protein ligase NEDD4-like, NEDD4.2, NEDL3, Nedd4-2, HECT-type E3 ubiquitin transferase NED4L, KIAA0439
Purification
Antigen affinity purification.
Accession
Q96PU5
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
NEDD4L binds the proteasome and promotes autophagy and bortezomib sensitivity in multiple myeloma., PMID:35236820
Glucocorticoids and serum- and glucocorticoid-inducible kinase 1 are potent regulators of CFTR in the native intestine: implications for stress-induced diarrhea., PMID:32567324
Label-free quantitative identification of abnormally ubiquitinated proteins as useful biomarkers for human lung squamous cell carcinomas., PMID:32140187
A potent voltage-gated calcium channel inhibitor engineered from a nanobody targeted to auxiliary CaVβ subunits., PMID:31403402
Tmbim1 is a multivesicular body regulator that protects against non-alcoholic fatty liver disease in mice and monkeys by targeting the lysosomal degradation of Tlr4., PMID:28481357
Targeting of PYK2 Synergizes with EGFR Antagonists in Basal-like TNBC and Circumvents HER3-Associated Resistance via the NEDD4-NDRG1 Axis., PMID:27793840
The Nedd4-2/Ndfip1 axis is a negative regulator of IgE-mediated mast cell activation., PMID:27786273
Ubiquitin ligase Nedd4-2 modulates Kv1.3 current amplitude and ion channel protein targeting., PMID:27146988
Renal tubular SGK1 deficiency causes impaired K+ excretion via loss of regulation of NEDD4-2/WNK1 and ENaC., PMID:27009335
Proteomics analysis of EV71-infected cells reveals the involvement of host protein NEDD4L in EV71 replication., PMID:25785312
Expression of ENaC, SGK1 and Nedd4 isoforms in the cochlea of guinea pig., PMID:25007182
The E3 ubiquitin ligase NEDD4 negatively regulates HER3/ErbB3 level and signaling., PMID:24662824
Nedd4-mediated increase in HIV-1 Gag and Env proteins and immunity following DNA-vaccination of BALB/c mice., PMID:24614057
AMP-activated protein kinase downregulates Kv7.1 cell surface expression., PMID:21957902
Inhibition of the heterotetrameric K+ channel KCNQ1/KCNE1 by the AMP-activated protein kinase., PMID:21231794
Protein kinase C-dependent ubiquitination and clathrin-mediated endocytosis of the cationic amino acid transporter CAT-1., PMID:21212261
Nedd4L modulates the transcription of metalloproteinase-1 and -13 genes to increase the invasive activity of gallbladder cancer., PMID:21039987
In vivo delivery of antigens by adenovirus dodecahedron induces cellular and humoral immune responses to elicit antitumor immunity., PMID:20179681
Lysine 63-linked polyubiquitination of the dopamine transporter requires WW3 and WW4 domains of Nedd4-2 and UBE2D ubiquitin-conjugating enzymes., PMID:20051513
Nedd4-2-mediated ubiquitination facilitates processing of surfactant protein-C., PMID:19423771
Expression patterns of Nedd4 isoforms and SGK1 in the rat cochlea., PMID:19051070
Cell survival through Trk neurotrophin receptors is differentially regulated by ubiquitination., PMID:16701206
14-3-3 isoforms are induced by aldosterone and participate in its regulation of epithelial sodium channels., PMID:16613846
Aldosterone-induced serum and glucocorticoid-induced kinase 1 expression is accompanied by Nedd4-2 phosphorylation and increased Na+ transport in cortical collecting duct cells., PMID:15958725
Nedd4-2 isoforms differentially associate with ENaC and regulate its activity., PMID:15814530
Nedd4-2 functionally interacts with ClC-5: involvement in constitutive albumin endocytosis in proximal tubule cells., PMID:15489223
A naturally occurring human Nedd4-2 variant displays impaired ENaC regulation in Xenopus laevis oocytes., PMID:15140763
Patch-clamp studies on epithelial sodium channels in salivary duct cells., PMID:12139396
Raft-partitioning of the ubiquitin ligases Cbl and Nedd4 upon IgE-triggered cell signaling., PMID:11248052
Cystic fibrosis transmembrane conductance regulator inhibits epithelial Na+ channels carrying Liddle's syndrome mutations., PMID:10318798
For research use only. Not for human or drug use.
