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Anti-Human SAE1 Polyclonal Antibody

Catalog #:   PHN34801 Specific References (40) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q9UBE0
Overview

Catalog No.

PHN34801

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SAE1 (Met1-Lys346).

Target

SAE1, AOS1, Ubiquitin-like 1-activating enzyme E1A, SUA1, SUMO-activating enzyme subunit 1, UBLE1A

Purification

Purified by antigen affinity column.

Accession

Q9UBE0

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with SAE1 antibody (PHN34801) at 1 µg/ml.

    Lane 1: Jurkat cell lysate
    Lane 2: MCF-7 cell lysate
    Lane 3: NIH3T3 cell lysate
    Lane 4: HT-29 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 38 kDa
    Observed MW: 38 kDa
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with SAE1 antibody (PHN34801) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: Human
    Observed MW:
References

Predictive value of myositis antibodies: role of semiquantitative classification and positivity for more than one autoantibody., PMID:39837620

Clinical features of anti-SAE1 antibody-positive myositis and interstitial lung disease: a multicenter, retrospective study in Taiwan., PMID:39575249

Juvenile dermatomyositis with Anti-SAE antibodies in a Moroccan child associated with pseudo-angioedema: a case report., PMID:38773611

Case report: A case of resistant anti-SAE1 dermatomyositis with severe periorbital edema after hydroxychloroquine that responded to adalimumab., PMID:38501604

Panniculitis on the trunk as a possible characteristic feature of anti-SAE1/2 antibody-positive dermatomyositis: A possible cutaneous manifestation of treatment resistance., PMID:38362647

Detection of Myositis Autoantibodies by Multi-Analytic Immunoassays in a Large Multicenter Cohort of Patients with Definite Idiopathic Inflammatory Myopathies., PMID:37835823

A case of anti-SAE1/2 antibody-positive dermatomyositis with extensive panniculitis: A possible cutaneous manifestation of treatment resistance., PMID:37830399

Trichoscopic, oral, and periungual fold findings as activity and damage markers in dermatomyositis patients and their correlation with myositis antibodies., PMID:36757439

Impaired muscle strength is associated with ultrastructure damage in myositis., PMID:36271295

Two clusters of systemic lupus erythematosus patients with muscle involvement in a Chinese cohort., PMID:36117395

Profile of specific and associated autoantibodies in patients with idiopathic inflammatory myopathies in a Colombian population., PMID:36045923

Cessation of Immunomodulatory Medication Use in Dermatomyositis: A Single-Center Cohort Study., PMID:35792485

Clinical and Autoantibody Associations in Antinuclear Antibody-Positive Systemic Sclerosis Lacking Prototypic Autoantibodies., PMID:35767831

Myositis-specific autoantibodies in clinical practice: Improving the performance of the immunodot., PMID:35427882

A Case of Anti-SAE1 Dermatomyositis., PMID:35280599

The Association of Myositis Specific Antibodies in Patients with Inflammatory Myositis: Preliminary Data in Indian Patients., PMID:34728950

Infliximab in a patient with treatment-resistant anti-SAE dermatomyositis., PMID:33221923

Frequencies and clinical associations of myositis-related antibodies in The Netherlands: A one-year survey of all Dutch patients., PMID:32743501

Extended myositis-specific and -associated antibodies profile in systemic sclerosis: A cross-sectional study., PMID:32653653

Analysis of myositis autoantibodies in Chinese patients with cancer-associated myositis., PMID:32222002

[Dermatomyositis: new antibody, new classification]., PMID:31859626

Distinct dermatomyositis populations are detected with different autoantibody assay platforms., PMID:31376258

SAE1 promotes human glioma progression through activating AKT SUMOylation-mediated signaling pathways., PMID:31345225

Diffuse pruritic erythema as a clinical manifestation in anti-SAE antibody-associated dermatomyositis: a case report and literature review., PMID:31030362

The spectrum and clinical significance of myositis-specific autoantibodies in Chinese patients with idiopathic inflammatory myopathies., PMID:30863950

Evaluating the diagnostic utility of new line immunoassays for myositis antibodies in clinical practice: a retrospective study., PMID:30840145

[Retrospective analysis of anti-TIF1gamma, anti-NXP2 and anti-SAE1/2 antibodies carriers at Bordeaux university hospital from November 2014 to February 2017]., PMID:30527961

Association Between Autoantibody Phenotype and Cutaneous Adverse Reactions to Hydroxychloroquine in Dermatomyositis., PMID:30140893

Anti-SAE Antibody-Positive Dermatomyositis in a Japanese Patient: A Case Report and Review of the Literature., PMID:30074913

Clinical significance of autoantibodies in dermatomyositis and systemic sclerosis., PMID:30047434

Diffuse erythema with 'angel wings' sign in Japanese patients with anti-small ubiquitin-like modifier activating enzyme antibody-associated dermatomyositis., PMID:30022478

Antibodies to small ubiquitin-like modifier activating enzyme are associated with a diagnosis of dermatomyositis: results from an unselected cohort., PMID:29845587

Dermatomyositis Clinical and Pathological Phenotypes Associated with Myositis-Specific Autoantibodies., PMID:29637414

[Dermatomyositis and Autoantibodies]., PMID:29632290

Prevalence of Myositis-Specific Antibodies in Idiopathic Interstitial Pneumonias., PMID:29532165

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity., PMID:29443041

Identification of multiple cancer-associated myositis-specific autoantibodies in idiopathic inflammatory myopathies: a large longitudinal cohort study., PMID:29178913

Specific autoantibodies in dermatomyositis: a helpful tool to classify different clinical subsets., PMID:27928683

Interstitial lung disease in an adult patient with dermatomyositis and anti-NXP2 autoantibody., PMID:26028648

Anti-SAE antibodies in autoimmune myositis: identification by unlabelled protein immunoprecipitation in an Italian patient cohort., PMID:22884621

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human SAE1 Polyclonal Antibody [PHN34801]
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