Anti-Human METTL3 Polyclonal Antibody

Catalog No.

PHJ15401

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human METTL3 (Met1-Leu580).

Target

METTL3, N6-adenosine-methyltransferase 70 kDa subunit, N6-adenosine-methyltransferase catalytic subunit, MTA70, Methyltransferase-like protein 3, MT-A70, hMETTL3

Purification

Purified by antigen affinity column.

Accession

Q86U44

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

METTL3 inhibition restores PD-L1 expression and CD8+ T-cell cytotoxic function in immunotherapy treated gastric cancer., PMID:40299705

Activated kynurenine pathway metabolism by YKL-40 establishes an inhibitory immune microenvironment and drives glioblastoma development., PMID:39718635

EZH2 promotes B-cell autoimmunity in primary Sjogren's syndrome via METTL3-mediated m6A modification., PMID:39577129

Clinicopathological analysis of primary central nervous system lymphoma in patients with or without HIV infection., PMID:39418717

Peptide Degrader-Based Targeting of METTL3/14 Improves Immunotherapy Response in Cutaneous Melanoma., PMID:39136347

Refining the role of N6-methyladenosine in cancer., PMID:39111230

M6A-mediated upregulation of lncRNA TUG1 in liver cancer cells regulates the antitumor response of CD8+ T cells and phagocytosis of macrophages., PMID:38981064

DHPS-Mediated Hypusination Regulates METTL3 Self-m6A-Methylation Modification to Promote Melanoma Proliferation and the Development of Novel Inhibitors., PMID:38952061

M6A RNA Methylation-Mediated Dysregulation of AGAP2-AS1 Promotes Trastuzumab Resistance of Breast Cancer., PMID:38744264

METTL3 promotes microglial inflammation via MEF2C in spinal cord injury., PMID:38180567

Methylation Regulation of LPCAT3 Improves Osteoarthritis by Regulating ACSL4 to Inhibit Chondrocyte Ferroptosis., PMID:38073444

METTL3-mediated HPV vaccine enhances the effect of anti PD-1 immunotherapy to alleviate the development of cutaneous squamous cell carcinoma., PMID:38030537

Development of a N6-methyladenosine-directed single quantum dot-based biosensor for sensitive detection of METTL3/14 complex activity in breast cancer tissues., PMID:37827689

METTL3 inhibition induced by M2 macrophage-derived extracellular vesicles drives anti-PD-1 therapy resistance via M6A-CD70-mediated immune suppression in thyroid cancer., PMID:37648786

METTL3 regulatory TROAP can regulate the progression of non-small cell lung cancer through PI3K/AKT and EMT signaling pathway., PMID:37608033

Adenosine Methylation Level of miR-125a-5p Promotes Anti-PD-1 Therapy Escape through the Regulation of IGSF11/VSIG3 Expression., PMID:37370798

Increased METTL3 expression and m6A RNA methylation may contribute to the development of dry eye in primary Sjögren's syndrome., PMID:37277716

m6A methyltransferase METTL3 programs CD4+ T-cell activation and effector T-cell differentiation in systemic lupus erythematosus., PMID:37013484

METTL3-Mediated N6-Methyladenosine Modification of lncRNA D26496 Suppresses the Proliferation and Migration of Schwann Cells after Sciatic Nerve Injury., PMID:36656457

High-precision mapping reveals rare N6-deoxyadenosine methylation in the mammalian genome., PMID:36575183

Antibody-free photoelectrochemical strategy for simultaneous detection of methylated RNA, METTL3/METTL14 protein and MazF protein based on enhanced photoactivity of MoSe2-BiOI nanocomposite., PMID:36529055

Adenosine N6-methylation upregulates the expression of human CYP2B6 by altering the chromatin status., PMID:36113565

METTL3 inhibition reduces N6 -methyladenosine levels and prevents allogeneic CD4+ T-cell responses., PMID:36005900

The methyltransferase METTL3 promotes tumorigenesis via mediating HHLA2 mRNA m6A modification in human renal cell carcinoma., PMID:35794583

LncRNA AC098934 promotes proliferation and invasion in lung adenocarcinoma cells by combining METTL3 and m6A modifications., PMID:35711823

METTL3 Inhibits Antitumor Immunity by Targeting m6A-BHLHE41-CXCL1/CXCR2 Axis to Promote Colorectal Cancer., PMID:35700773

Association Among the Gut Microbiome, the Serum Metabolomic Profile and RNA m6A Methylation in Sepsis-Associated Encephalopathy., PMID:35432460

Viral RNA N6-methyladenosine modification modulates both innate and adaptive immune responses of human respiratory syncytial virus., PMID:34929018

The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression., PMID:34893641

Stabilization of UCA1 by N6-methyladenosine RNA methylation modification promotes colorectal cancer progression., PMID:34809621

Baicalin suppresses the progression of Type 2 diabetes-induced liver tumor through regulating METTL3/m6A/HKDC1 axis and downstream p-JAK2/STAT1/clevaged Capase3 pathway., PMID:34763315

m6A RNA Immunoprecipitation Followed by High-Throughput Sequencing to Map N6-Methyladenosine., PMID:34694619

m6A modification impacts hepatic drug and lipid metabolism properties by regulating carboxylesterase 2., PMID:34536357

Mechanism of noncoding RNA-associated N6-methyladenosine recognition by an RNA processing complex during IgH DNA recombination., PMID:34450044

The germinal center reaction depends on RNA methylation and divergent functions of specific methyl readers., PMID:34402854

RNA Demethylase FTO Mediated RNA m6A Modification Is Involved in Maintaining Maternal-Fetal Interface in Spontaneous Abortion., PMID:34350169

Integrated analysis of m6A mRNA methylation in rats with monocrotaline-induced pulmonary arterial hypertension., PMID:34310344

The m6A landscape of polyadenylated nuclear (PAN) RNA and its related methylome in the context of KSHV replication., PMID:34187903

Deep and accurate detection of m6A RNA modifications using miCLIP2 and m6Aboost machine learning., PMID:34157120

Regulatory Mechanisms of the RNA Modification m6A and Significance in Brain Function in Health and Disease., PMID:34093133

Identification of m6A residues at single-nucleotide resolution using eCLIP and an accessible custom analysis pipeline., PMID:33376190

m6 A RNA methyltransferases METTL3/14 regulate immune responses to anti-PD-1 therapy., PMID:32964498

Modifications and interactions at the R-loop., PMID:32961406

Chidamide increases the sensitivity of Non-small Cell Lung Cancer to Crizotinib by decreasing c-MET mRNA methylation., PMID:32792859

ALKBH5 regulates anti-PD-1 therapy response by modulating lactate and suppressive immune cell accumulation in tumor microenvironment., PMID:32747553

Decreased Peripheral Blood ALKBH5 Correlates with Markers of Autoimmune Response in Systemic Lupus Erythematosus., PMID:32685056

Control of Early B Cell Development by the RNA N6-Methyladenosine Methylation., PMID:32610122

Epitranscriptomics in liver disease: Basic concepts and therapeutic potential., PMID:32330603

METTL3 regulates inflammatory pain by modulating m6A-dependent pri-miR-365-3p processing., PMID:31914601

Atlas of quantitative single-base-resolution N6-methyl-adenine methylomes., PMID:31822664

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Anti-Human METTL3 Polyclonal Antibody.pdf