Catalog No.
KHF88301
Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Human ST2 has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Human ST2 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for Human ST2 is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Human ST2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Specificity
Human ST2
Applications
Used for the quantitative determination of Human ST2 concentration in serum, plasma and cell culture supernatant.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
31.25 - 2,000 pg/mL
Sensitivity
21.22 pg/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
16
|
16
|
16
|
24
|
24
|
24
|
|
Mean (pg/mL)
|
949.7
|
242.2
|
60.8
|
978.8
|
237.3
|
57.3
|
|
Standard deviation
|
53.6
|
11.4
|
5.0
|
40.1
|
13.7
|
5.7
|
|
CV (%)
|
5.6
|
4.7
|
8.3
|
4.1
|
5.8
|
9.9
|
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Trans-resveratrol mitigates miR-204-3p mediated progression of allergic rhinitis by regulating the EGLN3/HIF-1α/IL33/ST2 signalling pathway., PMID:39226709
Postmortem biochemical analysis of soluble ST2 in the pericardial fluid of patients with sudden cardiac death caused by ischemic heart disease: a pilot study., PMID:37450171
Impact of Myocardial Ischemia and Revascularization by Percutaneous Coronary Intervention on Circulating Level of Soluble ST2., PMID:37434792
Factors associated with cardiac allograft vasculopathy after heart transplantation., PMID:36751283
Sex and age differences in sST2 in cardiovascular disease., PMID:36741845
Peri-implant crevicular fluid and serum levels of soluble ST2 in peri-implant diseases: A pilot study., PMID:36504319
Interleukin-33 and Soluble ST2 as Potential Biomarkers of Cancer in Opium Users: A Nested Case-Control Study., PMID:36380972
Regulatory Role of Zinc in Allergic Rhinitis through the IL-33/ST2 Pathway., PMID:35035826
ST2 and galectin-3 as novel biomarkers for the prediction of future cardiovascular disease risk in preeclampsia., PMID:34930081
[Preparation and identification of mouse-derived monoclonal antibodies to human ST2 molecule]., PMID:34809742
Elevated Serum Levels of Soluble ST2 Are Associated With Plaque Vulnerability in Patients With Non-ST-Elevation Acute Coronary Syndrome., PMID:34368249
Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases., PMID:34205541
IL-33/ST2 axis deficiency exacerbates neutrophil-dominant allergic airway inflammation., PMID:34178329
Synergistic relationship between TSLP and IL-33/ST2 signaling pathways in allergic rhinitis and the effects of hypoxia., PMID:31922361
Role of Zinc Sulphate in Immune Regulation in Artemisia annua Pollen-challenged P815 Mastocytoma Cells., PMID:31913054
Type 2 innate lymphoid cells participate in IL-33-stimulated Th2-associated immune response in chronic obstructive pulmonary disease., PMID:31572551
Role of the IL-33/ST2/p38 signaling pathway in the immune response of corneal epithelial cells to Aspergillus fumigatus infection., PMID:31024805
Evaluation of serum sST2 and sCD40L values in patients with microvascular angina., PMID:30502363
sST2 as a New Biomarker of Chronic Kidney Disease-Induced Cardiac Remodeling: Impact on Risk Prediction., PMID:30402040
Increased serum concentrations of soluble ST2 are associated with pulmonary complications and mortality in polytraumatized patients., PMID:29341938
IL-33 promotes food anaphylaxis in epicutaneously sensitized mice by targeting mast cells., PMID:27372570
The IL33/ST2 axis in Sjogren syndrome in relation to disease activity., PMID:27097949
Plasma soluble ST2 levels correlate with disease severity and predict clinical worsening in patients with pulmonary arterial hypertension., PMID:25068163
Soluble ST2 protein in the short-term prognosis after hospitalisation in chronic systolic heart failure., PMID:24846354
Predicting value of serum soluble ST2 and interleukin-33 for risk stratification and prognosis in patients with acute myocardial infarction., PMID:24112154
Soluble ST2 protein in chronic heart failure is independent of traditional factors., PMID:23515651
For research use only. Not for human or drug use.
