Catalog No.
KVV00305
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for SARS-CoV-2 RBD (KP.2) has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any SARS-CoV-2 RBD (KP.2) present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for SARS-CoV-2 RBD (KP.2) is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SARS-CoV-2 RBD (KP.2) bound in the initial step. The color development is stopped and the intensity of the color is measured.
Specificity
SARS-CoV-2 RBD (KP.2)
Applications
Used for the quantitative determination of SARS-CoV-2 RBD (KP.2) concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
23.44 - 1,500 pg/mL
Sensitivity
20.11 pg/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (pg/mL) |
696.9 |
163.3 |
38.7 |
697.0 |
170.1 |
37.0 |
|
Standard deviation |
25.1 |
3.7 |
2.3 |
26.6 |
9.1 |
4.5 |
|
CV (%) |
3.6 |
2.3 |
5.9 |
3.8 |
5.3 |
12.2 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
For research use only. Not for human or drug use.
